Culture medium for production of riemerella anatipestifer vaccine and preparation method thereof

Abstract

The invention discloses a culture medium for production of a riemerella anatipestifer vaccine, which comprises a basic culture medium and an additive. The basic culture medium comprises the following components in percentage: 1.5 to 1.9 percent of tryptone, 0.2 to 0.7 percent of yeast extract, 0.2 to 0.7 percent of beef extract, 0.2 to 0.7 percent of hydrolytic albumin, 0.1 to 0.4 percent of glucose, 0.5 percent of NaCl, 0.1 to 0.7 percent of monopotassium phosphate, 0.5 to 0.9 percent of Na2HPO4.12H2O, 0.1 to 0.2 percent of (NH4)2SO4 and 0.01 to 0.03 percent of NH4Cl, wherein the percentage of each component is weight / volume percentage. The additive is sterilely extracted infected-area strong cattle blood through a jugular vein blood taking or carotid artery blood bleeding method, is subjected to sterile fiber removal, is subjected to freeze thawing at the temperature of 20 DEG C below zero and at room temperature for three times, is preserved for later use at the temperature of 20 DEG C below zero, or is directly preserved at the temperature of 20 DEG C below zero after the blood is extracted and subjected to sterile fiber removal, and is subjected to freeze thawing for three times before use. The culture medium has the advantages of capacities of reducing cost, increasing nutrition, overcoming the defects of complex operation and high labor cost during culture by using live animals or embryos, and use for large-scale fermentation production of the riemerella anatipestifer vaccines.

Description

technical field [0001] The invention relates to a culture medium for Riemerella anatipestifer vaccine production and a preparation method thereof. Background technique [0002] Duck infectious serositis is a contagious disease that occurs in domestic ducks, geese, turkeys and a variety of poultry. Syndrome, duck septicemia and new duck disease, the pathogen is Riemerella anatipestifer (Riemerella anatipestifer, RA). The disease presents as acute sepsis or chronic infection. Clinically, the main manifestations are cough, panting, diarrhea, increased eye and nose secretions, ataxia, and head and neck tremor. Perihepatitis, air sacculitis, meningitis, and caseous salpingitis. The disease can occur all year round, and it is serious in winter, mainly through contaminated feed, drinking water, droplets, etc.; 1-8 week-old ducks are highly sensitive, especially ducklings 2-3 week old have a higher incidence High, up to 90%; the mortality rate is affected by many factors, and the...

AI Technical Summary

Problems solved by technology

Among the three types of culture media mentioned above, the culture of animals or live embryos is cumbersome and requires a lot of work, and cannot be used for industrialized large-scale fermentation production; The method with a high proportion of animal blood or serum is costly and there is currently no report on the effect of large-scale fermentation production in factories

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