Pseudomonas Flava for increasing yield of medicinal plant, namely bulbus fritillariae pallidiflorae and application thereof

A technology of Pseudomonas and medicinal plants, which is applied in microbial preparations, bacteria that increase the yield of medicinal plants Ignimus chinensis and its application fields, and can solve the problems of unscaled scale, no reports of bacterial agents, and large demand , to achieve the effect of increasing production

Inactive Publication Date: 2011-11-09
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, due to the positive curative effect of Fritillaria, it has been paid more and more attention by the medical industry. The demand is large, resulting in a shortage of supply. Due to market economic factors, the wild resources have been severely reduced, and the variety has been mixed, which has affected the medicinal value.
Although there is a certain area of ​​artificial planting at present, it has not yet reached a large scale, and there are also current conditions such as low output.
[0003] After searching, domestic and foreign patents in the field of Fritillaria Ili mainly focus on the tissue culture and breeding of Fritillaria Ilii, for example, the patent application number is 200710181008.2, and the polyploid Fritillaria Ili is obtained through screening and identification of polyploids. To overcome the problem of species degeneration in the long-term planting of Fritillaria Ili (Wang Xiaojun, a method for inducing polyploidy of Fritillaria Ili under in vitro culture, Publication No. CN 101161056A), there is no report on the bacterial agent for increasing the yield of Fritillaria Ili

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment one: the isolation and cultivation of bacterial strain

[0015] Preparation of the soil extract: put 100g of the iberia rhizosphere soil sample into 1000mL of tap water, boil it, and filter it for later use. Strain isolation and purification medium in g / L, including beef extract 3-5, peptone 8-10, yeast extract 4-5, sodium chloride 4-5, glucose 4-5, agar 11-13, pH7 .0-7.2, the culture medium is prepared with the above soil extract, and sterilized at 120°C for 30min. Strain isolation and purification adopts the plate dilution separation method, and cultures at 25-35°C for 36-48 hours.

[0016] Isolate a strain of rod-shaped bacteria from the rhizosphere soil of Igami japonicus by the above method, about 1.5 μm in length, Gram-negative, egg yellow, 1-5 mm in diameter, use arabinose, sucrose and maltose, and do not use gluconic acid Salt, paraben and pantothenate, aerobic, catalase positive, G+C = 67.3% in DNA.

Embodiment 2

[0017] Embodiment two: the taxonomic identification of bacterial strain

[0018] By analyzing the individual morphology, colony characteristics and physiological and biochemical characteristics of the strains obtained in Example 1, according to the eighth edition of "Bergey's Manual of Systematic Bacterio-logy" ("Bergey's Manual of Systematic Bacterio-logy") and "Common Bacteria System Identification Manual", through microbiological classification and identification, the strain provided by the present invention is Pseudomonas Flava.

Embodiment 3

[0019] Example Three: Application of Pseudomonas Flava (Pseudomonas Flava) CGMCC.No.3506 in Improving the Medicinal Plant Iberia

[0020] The Pseudomonas Flava (Pseudomonas Flava) CGMCC.No.3506 identified in Example 1 Separation and Purification Example 2 was inoculated in 1000 mL by 5 mL of bacterium suspension (take a ring of slant bacteria and put 10 mL of sterile water to obtain the bacterium suspension) In the liquid culture medium, shake culture at 25-35°C for 36-48 hours to become a liquid microbial agent.

[0021] The liquid medium for making bacterial agents is calculated in g / L, including corn flour 18-20, bean cake flour 19-21, bran 28-30, dipotassium hydrogen phosphate 0.02-0.04, disodium hydrogen phosphate 0.02-0.04, sulfuric acid Magnesium 0.01-0.03, calcium chloride 0.02-0.05, prepared with tap water, pH 7-7.2, sterilized at 120°C for 30 minutes in the liquid culture component of the bacterial agent.

[0022] Shake culture to obtain a liquid culture, and in the...

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Abstract

The invention discloses Pseudomonas Flava CGMCC No.3506 and a microbial inoculum for increasing yield of a medicinal plant, namely bulbus fritillariae pallidiflorae. The invention provides a preparation obtained by adopting the Pseudomonas Flava CGMCC No.3506 through a conventional fermentation method. Based on g / L, a culture medium comprises the following components: 18 to 20g of corn flour, 19 to 21g of soybean cake powder, 28 to 30g of bran, 0.02 to 0.04g of dipotassium hydrogen phosphate, 0.02 to 0.04g of disodium hydrogen phosphate, 0.01 to 0.03g of magnesium sulfate, 0.02 to 0.05g of calcium chloride, and the balance of tap water, wherein the pH value is between 7.0 and 7.2. After root irrigation in a period of seedling establishment of the planted bulbus fritillariae pallidiflorae,compared with a control test group, the yield of the bulbus fritillariae pallidiflorae can be increased by 23.1 percent; and the microbial inoculum has important application prospect on increasing the yield of the medicinal plant, namely the bulbus fritillariae pallidiflorae.

Description

field of invention [0001] The present invention relates to the technical field of microbial preparations, in particular, the present invention relates to a bacterium for increasing the yield of medicinal plant Ignifera and the technical field of application thereof. Background technique [0002] Authentic medicinal materials are a major feature of traditional Chinese medicine, especially those famous and authentic medicinal materials from specific production areas. Fritillaria is a perennial herb of the family Liliaceae, mainly produced in Sichuan, Xinjiang, Northeast, North China, Northwest, and Southwest And East China and other places, Bulbus Fritillariae Pallidiflorae is the dried bulb of Fritillaria walu-iewii Regel or Fritillaria Pallid iflora Schrenk. The main components of Ignebra are alkaloids, which mainly include D, E-ring dihydrocis-sibecin and new steroidal alkaloids, such as neobekine, among which D, E-ring dihydro-cis-sibecin Belonging to cevine groups, it is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/38
Inventor 潘惠霞程争鸣包群牟书勇齐晓玲
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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