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Method for cultivating sweet sorghum tissue and special culture medium thereof

A technology of sweet sorghum and culture medium, applied in the field of sweet sorghum tissue culture, method and its special medium, which can solve the problems of low regeneration efficiency and difficulty in hardening seedlings, and achieve high regeneration efficiency, normal shape and good genetic stability Effect

Active Publication Date: 2012-07-18
FUJIAN SANAN SINO SCI PHOTOBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are reports at home and abroad on the establishment of sorghum regeneration systems using sorghum young spikes, immature embryos, mature seeds, shoot apex meristems, mature embryos, etc. as explants, due to genotype-dependent reactions, phenolic or pigment substances produced, its regeneration efficiency is low and it encounters problems such as difficulties in hardening seedlings

Method used

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  • Method for cultivating sweet sorghum tissue and special culture medium thereof
  • Method for cultivating sweet sorghum tissue and special culture medium thereof
  • Method for cultivating sweet sorghum tissue and special culture medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, acquisition of regenerated seedlings of sweet sorghum and statistical observation thereof

[0052] 1. Obtaining regenerated seedlings of sweet sorghum

[0053] 1. Preparation and statistical observation of callus

[0054] 1) Pretreatment of explants

[0055] The young panicles (Phase IV-VI of young panicle differentiation) of sweet sorghum variety Keller (Sorghum bicolor (L.) Moench 'Keller') were used as explants.

[0056] The specific operation of pretreatment: first select the robust sweet sorghum plants, take the sweet sorghum young ears with leaves less than 10cm in length, add detergent to clean the dust on the surface of the material, rinse it under running tap water for about 30 minutes, and then put it on Carry out disinfection in the ultra-clean workbench, first sterilize with 75% (volume percentage) ethanol for 2-3 minutes, then sterilize with 0.1% (mass percentage) mercury chloride for 10-15 minutes, and finally wash with sterile water for 4 -...

Embodiment 2

[0098] Embodiment 2, the acquisition of sweet sorghum regenerated seedlings

[0099] The difference between this embodiment and embodiment 1 is the callus induction medium, and all the other steps are identical:

[0100] The callus induction medium of the present embodiment is obtained by adding hydrolyzed casein, proline, polyvinylpyrrolidone (PVP), vitamin C, 2,4-D, sucrose and agar on the basis of MS basic culture medium Medium, wherein the final concentration of hydrolyzed casein is 0.5g / L, the final concentration of proline is 0.6g / L, the final concentration of PVP is 100mg / L, and the final concentration of vitamin C is 10mg / L, 2, The final concentration of 4-D is 2.0mg / L, the final concentration of ZT is 1.0mg / L, the final concentration of sucrose is 30g / L, and the final concentration of agar is 8g / L; The pH of this medium is 5.8; Wherein MS The solvent of the basic culture medium is water, and the solute is shown in Table 1.

[0101] The experimental results are shown...

Embodiment 3

[0104] Embodiment 3, the acquisition of sweet sorghum regenerated seedlings

[0105] The difference between this example and Example 1 is that the callus induction medium used is different, and the rest of the steps are identical:

[0106] The callus induction medium in this example is: on the basis of MS basic culture medium, the medium obtained by adding hydrolyzed casein, proline, PVP, vitamin C, 2,4-D, ZT, sucrose and agar , where the final concentration of hydrolyzed casein is 0.5g / L, the final concentration of proline is 0.6g / L, the final concentration of PVP is 10mg / L, the final concentration of vitamin C is 10mg / L, 2,4-D The final concentration of ZT is 2.0mg / L, the final concentration of ZT is 2.0mg / L, the final concentration of sucrose is 30g / L, the final concentration of agar is 0.8%; the pH of this medium is 5.8; The solvent is water, and the solute is shown in Table 1.

[0107] The experimental results are shown in Table 7. As can be seen from Table 7, compared ...

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Abstract

The invention discloses a method for cultivating a sweet sorghum tissue and a special culture medium thereof. The special culture medium provided by the invention is used for cultivating a sweet sorghum young ear tissue and introducing callus; based on MS basic nutrient fluid, casein hydrolysate, proline, polyvinylpyrrolidone (PVP), vitamin C, 2,4-D, ZT, cane sugar and jellies are added into the nutrient fluid to form the culture medium, wherein the final concentration of the casein hydrolysate is between 0.3 and 1g / L; the final concentration of the proline is between 0.3 and 1g / L; the final concentration of the PVP is between 150 and 200mg / L; the final concentration of the vitamin C is between 5 and 20mg / L; the final concentration of the 2,4-D is between 1.0 and 4.0mg / L; the final concentration of the ZT is between 0.1 and 4mg / L; and the final concentration of the cane sugar is between 20 and 40g / L. The method utilizes a sweet sorghum young ear as an explant to establish a tissue cultivation system and obtain a regeneration plant. By using the method and the special culture medium of the invention, a stable and high-efficient regeneration system with good repeatability can be obtained.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for tissue culture of sweet sorghum and a special culture medium thereof. Background technique [0002] Sorghum (Sorghum bicolor (L.) Moench) is the fifth most important grain in the world after wheat, rice, maize and barley. For a long time, sorghum has been widely cultivated as a multi-purpose crop in high temperature and less rainy regions in Asia, Africa and America to provide food, feed, fuel, brewing and industrial starch. Due to the exhaustion of energy resources and environmental pollution caused by the extensive use of fossil energy resources, and the global CO 2 With the huge pressure of emission reduction, the demand for biomass energy is increasing. As a variety of sorghum, sweet sorghum has become an important energy crop and is gradually attracting great attention due to its stalk sap rich in sugar, high biomass and strong stress resistance. [0003] Until n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 刘宣雨赵利铭刘树君宋松泉
Owner FUJIAN SANAN SINO SCI PHOTOBIOTECH CO LTD
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