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Muscodor sp. endophytic fungi ZJLQ024 and application thereof and fungicide

A technology of plant endophytic fungi and fungicides, applied in the direction of plant growth regulators, fungicides, botanical equipment and methods, etc., can solve problems such as irrational drug use, reduction in the number of natural enemies of harmful organisms, and poor environmental degradation ability

Active Publication Date: 2010-04-07
ZHEJIANG DAYANG BIOTECH GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemically synthesized agents has brought a series of serious consequences: 1) High residues: due to the carcinogenicity and toxicity of chemically synthesized agents, including various raw materials, it is not easy to get rid of them when entering animals and plants, and the environmental degradation ability is poor, resulting in pesticide residues High; 2) Destruction of ecological balance: long-term evolution and adaptation of organisms, the relative number of various organisms in nature has reached a dynamic balance, and the use of chemical agents has greatly reduced the number of natural enemies of harmful organisms; 3) The emergence of drug resistance: pathogenic bacteria and insects in nature The gene of the class has a strong ability to mutate, and irrational drug use and blind drug use promote the rapid emergence of drug resistance

Method used

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  • Muscodor sp. endophytic fungi ZJLQ024 and application thereof and fungicide
  • Muscodor sp. endophytic fungi ZJLQ024 and application thereof and fungicide
  • Muscodor sp. endophytic fungi ZJLQ024 and application thereof and fungicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Isolation and purification of endophytic fungal strains

[0042] (1) Collection of plant tissue specimens: from Fengyangshan National Nature Reserve, Zhejiang Province, China (Fengyangshan Nature Reserve is located in Longquan City, southwest Zhejiang Province, with a geographical location of 119°06′~119°15′E, 27 °46′~27°58′N, the climate is a typical mid-subtropical marine monsoon climate), and plant specimens, including Chinese goosebeery and Camptothecaacuminata, were collected at an altitude of 1200 to 1500m. Stems, leaves and fruits should be stored in fresh-keeping bags after collection, and stored at 4°C for no more than 48 hours.

[0043] (2) Isolation and culture of endophytic fungi: the ash layer and attachments on the surface of the plant specimen tissue of step (1) are washed with tap water, the surface water is dried, and the surface is disinfected by 75% absolute ethanol and 0.5% sodium hypochlorite. The time of sterilizing roots, stems, leaves...

Embodiment 2

[0044] Example 2. Screening of endophytic fungi producing volatile mixture VOCs

[0045] Using the confrontation culture method with a gridded petri dish, pour the melted PDA medium into a gridded plastic petri dish (90mm), cut out the endophytic fungus cake with a stainless steel hole punch with a diameter of 5mm, and connect it to one side of the petri dish. After culturing at ℃ for 2d-5d, insert the pathogenic indicator bacteria on the other side of the PDA plate. At the same time, take only the pathogenic indicator bacteria as the control, and seal it with parafilm. The growth and bacteriostasis of the indicator pathogen colonies by the fungi were measured, and the colony diameter of the indicator bacteria was measured, and the endophytic fungi that could produce volatile mixture VOCs and inhibit the growth of plant pathogens were screened out.

[0046] The 158 isolated and purified endophytic fungal strains were screened for biological activity. Botrytis cinerea, Rhizocto...

Embodiment 3

[0047] Embodiment 3, the test of the antibacterial activity spectrum of Muscodor sp. strain ZJLQ024

[0048] The endophytic fungi Muscodor sp. strain ZJLQ024 and Muscodor albus (as disclosed in the patent 200480025237.X) were grown and activated on PDA medium for 10-15 d (cultivation temperature was 25° C.). Botrytis cinerea, Rhizoctonia solani, Alternaria sclerotiorum, Sclerotinia sclerotiorum, Pythium ultimum, Fusarium oxysporum cucumber specialization, Coccidioides spinosa, Verticillium dahliae, Sclerotinia sclerotiorum , Polychaete persimmons, Pyromyces oryzae, Penicillium dactylus, Botrytis faba, Nitrosporium, Aspergillus, Paecilomyces lilacinus were activated on PDA medium and transferred to PDA fresh On the medium, grow at 25°C for 3-7 days before use; Saccharomyces cerevisiae is streaked on the YEPD solid medium, single colonies are picked, and the bacteria are inoculated in the YEPD liquid culture medium by shaking (200r / min, 30°C), after 48h Use; Escherichia coli an...

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Abstract

The invention discloses endophytic fungi Muscodor sp. The preservation name of the strain is ZJLQ024, the preservation unit is China General Microbiological Culture Collection Center, the preservation date is January 8, 2009 and the preservation number is CGMCC 2863. The endophytic fungi can be prepared into living preparations and can be used for suppressing or killing pathogenic microorganisms or musty microorganisms. The invention simultaneously discloses a fungicide comprising 2-methyl-methyl propionate, 2-methylpropionate and the like, especially comprising alpha-phellandrene, beta-phellandrene, trans1-methyl-4-(1-methyl ethyl)-2-cyclohexene-1-alcohol, cis1- methyl-4-(1-methyl ethyl)-2-cyclohexene-1-alcohol, trans3-methyl-6-(1-methyl ethyl)-2-cyclohexene-1-alcohol and cis3-methyl-6-(1-methyl ethyl)-2-cyclohexene-1-alcohol.

Description

technical field [0001] The present invention relates to the field of microbiology and fungicides, in particular to a new endophyte of the genus Muscodor and volatile mixtures based on or derived therefrom and their uses. Background technique [0002] Fungi is a huge family of microorganisms. The relationship between fungi and humans is very close. Fungi can be used to manufacture important products in industry and agriculture such as medicine, food, and chemical industry. The famous penicillin and cephalosporin are so far one of the most precious drugs that fungi have brought to the medical world, and they are also the most important antibiotics at present. They are widely used in the treatment of bacterial infections and have saved millions of human lives. life. [0003] Endophytic fungi are an important group of fungi. According to Dreyfuss and Chapel, the total number of endophytic fungi is estimated to be 1 million. Few species are now used in medicine, agronomic produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P1/00A01P3/00A01N37/02A01N31/06A01N27/00C12R1/645
Inventor 林福呈王国平章初龙毛黎娟周转忠
Owner ZHEJIANG DAYANG BIOTECH GROUP
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