Method for producing salt-free compound amino acid powder from combination of sesame seed meal and soybean meal
A compound amino acid and joint production technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of single amino acid composition, and achieve the effect of simple preparation method, improved purity, and mild conditions
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Embodiment 1
[0023] (1) Sesame meal dry powder 0.5kg, soybean meal 0.5kg, mix, grind to 40 mesh, add 3 kg of water, heat to 90°C, heat-resistant a-amylase 150 units, keep for 90min, cool to 60°C Add 100 units of glucoamylase per gram of mixture and keep it for 90 minutes. Cool to 50°C and add 200 units of cellulase per gram of mixture, keep warm for 2 hours, then cool to 40°C, add 2000 units of compound protease per gram of mixture. Based on the weight of the mixture, 0.08% of high-temperature resistant Saccharomyces cerevisiae high-activity dry yeast is added to carry out simultaneous enzymatic hydrolysis and fermentation. The composite protease described therein is composed of four kinds of proteases, neutral protease, acid protease, alkaline protease and papain, and the mixing weight ratio thereof is 1:1:1:1.
[0024] (2) Enzymolysis and fermentation for 4 days at the same time, and filtration. Obtained filtrate 3.2kg, filter residue 1.6kg. Add 20 g of activated carbon, heat to boil,...
Embodiment 2
[0027] (1) Sesame meal dry powder 0.2kg, soybean meal 0.8kg, mix, grind to 60 mesh, add 10kg of water, heat to 70°C, heat-resistant a-amylase 70 units, keep for 30min, cool to 60°C according to Add 100 units of glucoamylase per gram of mixture and keep it for 30 minutes. Cool to 50°C and add 100 units of cellulase per gram of the mixture, keep warm for 2 hours, then cool to 28°C, and add 1000 units of compound protease per gram of the mixture. Based on the weight of the mixture, 0.02% of high-temperature resistant Saccharomyces cerevisiae high-activity dry yeast is added to carry out simultaneous enzymatic hydrolysis and fermentation.
[0028] (2) Enzymolysis and fermentation for 7 days at the same time, and filtration. 9.3 kg of filtrate and 1.5 kg of filter residue were obtained. Add 30g of activated carbon, heat to boil, heat and stir to decolorize for 2h, filter, filter the cake after washing and filtering with 1kg of water, then concentrate the combined filtrate at 0.04...
Embodiment 3
[0031](1) Sesame meal dry powder 0.3kg, soybean meal 0.7kg, mix, grind to 50 mesh, add 5kg of water, heat to 80°C, heat-resistant a-amylase 120 units, keep for 90min, cool to 60°C according to Add 100 units of glucoamylase per gram of mixture and keep it for 60 minutes. Cool to 50°C and add 150 units of cellulase per gram of the mixture, keep warm for 2 hours, then cool to 35°C, and add 2000 units of compound protease per gram of the mixture. Based on the weight of the mixture, 0.06% of high-temperature resistant Saccharomyces cerevisiae high-activity dry yeast is added to carry out simultaneous enzymatic hydrolysis and fermentation.
[0032] (2) Enzymolysis and fermentation for 6 days at the same time, and filtration. Obtained filtrate 4.5kg, filter residue 1.3kg. Add 10g of activated carbon, heat to boil, heat and stir to decolorize for 4 hours, filter, and concentrate the filtrate at 0.04~0.08MPa until it is further concentrated to 27 degrees Baume, and the volume percent...
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