Method for preparing Hap-PAM biological gradient compound coating by impulse hydrothermal electrophoretic polymerization method

Abstract

The invention provides a method for preparing a Hap-PAM biological gradient compound coating. The method comprises the following steps of: preparing aqueous solution of acrylamide and suspension of hydroxyapatite isopropyl alcohol respectively from hydroxyapatite, acrylamide monomers, isopropyl alcohol and the like serving as raw materials in a certain concentration ratio, performing DC electrodeposition processing on a carbon / carbon matrix in the aqueous solution of acrylamide for some time, and placing the carbon / carbon matrix in the suspension of hydroxyapatite for hydrothermal electrophoretic polymerization; and preparing the Hap-PAM gradient compound coating which has the advantages of uniform and compact surface, complete crystal orientation and close bonding of the coating and the matrix at different deposition temperatures and voltages. Due to the advantages, particularly higher bonding force, the Hap-PAM biological gradient compound coating has a quite good development prospect in clinical application.

Description

technical field

[0001] The invention relates to a preparation method of a high-strength HAp-PAM biological composite coating, in particular to a preparation method of a pulsed hydroelectrophoretic polymerization method for preparing a HAp-PAM biological gradient composite coating. The method can prepare a uniform surface, A biocomposite coating with stable structure and high bonding strength with the substrate. Background technique

[0002] In recent years, bone repair and bone substitute materials have gradually become a research hotspot in the field of biomaterials. As a bone repair and replacement material, not only biocompatibility and bioactivity must be satisfied, but also the mechanical properties of human bone. In view of this, the focus of research developed in recent years is mainly on biological coatings. The matrix material meets the requirements of the mechanical properties of biological materials, while the coating material has good biological activity. [00...

Examples

Embodiment 1

[0016] Example 1: Step 1: Dissolve 3.5g of analytically pure acrylamide monomer in water to make acrylamide aqueous solution with a concentration of 35g / L, then add 0.035g of analytically pure sodium sulfite and 0.035g of ammonium persulfate initiator respectively Fully dissolve in acrylamide aqueous solution, seal and store in a dark place to obtain solution A; Step 2: Take 3.0g of hydroxyapatite with an average particle size of 10-50nm and disperse it in 150ml of isopropanol, and then add 0.6g / L of iodine, ultrasonically dispersed for 12 hours, and aged for 8 hours to obtain B solution; Step 3: using a density of 1.8g / cm 3 The 3D C / C composite material is cut into 10mm×10mm×2mm thin slices, polished and irradiated with ultraviolet light for 20min, cleaned in distilled water, ultrasonically cleaned with absolute ethanol, dried with hot air, and fixed on the electrodeposition device and place it in the A solution by DC electrodeposition at room temperature at 80V for 5s, then ...

Embodiment 2

[0017] Example 2: Step 1: Dissolve 5g of analytically pure acrylamide monomer in water to make acrylamide aqueous solution with a concentration of 50g / L, then add 0.050g of analytically pure sodium sulfite and 0.050g of ammonium persulfate initiator to propylene respectively Fully dissolved in the amide aqueous solution, sealed and stored in a dark place to obtain solution A; Step 2: Take 2.5g of hydroxyapatite with an average particle size of 10-50nm and disperse it in 150ml of isopropanol, and then add 1.0g / L of Iodine, ultrasonically dispersed for 8 hours, and aged for 15 hours to obtain B solution; Step 3: Use a density of 1.8g / cm 3 The 3D C / C composite material is cut into 10mm×10mm×2mm thin slices, polished and irradiated with ultraviolet light for 30 minutes, cleaned in distilled water, ultrasonically cleaned with absolute ethanol, dried with hot air, and fixed on the electrodeposition device and put it in the A solution, take it out after depositing at 100V for 3s at r...

Embodiment 3

[0018] Example 3: Step 1: Dissolve 6g of analytically pure acrylamide monomer in water to make acrylamide aqueous solution with a concentration of 60g / L, then add 0.060g of analytically pure sodium sulfite and 0.060g of ammonium persulfate initiator to propylene respectively Fully dissolved in the amide aqueous solution, sealed and stored in a dark place to obtain solution A; Step 2: Take 2.0g of hydroxyapatite with an average particle size of 10-50nm and disperse it in 150ml of isopropanol, and then add 0.8g / L of Iodine, ultrasonically dispersed for 5 hours, and aged for 20 hours to obtain B solution; Step 3: Use a density of 1.8g / cm 3The 3D C / C composite material is cut into 10mm×10mm×2mm thin slices, polished and irradiated with ultraviolet light for 40min, cleaned in distilled water, ultrasonically cleaned with absolute ethanol, dried with hot air, and fixed on the electrodeposition device and place it in the A solution by direct current electrodeposition at room temperatu...

technical field

[0001] The invention relates to a preparation method of a high-strength HAp-PAM biological composite coating, in particular to a preparation method of a pulsed hydroelectrophoretic polymerization method for preparing a HAp-PAM biological gradient composite coating. The method can prepare a uniform surface, A biocomposite coating with stable structure and high bonding strength with the substrate. Background technique

[0002] In recent years, bone repair and bone substitute materials have gradually become a research hotspot in the field of biomaterials. As a bone repair and replacement material, not only biocompatibility and bioactivity must be satisfied, but also the mechanical properties of human bone. In view of this, the focus of research developed in recent years is mainly on biological coatings. The matrix material meets the requirements of the mechanical properties of biological materials, while the coating material has good biological activity. [00...

Examples

Embodiment 1

[0016] Example 1: Step 1: Dissolve 3.5g of analytically pure acrylamide monomer in water to make acrylamide aqueous solution with a concentration of 35g / L, then add 0.035g of analytically pure sodium sulfite and 0.035g of ammonium persulfate initiator respectively Fully dissolve in acrylamide aqueous solution, seal and store in a dark place to obtain solution A; Step 2: Take 3.0g of hydroxyapatite with an average particle size of 10-50nm and disperse it in 150ml of isopropanol, and then add 0.6g / L of iodine, ultrasonically dispersed for 12 hours, and aged for 8 hours to obtain B solution; Step 3: using a density of 1.8g / cm 3 The 3D C / C composite material is cut into 10mm×10mm×2mm thin slices, polished and irradiated with ultraviolet light for 20min, cleaned in distilled water, ultrasonically cleaned with absolute ethanol, dried with hot air, and fixed on the electrodeposition device and place it in the A solution by DC electrodeposition at room temperature at 80V for 5s, then ...

Embodiment 2

[0017] Example 2: Step 1: Dissolve 5g of analytically pure acrylamide monomer in water to make acrylamide aqueous solution with a concentration of 50g / L, then add 0.050g of analytically pure sodium sulfite and 0.050g of ammonium persulfate initiator to propylene respectively Fully dissolved in the amide aqueous solution, sealed and stored in a dark place to obtain solution A; Step 2: Take 2.5g of hydroxyapatite with an average particle size of 10-50nm and disperse it in 150ml of isopropanol, and then add 1.0g / L of Iodine, ultrasonically dispersed for 8 hours, and aged for 15 hours to obtain B solution; Step 3: Use a density of 1.8g / cm 3 The 3D C / C composite material is cut into 10mm×10mm×2mm thin slices, polished and irradiated with ultraviolet light for 30 minutes, cleaned in distilled water, ultrasonically cleaned with absolute ethanol, dried with hot air, and fixed on the electrodeposition device and put it in the A solution, take it out after depositing at 100V for 3s at r...

Embodiment 3

[0018] Example 3: Step 1: Dissolve 6g of analytically pure acrylamide monomer in water to make acrylamide aqueous solution with a concentration of 60g / L, then add 0.060g of analytically pure sodium sulfite and 0.060g of ammonium persulfate initiator to propylene respectively Fully dissolved in the amide aqueous solution, sealed and stored in a dark place to obtain solution A; Step 2: Take 2.0g of hydroxyapatite with an average particle size of 10-50nm and disperse it in 150ml of isopropanol, and then add 0.8g / L of Iodine, ultrasonically dispersed for 5 hours, and aged for 20 hours to obtain B solution; Step 3: Use a density of 1.8g / cm 3The 3D C / C composite material is cut into 10mm×10mm×2mm thin slices, polished and irradiated with ultraviolet light for 40min, cleaned in distilled water, ultrasonically cleaned with absolute ethanol, dried with hot air, and fixed on the electrodeposition device and place it in the A solution by direct current electrodeposition at room temperatu...

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