Microbial preparation for controlling and eliminating mastitis pathogenic bacterium and application thereof

A technology of microbial preparations and pathogenic bacteria, applied in the direction of microorganisms, methods based on microorganisms, chemicals for biological control, etc., can solve the problems of hindering the action of drugs, polluting milk, and consumption of disinfectants, etc., achieving remarkable application effects, Wide application range and strong bactericidal effect

Inactive Publication Date: 2010-04-14
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these conventional disinfectants are in direct contact with organic matter such as cow dung and feed residues, causing the disinfectant to be consumed by a large amount of organic matter, hindering the drug's effect, greatly reducing the killing effect of the drug on pathogenic...

Method used

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  • Microbial preparation for controlling and eliminating mastitis pathogenic bacterium and application thereof
  • Microbial preparation for controlling and eliminating mastitis pathogenic bacterium and application thereof
  • Microbial preparation for controlling and eliminating mastitis pathogenic bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Preparation of microbial preparations for controlling and eliminating mastitis pathogenic bacteria

[0037] The Bdellovibrio strains used were Bdellovibrio BDS01 and Bdellovibrio BDS02, respectively. Bdellovibrio BDS01 was preserved in the China Center for Type Culture Collection on August 7, 2009, and the preservation number is CCTCC NO: M209169; after negative staining of the Bdellovibrio BDS01, the morphology was observed under an electron microscope: BDS01 was a single cell , arc-shaped, with a size of 0.9×0.25 μm, terminal flagella, and the length of the flagella is 4 μm, which can form transparent circular plaques with a diameter of 1-2 mm when cultured at 28 ° C for four days by double-layer plate method. Bdellovibrio BDS02 was preserved in the China Center for Type Culture Collection on August 7, 2009, and the preservation number is CCTCCNO: M 209170; after negative staining of the Bdellovibrio BDS02, the morphology was observed under an electron microscope:...

Embodiment 2

[0061] (1) Preparation of microbial preparations for controlling and eliminating mastitis pathogenic bacteria

[0062] The preparation process is the same as step (1) of Example 1, the only difference being that adding physiological saline to resuspend the Bdellovibrio sediment, the concentrations of the Bdellovibrio BDS01 swimming body bacterial liquid and the Bdellovibrio BDS02 swimming body bacterial liquid are respectively 10 2 pfu / ml, mix the Bdellovibrio BDS01 swimming body bacterial liquid and the Bdellovibrio BDS02 swimming body bacterial liquid according to the number of bacteria in 1:1 to obtain the microbial preparation.

[0063] (2) Application of microbial preparations to eliminate mastitis pathogenic bacteria in dairy farming environment

[0064] A. Prepare conventional disinfectant as a control group

[0065] Prepare 4%wt cresol soap with sterile saline.

[0066] B. The effect of conventional disinfectants and microbial agents on the dairy farming environment ...

Embodiment 3

[0078] (1) Preparation of microbial preparations for controlling and eliminating mastitis pathogenic bacteria

[0079] The preparation process is the same as in Example 1 step (1), the difference is only that adding 0.2mol / L, the potassium hydrogen phosphate buffer (PBS) with a pH value of 7.2 resuspends the Bdellovibrio sediment, the Bdellovibrio BDS01 swimming body bacterial liquid and The concentrations of the Bdellovibrio BDS02 swimming body bacterial liquid were 10 3 pfu / ml, mix the Bdellovibrio BDS01 swimming body bacterial liquid and the Bdellovibrio BDS02 swimming body bacterial liquid according to the number of bacteria in 1:1 to obtain the microbial preparation.

[0080] (2) Applying microbial preparations for the prevention and treatment of mastitis in dairy cows to eliminate pathogenic bacteria on the mammary glands of dairy cows

[0081] Gently scrub the mammary glands of ten dairy cows with a small sterile brush and 200 mL of sterile saline to make a bacterial s...

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Abstract

The invention discloses a microbial preparation for controlling and eliminating mastitis pathogenic bacterium and application thereof. The microbial preparation for controlling and eliminating the mastitis pathogenic bacterium is prepared from Bdellovibrio bacteriovorus telotroch. Through detection, the microbial preparation can effectively eliminate common mastitis pathogenic bacterium of milk cows, including colibacillus, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae and the like existing in the environment. Compared with Bdellovibrio bacteriovorus leech plastid, the microbial preparation has the weaknesses of fussy preparation and difficult storage, but has the advantages of rapid effect and strong activity by using the Bdellovibrio bacteriovorus telotroch to control and eliminate the mastitis pathogenic bacterium. The Bdellovibrio bacteriovorus telotroch has no side or toxic effect for the milk cows so that the microbial preparation prepared from the Bdellovibrio bacteriovorus telotroch is suitable for large-scale industrial culture of milk cows, provides guarantee for green processing and production of the milk cows and provides a new approach for controlling and eliminating the mastitis pathogenic bacterium of the milk cows.

Description

technical field [0001] The invention relates to a microbial preparation, in particular to a microbial preparation for controlling and eliminating mastitis pathogenic bacteria and its application. Background technique [0002] Dairy cow mastitis (also called mastitis) is one of the diseases with the highest incidence and the most serious losses in dairy farming. According to statistics, clinical mastitis accounts for 21% to 23% of the total incidence of dairy cows, and recessive mastitis is more common in dairy herds, with an incidence rate as high as 50%. The occurrence of mastitis in dairy cows has a serious impact on the yield and quality of milk, and has caused extremely serious economic losses to the development of cattle breeding. [0003] Pathogen infection is the main cause of mastitis, including bacteria, fungi, viruses, mycoplasma, etc. The main pathogenic bacteria are bacteria, the common ones are Staphylococcus aureus, Escherichia coli, Streptococcus and mycopla...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P15/14A01N63/02A01P1/00A23K1/18A23L1/015C02F3/34C12R1/01A23K10/18A23K50/10A23L5/20
Inventor 蔡俊鹏鲁锋
Owner SOUTH CHINA UNIV OF TECH
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