Preparation and use of truncated Cap protein of porcine circovirus type 2

A porcine circovirus and truncated type technology, applied in the field of genetic engineering, can solve the problems of restricted expression, high price, low expression of PCV2Cap protein, etc., and achieve the effect of solving the problem of high cost
CN101948849AInactive Publication Date: 2011-01-19PU LIKE BIO ENG
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
PU LIKE BIO ENG
Publication Date
2011-01-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a vaccine of the truncated Cap protein of porcine circoviruses type 2 and a preparation method thereof. The preparation method comprises: firstly, cloning the Cap protein gene, in which 41 amino acid residues in an N terminal is removed, of the porcine circoviruses type 2 into a plasmid pKK-233-2 to obtain a recombinant expression plasmid pKK-233-2-Cap; and transforming Escherichia coli DH5 alpha by the recombinant expression plasmid to obtain recombinant genetic engineering bacteria DH5 alpha / pKK-233-2-Cap. The addition of IPTG or lactose for induction is avoided, the recombinant genetic engineering bacteria express the truncated Cap protein constitutively, the truncated Cap protein is separated and purified, and an adjuvant is added to obtain the vaccine of the truncated Cap protein of porcine circoviruses type 2. The Cap protein can also be used for preparing reagent for diagnosing related diseases caused by the porcine circoviruses type 2.
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Description

technical field

[0001] The invention relates to a genetically engineered vaccine of porcine circovirus type 2 and its preparation method, and uses truncated Cap protein as an ELISA detection antigen to identify porcine circovirus type 2 infection, especially porcine circovirus type 2 truncated type A Cap protein vaccine belongs to the field of genetic engineering. Background technique

[0002] Porcine circovirus (PCV) is the smallest known animal virus. According to the pathogenicity, antigenicity and nucleotide sequence of PCV, it is divided into two serotypes, PCV1 (porcine circovirus type 1) and PCV2 (porcine circovirus type 2). PCV1 widely exists in pigs and pig-derived cell lines, and it has been proved by animal infection experiments that PCV1 has no pathogenicity. PCV2 is associated with a variety of diseases that occur in pigs, such as multisystemic wasting syndrome in weaned piglets, porcine dermatitis nephrotic syndrome, porcine respiratory disease syndrome, and ...

Claims

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