Preparation of TCH/HNTs/PLGA nanometer composite fibre medicine carrying system

A nanocomposite fiber and drug-loading system technology, which is applied in the field of preparation of TCH/HNTs/PLGA nanocomposite fiber drug-loading system, can solve the problems of not finding the mechanical properties of electrospun nanofiber mats, and achieve good drug sustained release effect, The effect of avoiding sudden release phenomenon and easy operation

Inactive Publication Date: 2010-11-24
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Retrieving domestic and foreign literature and patents on electrospun nanofibers shows that: no composite electrospinning of HNTs and polymers

Method used

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  • Preparation of TCH/HNTs/PLGA nanometer composite fibre medicine carrying system
  • Preparation of TCH/HNTs/PLGA nanometer composite fibre medicine carrying system
  • Preparation of TCH/HNTs/PLGA nanometer composite fibre medicine carrying system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Take 100mg of the model drug TCH and add it to 100ml of phosphate buffered saline (PBS), heat and stir at 80-90°C for about 30 minutes to dissolve to obtain the TCH solution, until the solution is clear, cool to room temperature, and set aside;

[0046] Take 100mg of HNTs and mix with the above TCH solution, put it in an ultrasonic cleaner for 50min, so that HNTs can be evenly dispersed in the TCH solution;

[0047] Place the reagent bottle containing the mixed solution of TCH and HNTs in a vacuum drying oven with the open mouth, and vacuumize for 15 minutes under the condition of 0.085MPa until there are no more bubbles on the surface of the liquid; Vacuum again in the drying oven to completely remove the air between the inner cavity of the HNTs and the HNTs, so that TCH can be adsorbed to the surface of the HNTs and / or loaded into the inner cavity;

[0048] Transfer the solution in the reagent bottle to a centrifuge tube, centrifuge at 3000rpm for 25min, take out the ...

Embodiment 2

[0050] The supernatant obtained by centrifugation in Example 1 was diluted 10 times, and the absorbance of the TCH solution at 270 nm was tested with a UV spectrophotometer. According to the concentration-absorbance relationship curve calibrated with the TCH solution, the remaining HNTs after loading the drug can be calculated. The amount of TCH in the supernatant, then, the amount of TCH loaded on the HNTs can be calculated, and the drug loading efficiency (drug loading efficiency (%)=mass of TCH on HNTs / mass of HNTs) can be calculated.

Embodiment 3

[0052] The PLGA of 1mg is dissolved in the TMF / DMF (3: 1) solvent of 4ml, is mixed with the solution that mass concentration percentage is 25%, leaves standstill overnight; Add HNTs powder into PLGA solution, ultrasonically disperse for 20min to prepare electrospinning solution, and then prepare nanofiber mat according to the conventional electrospinning method, wherein the receiving distance is 15cm, the voltage is 20kV, and the flow rate is 0.8ml / h. The composite nanofiber felt was dried in a vacuum oven for 48 hours, and then used.

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Abstract

The invention relates to a preparation of a TCH/HNTs/PLGA nanometer composite fibre medicine carrying system, comprising the following steps of: (1) taking the model medicine of tetracycline hydrochloride in phosphate buffer, heating, stirring, dissolving and cooling; (2) taking halloysite nanotubes to mix with the TCH solution for ultrasonic dispersion, placing in a vacuum drying box, vacuumizing, centrifuging, taking out the supernate, washing and precipitating to obtain HNTs carried with TCH; (3) drying, grinding and filtering the HNTs carried with TCH; and (4) dissolving PLGA in TMF/DMF solvent, standing, adding the powder obtained in the step (3) in the PLGA solution for ultrasonic dispersion to prepare electrostatic spinning solution, and then preparing the nanometer fibre felt by the regular electrostatic spinning method. The invention is simple and easy to operate; the mechanical strength of the fibre felt mixed with HNTs is obviously improved; and the dual-carrier medicine carrying system of HNTs/PLGA has favorable medicine continuous releasing effect.

Description

technical field [0001] The invention belongs to the field of preparation of TCH / PLGA nanocomposite fibers, in particular to the preparation of a TCH / HNTs / PLGA nanocomposite fiber drug-carrying system. Background technique [0002] In recent years, with the rise of nanotechnology and the emergence of various biocompatible polymer materials, nanoscale drug delivery systems based on polymer materials have attracted widespread attention. In order to change the "burst release" phenomenon of traditional pharmaceuticals and reduce the toxic and side effects of drugs on the human body, it has always been a goal of researchers to develop a drug-controlled release system with sustained release effects. [0003] Electrospinning technology is a new spinning method that uses electrostatic force to supplement or replace the mechanical force in traditional spinning methods to control fiber processing. In 1934, Formhals designed the first device for spinning by spraying polymer solution un...

Claims

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Application Information

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IPC IPC(8): A61K9/00A61K47/34A61K47/04A61K31/65A61P1/02
Inventor 史向阳齐瑞岭俞建勇
Owner DONGHUA UNIV
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