Ph response type amphipathic stem-grafting polyphosphazenes feeding micelle and preparation method thereof

A polyphosphazene and responsive technology, which can be used in pharmaceutical formulations, antineoplastic drugs, drug combinations, etc., and can solve problems such as harsh conditions and medical application restrictions

Inactive Publication Date: 2010-07-28
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These pH-sensitive polymers used as drug carriers are almost all block copolymers, and their synthesis mainly adopts living polymerization reaction, which requires harsh conditions and often requires the use of considerable doses of heavy metals as catalysts. These problems make their medical applications greatly restricted. limit

Method used

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  • Ph response type amphipathic stem-grafting polyphosphazenes feeding micelle and preparation method thereof
  • Ph response type amphipathic stem-grafting polyphosphazenes feeding micelle and preparation method thereof
  • Ph response type amphipathic stem-grafting polyphosphazenes feeding micelle and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Example 1 Synthesis method of pH-responsive amphiphilic grafted polyphosphazene

[0089] (1) Prepare poly(dichlorophosphazene) with aluminum chloride as catalyst

[0090] Weigh 4 g of chlorophosphazene cyclic trimer purified by sublimation and 0.2 g of anhydrous aluminum trichloride into a pre-cleaned and dried polymerization tube, vacuumize and seal the tube, carry out the polymerization reaction at 250°C for 5 hours, and wait for When the viscosity of the reactant remained almost constant, the polymerization was stopped and the polymerization tube was taken out to cool. Unseal the polymerization tube, add an appropriate amount of dry toluene solution to dissolve the reactants. After dissolution, it was precipitated with petroleum ether and dried in vacuum to obtain a white elastomer, that is, poly(dichlorophosphazene).

[0091] (2) Synthesis of pH-responsive amphiphilic grafted polyphosphazene copolymers via stepwise nucleophilic substitution reactions

[0092] Dis...

Embodiment 2

[0097] Example 2 Synthesis method of pH-responsive amphiphilic grafted polyphosphazene

[0098] (1) The preparation of poly(dichlorophosphazene) is the same as in Example 1.

[0099] (2) In addition to slowly adding 2.2g NH 2 - PEG (molecular weight is 2000) and 0.2ml TEA THF solution 50ml, containing 0.5g N,N-diisopropylethylenediamine (DPA) and 0.5ml TEA THF solution 10ml and containing 0.7g 4-aminobenzoic acid Except 10 milliliters of the THF solution of ethyl ester (EAB) and 0.5ml TEA, all the other operations are all the same as the step (2) in the embodiment 1, obtain the pH response type amphiphilic grafted polyphosphazene copolymer P2, in this copolymer The mass percent content of the amino-terminated polyethylene glycol of the hydrophilic segment is 60%, the mass percent content of N-[2-(N', N'-diisopropylamino) ethyl] amino group is 15%, and the N- The mass percent content of (ethyl benzoate)-4-amino is 20%, its number average molecular weight is 5000, molecular we...

Embodiment 3

[0103] Example 3 Synthesis method of pH-responsive amphiphilic grafted polyphosphazene

[0104] (1) The preparation of poly(dichlorophosphazene) is the same as in Example 1.

[0105] (2) In addition to slowly adding 6g NH 2 50ml of THF solution of PEG (molecular weight: 2000) and 0.6ml TEA, 10ml of THF solution containing 0.8g N,N-diisopropylethylenediamine (DPA) and 0.8ml TEA, 0.8g of 4-aminobenzoic acid 10 ml THF solution of ethyl ester (EAB) and 0.7ml TEA and 10 ml THF solution of 2.0g β-hydroxyalanine ethyl ester (SEE) and 1.5ml TEA, and react at room temperature after each dropwise addition of a class of raw materials Except for 8h, all the other operations are the same as step (2) in Example 1 to obtain the pH-responsive amphiphilic grafted polyphosphazene copolymer P3, the mass percentage of the hydrophilic segment amino-terminated polyethylene glycol in the copolymer Content is 70%, the mass percent content of N-[2-(N ', N'-diisopropylamino) ethyl] amino is 10%, the ...

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Abstract

The invention discloses a pH response type amphipathic stem-grafting polyphosphazenes feeding micelle containing 70%-99.999% of pH response type amphipathic stem-grafting polyphosphazenes and 0.001%-30% of at least one hydrophobic medicament by the total weight of the medicament and the pH response type amphipathic stem-grafting polyphosphazenes, wherein the hydrophobic medicament is a hydrophobic anti-tumor medicament or a hydrophobic chemotherapeutic medicament. The micelle can be prepared by using a film hydration method or a dialysis method and can exist in a state of aqueous dispersion or frozen dry powder. The medicament of the micelle has pH response when released, the micelle can rapidly release the medicament nearby tumor tissues or in tumor cells at fixed points to reverse the multi-medicament resistance of the tumor cells, and meanwhile, the amphipathic stem-grafting polyphosphazenes has good biocompatibility and less toxicity and is suitable for being used as a targeting carrier of the anti-tumor medicament.

Description

technical field [0001] The invention relates to a drug release system, in particular to a pH-responsive amphiphilic grafted polyphosphazene administration micelle and a preparation method thereof. Background technique [0002] Tumor cell multidrug resistance (MDR) refers to the phenomenon that tumor cells develop cross-resistance to other antitumor drugs with completely different structures and mechanisms of action while developing resistance to one antitumor drug. The formation mechanism of MDR is very complicated, both with multidrug resistance associated protein (MRP), P-glycoprotein (P-glycoprotein, P-gp) on the cell membrane, lung multidrug resistance associated protein ( LRP), breast cancer resistance-associated protein (BCRP) and other membrane proteins are also related to DNA topoisomerase II (topoisomerase, Topo II), protein kinase C (proteinkinase C, PKC), glutathione (glutathione, GSH) and glutathione-s-transferase (glutathione-s-transferase, GST) and other enzym...

Claims

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Application Information

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IPC IPC(8): A61K47/34A61K9/10A61K9/19A61K45/00A61P35/00
Inventor 邱利焱郑程
Owner ZHEJIANG UNIV
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