Membrane matrix protein chip for detecting mycotoxin and preparation method thereof

Abstract

The invention relates to a membrane matrix protein chip for detecting mycotoxin and a preparation method thereof. The membrane matrix protein chip for detecting mycotoxin comprises a polyvinylidene fluoride membrane substrate and six spotting substances of bovine serum albumin conjugates of aflatoxin B1, zearalenone, ochratoxin A, deoxynivalenol, fumonisins B1 and fumonisins B2. The preparation method comprises the following steps: after soaking the polyvinylidene fluoride membrane in methanol, leveling the membrane on the upper end of filter paper; spotting the spotting substances on the membrane by a spotting instrument; after incubation, closing redundant sites which do not combine with the spotting substances on the membrane by hybridprotein; and carrying out vacuum encapsulation after washing and airing. The invention has the advantages of simple operation, convenience, high speed, determination of various types of mycotoxin at a time without any instrument and equipment, long-term storage, simple preparation process and large-scale production and has the characteristics of high sensitivity of ELISA, discrimination by naked eye of the colloidal gold immunity chromatography technology and high flux detection of biochips.

Description

technical field [0001] This patent relates to the field of biotechnology, and specifically relates to a membrane-based protein chip that can detect multiple common and important mycotoxins at the same time, and can be used for rapid detection of mycotoxins in grains, feed and food. Background technique [0002] Mycotoxins are secondary metabolites produced by fungi, and more than 200 different mycotoxins are currently known. Due to the parasitism of fungi and the production of mycotoxins, the yield of crops is seriously affected, the quality of agricultural products and feed is reduced, and huge economic losses are caused. Worldwide losses of agricultural products and industrial raw materials due to mycotoxins amount to tens of billions of dollars each year. According to the survey, in addition to food and feed, mycotoxins have been found in varying degrees in oil crop seeds, fruits, dried fruits, vegetables, condiments, tobacco, hemp, milk and dairy products, fish and shri...

AI Technical Summary

Problems solved by technology

High-performance liquid chromatography and gas chromatography-mass spectrometry have the characteristics of accurate detection and good repeatability. The use is greatly restricted and is not suitable for widespread use at the grassroots level

Enzyme-linked immunosorbent assay is a commonly used large-scale screening method at present, but the enzyme-linked immunosorbent assay also requires the cooperation of instruments, and the detection time is too long, which is difficult to meet the needs of rapid on-site detection at the grassroots level. Colloidal gold immunochromatography is used as Common immunological diagnostic methods have been widely used in agriculture and biomedical fields, especially in medical testing. It is easy to operate, fast, and does not require instrumental testing to interpret the results. It is suitable for grassroots testing

[0004] None of the above methods can detect multiple mycotoxins in the sample at the same time. However, mycotoxins are often mixed contamination, and it is more common for samples to be contaminated with multiple mycotoxins at the same time.