Method for fast detecting reverse transcription-polymerase chain reaction of paralichthys rhabdovirus

Abstract

The invention relates to the technical field of biology, disclosing a method for fast detecting the reverse transcription-polymerase chain reaction of paralichthys rhabdovirus. The method comprises the following steps of: designing a special primer; extracting RNA of a sample to be detected; having the reverse transcription-polymerase chain reaction; and judging a result by means of agarose gel electrophoresis. The method has the advantages of high speed, good accuracy and specificity and high sensitivity, is suitable for fast testing and quarantining the paralichthys rhabdovirus, and is applicable to aquafarms and disease inspection bureaus, thereby having a wide application prospect.

Description

Technical field:

[0001] The invention belongs to the detection and diagnosis technology of aquatic animal pathogens, in particular to the detection method of flounder rhabdovirus. Background technique:

[0002] The flounder rhabdovirus (Hirame rhabdovirus, referred to as HRV) virus particle is bullet-shaped, with a size of 80nm×160-180nm, which was first reported in Japan in 1986. This disease has been found in flounder bred in the Yellow Sea, Bohai Sea and other places in my country. The onset season is winter and early spring. When the fish body is onset, the water temperature is generally below 15°C. The disease mainly harms flounder, and artificial infection is highly pathogenic to red sea bream and black sea bream. The virus has also been isolated from sweetfish and unprepared flat carp, and it is also pathogenic to rainbow trout. Fish in the juvenile and larval stages are susceptible to this disease. Diseased fish infected with flounder rhabdovirus have hyperemia or...

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