Preparation method of surface dendritic macromolecule modified superparamagnetism ferroferric oxide nano particle
A triiron tetroxide and superparamagnetic technology, which is applied in the manufacture of nanostructures, ferrous oxides, iron oxide/iron hydroxide, etc. Large particle size, poor superparamagnetic properties, etc., to achieve the effects of good compatibility and stability, high surface polymer content, good solubility and stability
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Embodiment 1
[0032] Synthesis of superparamagnetic Fe by pyrolysis 3 o 4 The nanoparticle material is then reacted with carboxyl-bearing polyamide-amine (PAMAM) dendrimers for surface modification to generate superparamagnetic Fe with surface-modified PAMAM dendrimers 3 o 4 Nanoparticles, the size of which is 6-10nm.
[0033] Add 5.65g iron acetylacetonate (Fe(acac) 3 ), 13.6g oleic acid, 12.8g oleylamine and 120ml diphenyl ether, after mixing evenly, in N 2 Under protection, slowly heat from room temperature to 100°C, stir and react for 0.5h, then raise the temperature to 200°C for 2h, then heat to reflux temperature (about 256°C), remove heat source after reflux for 1h, and cool to room temperature naturally. After ethanol washing, strong magnet separation, redissolution, centrifugation and collection, and vacuum drying at 80°C, black solid superparamagnetic Fe was obtained. 3 o 4 Nanoparticles.
[0034] Add 0.5g Fe to a three-necked flask equipped with mechanical stirring and ref...
Embodiment 2
[0040] Synthesis of superparamagnetic Fe by pyrolysis 3 o 4 The nanoparticle material is then reacted with carboxyl-bearing polyester dendrimers for surface modification to generate superparamagnetic Fe with surface-modified dendrimers 3 o 4 Nanoparticles, the size of which is 6-10nm.
[0041] Add the superparamagnetic Fe prepared by 0.5g of above-mentioned example one step (a) in the three-neck flask that mechanical stirring, reflux condenser are housed 3 o 4 Nanoparticles, 10ml DMF, 20ml of butanone solution of 1.0g polyester dendrimer (m=2) and 20ml o-dichlorobenzene, after stirring and mixing evenly, in N 2 Under protection, the temperature was raised and kept at 70°C for 48h. Stop the reaction, naturally cool to room temperature, and separate with a magnet. The obtained black solid is dissolved in butanone and then centrifuged. After repeated washing and centrifugation for 2-3 times, vacuum-dry to obtain a superparamagnetic surface-modified polyester dendritic polyme...
Embodiment 3
[0044] Example 3 Preliminary Determination of Drug Loading
[0045] Weigh the superparamagnetic Fe of the surface-modified PAMAM dendrimer synthesized in Example 1 of 10 mg 3 o 4 Put the nanoparticles and 2.5 mg doxorubicin (Dox) into the reaction tube, add 3-4ml methanol and 60 microliters of triethylamine, stir at room temperature in the dark for 24 hours, stop stirring, and separate the upper layer with a magnet Collect, superparamagnetic Fe of the surface-modified PAMAM dendrimer synthesized in Example 1 is detected by ultraviolet-visible 3 o 4 The drug loading of nanoparticles can reach 23%.
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