Microbial preparation for controlling and eliminating mastitis pathogenic bacterium and application thereof
A technology of microbial preparations and pathogenic bacteria, applied in the direction of microorganisms, methods based on microorganisms, chemicals for biological control, etc., can solve the problems of hindering the action of drugs, polluting milk, and consumption of disinfectants, etc., achieving remarkable application effects, Wide application range and strong bactericidal effect
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Embodiment 1
[0036] (1) Preparation of microbial preparations for controlling and eliminating mastitis pathogenic bacteria
[0037] The Bdellovibrio strains used were Bdellovibrio BDS01 and Bdellovibrio BDS02, respectively. Bdellovibrio BDS01 was preserved in the China Center for Type Culture Collection on August 7, 2009, and the preservation number is CCTCC NO: M209169; after negative staining of the Bdellovibrio BDS01, the morphology was observed under an electron microscope: BDS01 was a single cell , arc-shaped, with a size of 0.9×0.25 μm, terminal flagella, and the length of the flagella is 4 μm, which can form transparent circular plaques with a diameter of 1-2 mm when cultured at 28 ° C for four days by double-layer plate method. Bdellovibrio BDS02 was preserved in the China Center for Type Culture Collection on August 7, 2009, and the preservation number is CCTCCNO: M 209170; after negative staining of the Bdellovibrio BDS02, the morphology was observed under an electron microscope:...
Embodiment 2
[0061] (1) Preparation of microbial preparations for controlling and eliminating mastitis pathogenic bacteria
[0062] The preparation process is the same as step (1) of Example 1, the only difference being that adding physiological saline to resuspend the Bdellovibrio sediment, the concentrations of the Bdellovibrio BDS01 swimming body bacterial liquid and the Bdellovibrio BDS02 swimming body bacterial liquid are respectively 10 2 pfu / ml, mix the Bdellovibrio BDS01 swimming body bacterial liquid and the Bdellovibrio BDS02 swimming body bacterial liquid according to the number of bacteria in 1:1 to obtain the microbial preparation.
[0063] (2) Application of microbial preparations to eliminate mastitis pathogenic bacteria in dairy farming environment
[0064] A. Prepare conventional disinfectant as a control group
[0065] Prepare 4%wt cresol soap with sterile saline.
[0066] B. The effect of conventional disinfectants and microbial agents on the dairy farming environment ...
Embodiment 3
[0078] (1) Preparation of microbial preparations for controlling and eliminating mastitis pathogenic bacteria
[0079] The preparation process is the same as in Example 1 step (1), the difference is only that adding 0.2mol / L, the potassium hydrogen phosphate buffer (PBS) with a pH value of 7.2 resuspends the Bdellovibrio sediment, the Bdellovibrio BDS01 swimming body bacterial liquid and The concentrations of the Bdellovibrio BDS02 swimming body bacterial liquid were 10 3 pfu / ml, mix the Bdellovibrio BDS01 swimming body bacterial liquid and the Bdellovibrio BDS02 swimming body bacterial liquid according to the number of bacteria in 1:1 to obtain the microbial preparation.
[0080] (2) Applying microbial preparations for the prevention and treatment of mastitis in dairy cows to eliminate pathogenic bacteria on the mammary glands of dairy cows
[0081] Gently scrub the mammary glands of ten dairy cows with a small sterile brush and 200 mL of sterile saline to make a bacterial s...
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