Method for preparing modified coatings of imitating cell outer-layer membrane structure

A technology imitating the outer membrane and coating of cells, which is applied in the fields of polymer chemistry and physics and biomedical materials, can solve problems such as the instability of amphiphilic polymer coatings, and achieve good blood compatibility and stability, Strong hydrophilicity and simple preparation process

Active Publication Date: 2010-06-16
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing a coating imitating the outer membrane structure of cells, to solve the problems of instability of the usual amphiphilic polymer coatings and the formation of reverse outer membrane structures, and to obtain more hydrophilic coatings. , Cross-linked and more stable imitation cell outer membrane structure surface

Method used

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  • Method for preparing modified coatings of imitating cell outer-layer membrane structure
  • Method for preparing modified coatings of imitating cell outer-layer membrane structure
  • Method for preparing modified coatings of imitating cell outer-layer membrane structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take the monomer 2-methacryloyloxyethylphosphorylcholine (MPC) and γ-methacryloyloxypropyltrimethoxysilicon (TSMA) according to a certain ratio, dissolve them in isopropanol, and weigh the monomers The initiator azobisisobutyronitrile (AIBN) with a total mass of 1% was dissolved with a small amount of THF, mixed with the monomer solution, and loaded into a dropping funnel. in N 2 Under protection and stirring at a constant temperature of 80°C, the mixture of monomer and initiator was added dropwise, and the drop was completed within 3 hours. After the dropwise addition was completed, the system was sealed for reaction. After the reaction was carried out for 10 hours, a THF solution of 0.02% of the total monomer mass was added, and the heating and stirring was stopped after the reaction was carried out for 24 hours. Reaction solution in N 2 Filtrated under protection, the filtrate was concentrated by rotary evaporation and precipitated with cold anhydrous ether, the pr...

Embodiment 2

[0021] The cross-linkable polymer synthesized in Example 1 with a ratio of MPC and TSMA of 80:20 was prepared into a 1 mg / mL acetonitrile solution. The solution was sprayed or dipped onto a dry and clean cover glass surface, and after vacuum drying, it was treated in a saturated atmosphere of 2% triethylamine aqueous solution at 90°C for 1 hour to obtain a water-insoluble cross-linked coating. With pure water as the wetting medium, the test temperature is 20°C, the test speed of the advancing angle and the receding angle are both 0.05mm / s, the surface tension measurement threshold is 5.00mg, the immersion depth is 10.00mm, and the test point frequency is 5.00Hz. The angle is 45±2°, and the receding angle is 5±1°.

[0022] In the comparison test, after vacuum drying, the coating was still dissolved in a saturated atmosphere of pure water at 90°C for 1 hour, indicating that the crosslinking was not complete. After treatment at 120°C for 1 hour, the coating no longer dissolves, ...

Embodiment 3

[0024] Take the cross-linkable polymer synthesized in Example 1 with a ratio of 90:10 between MPC and TSMA, and prepare a 2 mg / mL isopropanol solution. The solution was dip-coated on a dry and clean cover glass surface, and after vacuum drying, it was treated in a saturated atmosphere of 0.8% trimethylamine aqueous solution for 10 h at 50° C. to obtain a cross-linked coating imitating the membrane structure of the outer layer of cells. With pure water as the wetting medium, the test temperature is 20°C, the test speed of the advancing angle and the receding angle are both 0.05mm / s, the surface tension measurement threshold is 5.00mg, the immersion depth is 10.00mm, and the test point frequency is 5.00Hz. The angle is 41±2°, and the receding angle is 4±2°.

[0025] In the comparison test, after vacuum drying, the coating was still dissolved in a saturated atmosphere of pure water at 50°C for 10 hours, indicating that the crosslinking was not complete. After treatment at 100°C ...

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Abstract

The invention discloses a method for preparing imitating cell outer-layer membrane structure coatings. The method comprises the followings steps: (1) dissolving binary copolymers expressed by a structural general formula (I) in anhydrous polar organic solvent in order to obtain solution in a concentration of 1 to 10 mg/mL; (2) allowing polymers to form a uniform coating on the surface of a modified substrate by a spraying or dip-coating method; and (3) drying the coating under vacuum, treating the obtained product in ammonia water in a weight-percentage concentration of 0.5 to 5.0 percent or saturated steam of volatile organic amine aqueous solution for 1 to 72 hours at the temperature of between 20 and 120 DEG C to obtain a cross-linked fixed hydrophilic surface of the imitating cell outer-layer membrane structure. The more hydrophilic, cross-linked and stable surface of the imitating cell outer-layer membrane structure can be obtained after the obtained product is treated in the ammonia water or the saturated steam of volatile organic amine aqueous solution.

Description

technical field [0001] The invention relates to a method for preparing a coating imitating a cell outer layer membrane structure by using a copolymer imitating a cell membrane outer layer structure, and belongs to the technical fields of macromolecule chemistry and physics and biomedical materials. Background technique [0002] With the rapid development of modern medicine, various minimally invasive interventional medical devices such as vascular stents, sensors, and cardiac assist devices have been widely used in various medical technologies, greatly enriching the means of modern medical diagnosis and treatment. However, in clinical applications, existing devices still have infection, coagulation, postoperative tissue hyperplasia and other immune side effects to varying degrees, which bring great pain to patients, increase medical expenses, and sometimes require reoperation . Currently, there are three main methods used to improve the hemocompatibility of implant material...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/10
Inventor 杨珊王彦兵宫铭张静梁晓进宫永宽
Owner NORTHWEST UNIV(CN)
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