Method for treating methyl orange dye waste water
A dye wastewater and treatment method technology, applied in the field of applied microbiology and environmental engineering, can solve the problems of complex preparation process, poor operation stability, and secondary pollution of the environment, and achieve the effect of simple operation, small environmental impact, and good stability
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Embodiment 1
[0024] Preparation of white rot fungus spore liquid:
[0025] Pick a small amount of bacteria from the slant of the white rot fungus (Phanerochaete chrysosporium) with the preservation strain number CGMCC NO: 5.776, and inoculate it on the solid medium by streaking method. The percentages of matrix mass and dosage are: Potato Dextrose Agar 3.8%; KH 2 PO 4 0.3%; MgSO 4 ·7H 2 O 0.15%, after 5 days of cultivation at 30°C, the spore hyphae of the white rot fungus amplified in large quantities, inject a small amount of sterile water into the plate, scrape off the spore powder with the push rod method, and filter the mycelium through 4 layers of lens-cleaning paper. The obtained milky white spore suspension is the white rot fungus spore liquid, which is stored in a 4°C refrigerator for subsequent use;
[0026] The white rot fungus used in the above steps was purchased from China General Microorganism Culture Collection and Management Center, and the strain number is CGMCC NO: 5....
Embodiment 2
[0028] Preparation of bacterial cellulose membrane:
[0029] The bacterial strain number that is preserved on the slant is CGMCC NO:1.1812 Acetobacter xylinum Gluconacetobacter xylinum is inserted in 50mL seed culture medium, and the percentage that each component consumption accounts for the quality consumption of seed culture medium in the described seed culture medium is respectively: Glucose 2%; peptone 0.5%; yeast extract 0.3%; Na 2 HPO 4 12H 2 O 0.2%; KH 2 PO 4 0.1%; MgSO 4 ·7H 2 O 0.025%; citric acid 0.2%; pH5.8; cultivated on an air bath constant temperature shaker at 160rpm for 2 days to obtain an activated Acetobacter xylinum culture solution, take 10mL of this Acetobacter xylinum culture solution and insert it into 125mL fermentation medium , the percentages of the amount of each component in the fermentation medium to the quality of the fermentation medium are as follows: glucose 2%; peptone 0.5%; yeast extract 0.3%; Na 2 HPO 4 12H2 O 0.2%; KH 2 PO 4 0.1%...
Embodiment 3
[0032] Modification of bacterial cellulose membrane:
[0033] Take 20g of bacterial cellulose membrane and put it into 100mL of 1mol / L sodium hydroxide solution, then add 9mL of epichlorohydrin, react in a constant temperature water bath at 50°C for 75min, take out the membrane and wash it with acetone to remove the residues on the membrane. Small molecular organic matter; after washing with water, add 5mL of ethylenediamine and 95mL of distilled water, shake fully, heat in a constant temperature water bath at 70°C for 20min, then take out the membrane and wash it with distilled water until neutral, then use 1% by weight After washing 3 times with hydrochloric acid with low content, distilled water was washed to neutrality, then rinsed with acetone, and then vacuum-dried to constant weight. At this time, the flexibility of the film became stronger, and the surface was light yellow, smooth and shiny. Bacterial cellulose membrane after curing;
[0034] Immobilization of white r...
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