Agricultural biological activity and application of metabolite of Platycladus orientalis endophytic fungus

A technology of endophytic fungi and metabolites, applied in the fields of chemicals, applications, biocides, etc. for biological control, which can solve the problems that have not been reported in the literature and limit the large-scale production and use of botanical pesticides.

Inactive Publication Date: 2011-10-05
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as we all know, plant resources are one of the main factors limiting the large-scale production and use of botanical pesticides
Based on the long-term co-evolution of the host plant and its endophytic microorganisms, the applicant has the same or similar secondary metabolite biosynthesis pathway "endosymbiosis theory", isolated and screened from Arborvitae, which has a significant inhibitory effect on various agricultural diseases, insect pests and weeds endophytic fungi, related research has not been reported in the literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Typical isolation and culture of endophytic fungi from orientalis

[0017] The stems and leaves of arborvitae were collected from the campus of Qingdao Agricultural University, washed with tap water and dried, soaked in 75% ethanol for 5 minutes, soaked in 0.1% mercury liter for 2 minutes, and disinfected the surface, and finally rinsed with sterile water for 5 times. Blot dry with sterile filter paper for later use. Stem tissues after surface disinfection were directly inoculated on the surface of PDA medium, placed in a (27±1)°C incubator for cultivation, and stem tissues without any bacteria growing on the surface were selected.

[0018] In a sterile state, the above-mentioned sterile stem tissue was cut into 0.2cm×0.2cm fragments, inserted into fresh PDA medium, and cultured in a constant temperature incubator at (27±1)°C. When mycelium grows obviously around the sample, use the tip picking method of mycelium. According to the color, shape and sequence o...

Embodiment 2

[0021] Example 2: Screening of Typical Activity (Bactericidal) of Endophytic Bacteria of Arborvitae

[0022] Take 1mL of the fermentation supernatant of each of the above strains in a sterile petri dish, and quickly mix it with 9mL of PDA medium cooled to 50°C. After cooling and solidifying, put a test bacteria with a diameter of 4mm on each medium plate. Cakes were cultured in a 28°C incubator. The sterile water treatment was used as the control and repeated three times. The colony diameter was determined by the cross method, and the inhibition rate was calculated by the following formula:

[0023] Mycelial growth inhibition rate=(control colony diameter-treatment colony diameter)×100% / (control colony diameter-4)

[0024] The results show that: there are 21 strains with antagonistic effect on at least one plant pathogenic fungus (accounting for 19.1% of the total number of isolates), and the endophytic fungi numbered G21, J77, J86, Y51, Y57, Y127 have an antagonistic effect...

Embodiment 3

[0025] Example 3: Preparation of a typical preparation of endophytic bacteria from Thuja arborvitae

[0026] The product contains 2% of the metabolites of J77 strains, 3% of white carbon black is added as an adsorbent, and 1% of Nongru 0203-B and 3% of sodium lignosulfonate are added as auxiliary materials, and finally supplemented with light calcium carbonate 100%, after mixing, it is made into wettable powder by jet milling.

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Abstract

The invention (the title is agricultural biological activity and application of metabolite of Platycladus orientalis endophytic fungus) relates to a Platycladus orientalis endophytic fungus, a separation cultivation method thereof, and an inhibition effect of a metabolite of the fungus on main agricultural pests and weeds. The fungus, which is separated from Platycladus orientalis, has a good bacteriostatic activity on various agricultural plant pathogenic fungi such as Valsa mali, Elsinoe ampelina, Verticillium dahliae, Fusarium oxysporum and the like, and has a certain inhibition effect on various weeds such as Amaranthus retroflexus, wild oat and the like and various pests.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to the agricultural biological activity and application of endophytic fungus metabolites of arborvitae. Background technique [0002] Platycladus orientalis (L.) Franco belongs to Cupressaceae (Cupressaceae) Platycladus Spach. It is a unique tree species in my country and one of the commonly used tree species in urban greening, especially in northern cities. Arborvitae leaves are fragrant, bitter and slightly pungent, and have the effects of cooling blood and stopping bleeding, clearing the lungs and relieving cough. The extract of oriental cypress leaves has different degrees of antibacterial activity against Staphylococcus aureus and Escherichia coli, and its rhizosphere soil extract has inhibitory effects on the germination and seedling growth of radish and other plant seeds. [0003] Qingdao Agricultural University began to study the pesticide activity ...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P3/00A01P1/00A01P13/00A01P7/00
Inventor 郝双红王现坤魏艳
Owner QINGDAO AGRI UNIV
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