Stain for producing heat-resisting and acid-resisting cellulase with high yield and new method for fermenting cellulosic ethanol

A technology of cellulosic ethanol and cellulase, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of no major breakthrough, complexity, and many unexpected factors, and achieve low production cost , Improve production efficiency and reduce production costs

Inactive Publication Date: 2011-09-07
ZHENGZHOU UNIV +4
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Problems solved by technology

Because the metabolic process of microorganisms is very complex, the current research needs to be further improved before it can play a greater role.
The other is the comprehensive biotechnolog

Method used

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  • Stain for producing heat-resisting and acid-resisting cellulase with high yield and new method for fermenting cellulosic ethanol

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Embodiment approach

[0021]1. Through long-term screening work, obtain Aspergillus terreus M11 with high yield of heat-resistant and acid-resistant cellulase, and the strain preservation number is CCTCC M209212. The optimal reaction temperature and pH of the produced cellulase are 60-65°C and pH 2-3, and have high thermal stability. The solid fermentation condition of optimal enzyme production fermentation is: with the bran after common autoclaving steam sterilization and 0.8% yeast extract as carbon and nitrogen source, initial pH 3, add basic salt solution [(NH 1 ) 2 SO 4 3.5 g / L, KH 2 PO 4 3 g / L, MgSO 4 .7H 2 O0.5 g / L, CaCl 2 0.5 g / L] Humidity kept at about 80%, inoculate 10 6 -10 7 spores / kg cultured at 45°C. After culturing for about 90 hours, the amount of enzyme produced reaches the maximum value, and the highest filter paper enzyme activity can reach about 240U.

[0022] 2. Comprehensive biotechnology fermentation ethanol production technology:

[0023] (1) Solid fermentation...

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Abstract

The invention relates to a microbe and application thereof, in particular to an aspergillus terreus strain M11 for high production of cellulase, and a new method for fermenting the cellulase to produce ethanol by using the strain comprehensive bioprocessing. The technology provided by the invention comprises the following steps of: obtaining the aspergillus terreus M11 for producing heat-resisting and acid-resisting cellulose with high yield, wherein the produced cellulase has the highest enzyme activity when the pH is between 2 and 3 and the temperature is between 60 DEG C and 65 DEG C; and finishing enzyme production, hydrolysis saccharification and ethanol fermentation in one system by using the strain. According to the new comprehensive bio-processing fermentation method, the preprocessed lignocellulose is directly fermented to produce the ethanol in one step, therefore the comprehensive bio-processing fermentation is realized, and the production cost is greatly saved.

Description

technical field [0001] The invention belongs to the separation and fermentation technology of microbial strains, and relates to the separation and acquisition of a novel thermoacidophilic Aspergillus terreus M11 capable of high-yielding heat-resistant and acid-resistant cellulase, and a novel one-step comprehensive biological process for fiber production based on the bacteria A new fermentation method for ethanol. Background technique [0002] Lignoplant cellulose is the most abundant and cheapest renewable resource in nature, accounting for about 20%-45% of the average dry weight of plant tissues. Cellulosic substances produced by photosynthesis in the world are as high as 1.55×10 11 tons, 89% of which have not yet been exploited by humans. Straw containing a lot of cellulose, plant decay and urban domestic waste are mainly disposed of through incineration and landfill, which not only pollutes the environment but also wastes a lot of resources. Using cellulose resources ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/10C12R1/865C12R1/66
CPCY02E50/16Y02E50/10
Inventor 翁海波孙武举王明娥关方霞
Owner ZHENGZHOU UNIV
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