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Novel composite bacterial agent for degrading crop straw

A technology of crop straw and compound bacterial agent, applied in the direction of microorganism-based methods, microorganisms, fungi, etc., can solve the problems of low cellulose degradation rate, low lignin degradation rate, high lignin degradation rate, etc., and achieve high degradation rate , simple preparation process and wide sources

Inactive Publication Date: 2011-01-19
HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, people have begun to pay attention to the research of flora and compound bacterial agents, but many of them are limited to laboratory experiments, and in the prior art, most of them are only screened for one of lignin and cellulose to degrade straw, In straw degradation, either the cellulose degradation rate is high and the lignin degradation rate is low, or the lignin degradation rate is high and the cellulose degradation rate is low, which is far from meeting the actual needs of crop straw composting.

Method used

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  • Novel composite bacterial agent for degrading crop straw

Examples

Experimental program
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Effect test

Embodiment 1

[0029] 1. Preparation of compound bacterial agent

[0030] Cladosporium coccidioides GC2-2 was added to the strain pure culture medium (CMC-Na 10g, peptone 3.0g, KH 2 PO 4 4.0g, MgSO 4 ·7H 2 O 0.03g, water 1000ml, pH 6) Shaker culture, 30 ℃, 180r / min, time 4d, obtain Cladosporium coccidioides GC2-2 bacterium liquid.

[0031] Auricularia 916-1 and shiitake mushroom 939 were added to the pure culture medium of the strain (2% corn flour, 2% wheat bran, 1% glucose, 0.3% yeast extract, 0.1% potassium dihydrogen phosphate, 0.1% magnesium sulfate) in a shaker Cultivate at 26°C, 180r / min, for 6 days to obtain Auricularia 916-1 bacterial liquid and Lentinus edodes 939 bacterial liquid.

[0032] The above three kinds of bacterial solutions are fully mixed according to the ratio of 22% Cladosporium coccidioides GC2-2, 23% Auricularia 916-1, and 55% Lentinus edodes 939 to obtain a composite bacterial agent.

[0033] 2. Composting of straw

[0034] Crush 500kg of corn stalks into sm...

Embodiment 2

[0036] 1. Preparation of compound bacterial agent

[0037] Cladosporium coccidioides GC2-2 was added to the strain pure culture medium (CMC-Na 10g, peptone 3.0g, KH 2 PO 4 4.0g, MgSO 4 ·7H 2 O 0.03g, water 1000ml, pH 6) Shaker culture, 30 ℃, 180r / min, time 4d, obtain Cladosporium coccidioides GC2-2 bacterium liquid.

[0038] Auricularia 916-1 and shiitake mushroom 939 were added to the pure culture medium of the strain (2% corn flour, 2% wheat bran, 1% glucose, 0.3% yeast extract, 0.1% potassium dihydrogen phosphate, 0.1% magnesium sulfate) in a shaker Cultivate at 26°C, 180r / min, for 6 days to obtain Auricularia 916-1 bacterial liquid and Lentinus edodes 939 bacterial liquid.

[0039] The above three bacterial solutions are fully mixed according to the ratio of 25% Cladosporium coccidioides GC2-2, 25% Auricularia 916-1, and 50% Lentinus edodes 939 to obtain a composite bacterial agent.

[0040] 2. Composting of straw

[0041] Crush 500kg of corn stalks into small pieces...

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Abstract

The invention discloses a fungal composite bacterial agent. The fungal composite bacterium agent is prepared by the following steps of: separating and purifying soil and a plant sample to obtain different fungal strains; selecting the fungal strains with high cellulose and ligninolytic enzyme generating capacity from the fungal strains; and optimally combining and proportioning to obtain cladosporium sphaerospermum fungus, auricularia polytricha and champgnon which can effectively degrade the lignin or the cellulose of the straw. The composite bacterial agent is applied to composting of the crop straw and can compost the crop straw in 10 to 15 days without the condition of high temperature.

Description

1. Technical field [0001] The invention belongs to the technical field of microbial application, and relates to the technology of producing agricultural fertilizers by microbial composite bacterial agents, in particular to a fungal composite bacterial agent for degrading crop straws used for straw composting and returning to the field. 2. Technical Background [0002] Crop straw is a renewable biological resource rich in active ingredients and a potential non-competitive resource in crop production. More than half of the photosynthetic products of crops exist in the straw. In addition to most of the carbon elements, the organic matter contained includes cellulose, hemicellulose, lignin, protein, fat and ash, etc., and the mineral elements include nitrogen (0.5% -1.5%), phosphorus (0.1%-0.5%), potassium (0.74%-2.4%), magnesium (0.01%-0.4%), calcium (0.2%-0.5%), silicon (1%-5%) and Sulfur (0.11%-0.35%), etc., is a renewable biological resource with multiple uses. Crop straw ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/14C05F11/08C12R1/645
Inventor 谢宝恩甄静王继雯周伏忠陈国参李冠杰刘莹莹
Owner HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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