Study and application of quantum dot molecular imprinting microsphere quartz fluorescent sensor for detecting trace multicomponent food additives quickly on site

Abstract

The invention discloses a quantum dot molecular imprinting microsphere quartz fluorescent sensor for detecting multicomponent food additives simultaneously and a method for detecting the food additives by the quantum dot molecular imprinting microsphere quartz fluorescent sensor. A method for preparing a quantum dot molecular imprinting microsphere comb quartz plate comprises the following steps of: selecting functional monomers corresponding to the food additives; preparing quantum dots and preparing quantum dot molecular imprinting microspheres according to the literature; and modifying thesurfaces of different probes of the comb quartz plate with the imprinting microspheres of different food additives by utilizing layer-upon-layer accumulated surface modification technology. The method for detecting the trace multicomponent food additives simultaneously (which is shown as the figure) comprises the following steps of: immersing the modified quartz plate into simply-pulpified food solution, arranging the quartz plate on a sealing quartz vessel, and detecting the food additives in a sample. The quantum dot molecular imprinting microsphere quartz fluorescent sensor has high specificity and sensitivity, short detection time and low cost; and in a method for detecting pesticide residues by fluorescent light, the operation is simple and quick, and reactions and results are completed and recorded automatically by instruments.

Description

technical field [0001] The present invention relates to the technical field of on-site rapid high-throughput food additive detection, more specifically to the preparation of a quantum dot molecularly imprinted microsphere quartz fluorescent sensor for detecting trace food additives. The present invention also relates to the use of the quantum dot molecule Method for on-site detection of trace food additives in food, wine and beverage samples by imprinted microsphere quartz fluorescent sensor. Background technique [0002] Food additives mainly include antioxidants, colorants, color protectants, flavor enhancers, preservatives, sweeteners, emulsifiers, thickeners, etc. according to the different uses of the additives. These food additives, which are permitted by regulations and considered safe, do not guarantee absolute safety in fact, and may cause physical and health damage to some people under certain conditions. For example, aspartame is considered the ideal sweetener fo...

AI Technical Summary

Problems solved by technology

[0006] 1. Gas phase and liquid chromatography have a certain specificity and a certain ability to detect multiple components simultaneously, but their cost is too high, their sensitivity is low, and they are not suitable for large-scale rapid on-site detection;

[0007] 2. The sensitivity of paper chromatography, colorimetry, and thin-layer chromatography is low, and complete quantitative detection cannot be realized. The specificity of the method itself is poor, and false positive results are prone to occur;

[0008] 3. Although oscillopolarography and ultraviolet spectrophotometry have high sensitivity and relatively simple operation, their selectivity is poor and they cannot accurately detect the content of the analyte from complex food samples

[0009] 4. For the detection and analysis of food additives, the above methods generally have disadvantages such as low detection sensitivity, high cost, false positive results, complicated detection and sample processing process, single detection, large amount of reagents, and not suitable for on-site rapid detection. Therefore, it cannot meet the needs of actual detection

Images