Preparation method of lysozyme

A technology of lysozyme and solution, applied in the direction of biochemical equipment and methods, enzymes, enzymes, etc., can solve the problems of long production cycle, environmental pollution, low resource utilization rate, etc.

Active Publication Date: 2009-12-16
SHANDONG LUBEI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional lysozyme production method, there are problems such as long production cycle, low product yield, low biological activity, low resource ut

Method used

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  • Preparation method of lysozyme

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Example Embodiment

[0022] Example 1

[0023] A method for preparing lysozyme, the steps of the method are:

[0024] a. Pretreatment: Clean the surface of fresh eggs with flowing water to remove debris and dirt, then soak them in a chlorine dioxide solution with a chlorine dioxide content of 20mg / L for 25 minutes. After soaking, use hot air to blow off the surface of the eggs. For water, use an egg beater to break the shell and separate the egg white and egg yolk. The egg white will enter the raw material storage tank after sterilization;

[0025] b. Separation and purification: Under stirring, dilute the egg white in the raw material storage tank with 0.8 times the weight of the egg white to obtain the egg white liquid, adjust the pH value of the egg white liquid to 6.5 with a dilute hydrochloric acid solution, and then use a plate and frame filter press Remove the precipitate and solid impurities in the egg white liquid, and then send it to a homogenizer for 15 minutes for homogenization. The homog...

Example Embodiment

[0028] Example 2

[0029] A method for preparing lysozyme, the steps of the method are:

[0030] a. Pretreatment: Use running water to spray to remove the sundries and dirt on the surface of the fresh eggs, and then soak them in a chlorine dioxide solution with a chlorine dioxide content of 30mg / L for 15 minutes. After soaking, use hot air to blow off the surface of the eggs. For water, use an egg beater to break the shell and separate the egg white and egg yolk. The egg white will enter the raw material storage tank after sterilization;

[0031] b. Separation and purification: Under stirring, dilute the egg white in the raw material storage tank with water 1.2 times the weight of the egg white to obtain the egg white liquid, adjust the pH value of the egg white liquid to 5.0 with a dilute hydrochloric acid solution, and then use a plate and frame filter press Remove the precipitates and solid impurities in the egg white liquid, and then send it to a homogenizer for 25 minutes for...

Example Embodiment

[0034] Example 3

[0035] A method for preparing lysozyme, the steps of the method are:

[0036] a. Pretreatment: Clean the surface of fresh eggs with flowing water to remove debris and dirt, and then soak them in a chlorine dioxide solution with a chlorine dioxide content of 25mg / L for 20 minutes. After soaking, use hot air to blow off the surface of the eggs. For water, use an egg beater to break the shell and separate the egg white and egg yolk. The egg white will enter the raw material storage tank after sterilization;

[0037]b. Separation and purification: Under stirring, dilute the egg white in the raw material storage tank with water 1.0 times the weight of the egg white to obtain the egg white liquid, adjust the pH value of the egg white liquid to 6 with a dilute hydrochloric acid solution, and then use a plate and frame filter press Remove the precipitate and solid impurities in the egg white liquid, and then send it to a homogenizer for 20 minutes for homogenization. Th...

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Abstract

The invention provides a preparation method of lysozyme, comprising the following steps: a. pretreating: separating egg white from egg yolk and facilitating the egg white to enter a raw material storage tank after sterilizing; b. separating and purifying: carrying out ultrafiltration separation on homogenized egg white liquid by an ultrafiltration membrane with molecular weight cutoff of 30000D to obtain filtered solution; sending the filtered solution to an ion exchange resin chromatography column for chromatography, carrying out elution after chromatography, collecting qualified elution solution and filtering the elution solution by an ultrafiltration membrane with molecular weight cutoff of 5000D to obtain concentrated solution cutoff; c. drying: spray drying the concentrated solution cutoff and preparing the finished product of lysozyme after drying. The product prepared by the method has higher enzyme activity than the enzyme activities of current domestic products, short production cycle and high yield, can be used as enzyme reagents of pharmaceutical raw materials and biochemical tools and can be also used as preservatives of high-grade organisms and food additives. Each index of the product reaches or exceeds the state pharmaceutical standard.

Description

technical field [0001] The invention provides a method for preparing lysozyme from eggs. Background technique [0002] Lysozyme (lysozyme), also known as muramidase (muramidase) or N-acetylmuramide lycanohydrlase (N-acetylmuramide lycanohydrlase), is a widely present in milk, saliva, tears, egg white and fish eggs Natural enzymes with high-efficiency antibacterial, bactericidal, and antiviral biological activities. It can decompose and kill Gram-positive bacteria such as Staphylococcus and Streptococcus. It can be used as a bactericidal and nutritional additive, widely used in the fields of medicine, food and biochemistry, and has a broad market prospect. Due to its high efficiency and wide range of pharmacological effects such as anti-virus, sterilization and anti-inflammation, the enzyme has become a research hotspot in the field of biomedicine. The content of lysozyme in egg white is the most abundant, and the resources are sufficient, so it becomes the first choice ra...

Claims

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Application Information

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IPC IPC(8): C12N9/36
Inventor 王克柳王美海杨红霞王立军颜丙辉
Owner SHANDONG LUBEI PHARMA
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