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Magnetic immunochromatographic strip for detection of hepatitis B surface antigen and preparation method thereof

A technology of hepatitis B surface antigen and magnetic immunochromatography, which is applied in the field of medical testing, can solve problems such as long reaction time, long test cycle, and improved sensitivity, and achieve the effects of simplifying the preparation process, reducing subjectivity, and accurate results

Inactive Publication Date: 2010-06-30
CHEMCLIN DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] ELISA is currently the most commonly used detection technology in clinical blood screening, but ELISA test procedures are complex, prone to false positive or false negative results, and low sensitivity, CLIA is similar to ELISA, but the sensitivity is improved, and there is no fundamental solution There are problems in ELISA, and both of them have the problems of cumbersome operation and long reaction time, and both need complex equipment such as microplate reader or luminometer, plate washer and incubator, and cannot be tested by a single person, which further limits their Application in some primary hospitals and clinics
In recent years, rapid detection test strips represented by colloidal gold or latex particles have appeared at home and abroad, but because the results are visually observed by the naked eye, they are easily affected by the subjective judgment of the observer, the sensitivity is low, and the accuracy of the results is not high. Long detection window
[0009] The PCR method requires a very high experimental working environment and conditions (sterile operation room, ultra-clean workbench) and a large number of instruments and equipment (incubator, centrifuge, PCR instrument, etc.), and the operation is extremely complicated, requiring strict professional training Personnel operation, and the test period is very long, so it is not suitable for clinical testing

Method used

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  • Magnetic immunochromatographic strip for detection of hepatitis B surface antigen and preparation method thereof
  • Magnetic immunochromatographic strip for detection of hepatitis B surface antigen and preparation method thereof

Examples

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Effect test

Embodiment 1

[0037] Preparation method of magnetic immunochromatography test strip and test paper box for detecting hepatitis B surface antigen in blood

[0038] The preparation method of test strip and test paper box of the present embodiment comprises the following steps:

[0039] A. Antibody preparation: select commercial HBsAb, dialyze against 20 mM PBS with pH 7.2 (pH 7.0-7.6 is applicable), and dialyze overnight at 4° C. for later use.

[0040] B. Preparation of coating film:

[0041] Coating buffer preparation: 0.02M phosphate buffer solution (PBS) with pH 7.2 was used as the coating buffer, which was sterilized by 0.22 μm microporous membrane and stored at 4°C for later use, with a validity period of two weeks.

[0042] Preparation of blocking solution: 0.02M phosphate buffered saline (PBS) containing 0.5% BSA at pH7.2 (pH7.0-7.6 is applicable), 0.22μm microporous membrane filter and sterilize, and store at 4°C for later use. Valid for one week.

[0043] Preparation of coating f...

Embodiment 2

[0070] Except for the mixing step of FITC-HBsAb and anti-FITC antibody magnetic particles: after thorough mixing, the mixture was diluted with storage buffer at a ratio of 1:10 to be sprayed on glass fiber mats, other steps were the same as in Example 1.

Embodiment 3

[0072] Except for the mixing step of FITC-HBsAb and anti-FITC antibody magnetic particles: after thorough mixing, the mixture was diluted with storage buffer at a ratio of 1:20 to be sprayed on glass fiber mats, other steps were the same as in Example 1.

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Abstract

The invention relates to a magnetic immunochromatographic strip for detection of hepatitis B surface antigens and a preparation method thereof. The strip is prepared through the following steps: pasting a coating film, a magnetic particle pad combined with hepatitis B surface antibodies, a sample pad and an absorbent pad on a bottom board in sequence at intervals of 2mm, and using a transparent plastic seal film to cover the upper layer. The coating film is pre-coated with hepatitis B surface antigen detection lines and quality control lines. The invention introduces the magnetic immunochromatographic technique and the fluorescein isothiocyanate system into the detection of the hepatitis B surface antigens, and has the advantages of simple operation, high sensitivity and good specificity.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to a magnetic immunochromatographic test strip for detecting hepatitis B surface antigen and a preparation method thereof. Background technique [0002] Hepatitis is a general term meaning inflammation of the liver and it can be caused by a range of different viruses such as hepatitis A, B, C, D and E viruses. Among the many viruses that cause hepatitis in humans, hepatitis B virus (HBV) has the most global impact. [0003] Hepatitis B is caused by hepatitis B virus (HBV), an enveloped virus with a partially double-stranded circular DNA genome, belonging to the Hepadnaviridae family. When the virus replicates in liver cells, it can interfere with liver function, and the immune system activates a series of specific responses to fight and eradicate the infectious agent. As a result of pathological damage, the liver becomes inflamed. Severe pathological consequences of persistent HBV ...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/533
Inventor 唐宝军应希堂李强胡国茂郑金来于尚永
Owner CHEMCLIN DIAGNOSTICS CO LTD
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