Albumin/keratin double expression mice embryonic stem cell system and establishing method thereof

A mouse embryo and keratin technology, applied in the field of genetic engineering, can solve the problems of identifying the surface marker proteins of hepatic progenitor cells, difficulty in tracking and sorting hepatic progenitor cells, and inability to accurately demarcate hepatic progenitor cells, etc. Practical, simple effect
CN101760450BActive Publication Date: 2013-04-10CENT FOR EXCELLENCE IN MOLECULAR CELL SCI CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CENT FOR EXCELLENCE IN MOLECULAR CELL SCI CHINESE ACAD OF SCI
Publication Date
2013-04-10

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Abstract

The invention discloses an albumin / keratin double expression mice embryonic stem cell system and an establishing method thereof. The mice embryonic stem cell system of the invention is established by sub-culturing the albumin / keratin double expression mice embryonic stem cell. The mice embryonic stem cell is integrated with a red fluorescence / green fluorescence protein double reporter gene, wherein the reporter gene is regulated by tissue specificity promoter albumin ALB or keratin CK19. Compared with a method of inputting genes, the method of controlling the reporter gene by using the transfection tissue specificity promoter of the invention is more simple, rapid and useful. The uniform originator cells in the developmental stage can be precisely chosen by using the albumin / keratin double expression mice embryonic stem cell of the invention, which can bring a great beneficial effect to the following research on the originator cell differentiation, the cell transplanting and cure.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering, and in particular relates to an albumin / keratin (ALB / CK19) double-expression mouse embryonic stem cell line and a method for establishing the same. Background technique

[0002] Embryonic stem cells (ESCs) are totipotent stem cells isolated from the inner cell mass of mammalian blastocysts, which can proliferate indefinitely and differentiate into more than 200 cell types throughout the body. Embryonic stem cells can form embryoid bodies (EBs) in suspension culture. Embryooid bodies are spheres similar to early embryos formed spontaneously by embryonic stem cells under certain conditions in vitro. The formation and differentiation of the three germ layers basically simulate the early development of embryos in vivo. The differentiation process of tissue cells. In 1996, Japanese scholar Abe et al. first detected the expression of endoderm genes during embryoid body development, and its expressio...

Claims

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