Stain for producing heat-resisting and acid-resisting cellulase with high yield and new method for fermenting cellulosic ethanol

A technology of cellulosic ethanol and cellulase, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of no major breakthrough, complexity, and many unexpected factors, and achieve low production cost , Improve production efficiency and reduce production costs

Inactive Publication Date: 2011-09-07
ZHENGZHOU UNIV +4
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Problems solved by technology

Because the metabolic process of microorganisms is very complex, the current research needs to be further improved before it can play a greater role.
The other is the comprehensive biotechnology fermentation method using group fermentation, which involves the symbiosis of more than two microorganisms, and there are many unexpected factors. So far, there has been no major breakthrough

Method used

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  • Stain for producing heat-resisting and acid-resisting cellulase with high yield and new method for fermenting cellulosic ethanol

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Embodiment approach

[0021]1. Through long-term screening work, obtain Aspergillus terreus M11 with high yield of heat-resistant and acid-resistant cellulase, and the strain preservation number is CCTCC M209212. The optimal reaction temperature and pH of the produced cellulase are 60-65°C and pH 2-3, and have high thermal stability. The solid fermentation condition of optimal enzyme production fermentation is: with the bran after common autoclaving steam sterilization and 0.8% yeast extract as carbon and nitrogen source, initial pH 3, add basic salt solution [(NH 1 ) 2 SO 4 3.5 g / L, KH 2 PO 4 3 g / L, MgSO 4 .7H 2 O0.5 g / L, CaCl 2 0.5 g / L] Humidity kept at about 80%, inoculate 10 6 -10 7 spores / kg cultured at 45°C. After culturing for about 90 hours, the amount of enzyme produced reaches the maximum value, and the highest filter paper enzyme activity can reach about 240U.

[0022] 2. Comprehensive biotechnology fermentation ethanol production technology:

[0023] (1) Solid fermentation...

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Abstract

The invention relates to a microbe and application thereof, in particular to an aspergillus terreus strain M11 for high production of cellulase, and a new method for fermenting the cellulase to produce ethanol by using the strain comprehensive bioprocessing. The technology provided by the invention comprises the following steps of: obtaining the aspergillus terreus M11 for producing heat-resisting and acid-resisting cellulose with high yield, wherein the produced cellulase has the highest enzyme activity when the pH is between 2 and 3 and the temperature is between 60 DEG C and 65 DEG C; and finishing enzyme production, hydrolysis saccharification and ethanol fermentation in one system by using the strain. According to the new comprehensive bio-processing fermentation method, the preprocessed lignocellulose is directly fermented to produce the ethanol in one step, therefore the comprehensive bio-processing fermentation is realized, and the production cost is greatly saved.

Description

technical field [0001] The invention belongs to the separation and fermentation technology of microbial strains, and relates to the separation and acquisition of a novel thermoacidophilic Aspergillus terreus M11 capable of high-yielding heat-resistant and acid-resistant cellulase, and a novel one-step comprehensive biological process for fiber production based on the bacteria A new fermentation method for ethanol. Background technique [0002] Lignoplant cellulose is the most abundant and cheapest renewable resource in nature, accounting for about 20%-45% of the average dry weight of plant tissues. Cellulosic substances produced by photosynthesis in the world are as high as 1.55×10 11 tons, 89% of which have not yet been exploited by humans. Straw containing a lot of cellulose, plant decay and urban domestic waste are mainly disposed of through incineration and landfill, which not only pollutes the environment but also wastes a lot of resources. Using cellulose resources ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/10C12R1/865C12R1/66
CPCY02E50/16Y02E50/10
Inventor 翁海波孙武举王明娥关方霞
Owner ZHENGZHOU UNIV
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