Method for extracting corylus heterophylla leaf flavone
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A technology of flavonoids and extraction methods of hazel flavonoids, which is applied in the field of flavonoids extraction, can solve the problems that the extraction method of hazel flavones has not been reported and restricts the application of hazel flavones, and achieves short extraction time, low organic solvent residues, and strong application prospects Effect
Inactive Publication Date: 2012-12-05
HEILONGJIANG UNIV
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[0004] Hazel flavone is a biologically active substance rich in hazel. It is composed of a variety of flavonoids characterized by quercetin, kaempferol and its glycosides. They have a variety of physiological functions and can effectively prevent cancer. Antioxidant, blood lipid lowering, preventive effect on cardiovascular diseases, based on the unique physiological effects of hazelin, its application value is very high, but there is no report on the extraction method of hazelin
At present, there is no production and sales of hazelaflavone in the market, thus restricting the application of hazelaflavone
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specific Embodiment approach 1
[0016] Specific embodiment one: the present embodiment is the extraction method of flavonoids of hazel leaves, which is achieved through the following steps:
[0017] 1. Pick the leaves of Corylus plants without disease, rinse them with clean water, and then dry them at 30°C to 100°C until the water content in the leaves is less than 3%, and then crush the dried leaves to particle size. The diameter is 20-120 mesh, and the leaf powder of Corylus is obtained;
[0018] 2. Mix the Corylus leaf powder obtained in step 1 with the immobilized enzyme to obtain the extract, wherein the mass ratio of the Corylus leaf powder and the immobilized enzyme is 0.1~100:1, and the enzyme used in the immobilized enzyme One or more of cellulase, xylanase, pectinase, hemicellulase and helicase;
[0019] 3. Put the extraction material in step 2 into the extraction kettle, and perform supercritical carbon dioxide extraction to obtain the extraction mixture. The extraction conditions are as follows:...
specific Embodiment approach 2
[0029] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the Corylus plant described in step 1 is selected from flat hazel, hairy hazel, thorn hazel, Chuan hazel, Hua hazel, velvet hazel, Yunnan hazel, Weixi One of Hazel, Tibetan Hazel, Short-handled River Hazel and European Hazel, or a hybrid between two of them. Other steps and parameters are the same as those in Embodiment 1.
specific Embodiment approach 3
[0030] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in step 1, the leaves are dried at 40°C to 80°C until the mass percentage of moisture in the leaves is less than 3%. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.
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Abstract
The invention discloses a method for extracting corylus heterophylla leaf flavone, relating to a method for extracting flavone substances. The method has the advantages of small residual quantity of organic solvents, environmental friendliness, high yield and low impurity content of the obtained corylus heterophylla leaf flavone. The method comprises the following step of: performing supercritical carbon dioxide extraction on a extraction material obtained by mixing corylus plant leaf powder with immobilized enzyme, wherein an entrainer can be added in the supercritical carbon dioxide extraction process, and the entrainer is a mixture of one or more of methanol, ethanol and acetone. Compared with the conventional widely-applied solvent extraction technology, the method has the advantages that: a flavone compound is extracted from corylus heterophylla leaves by combining immobilized enzyme enzymolysis with a supercritical CO2 leaching technology, the residual quantity of organic solvents is small, the immobilized enzyme can be recycled, the extraction time is short, the yield of corylus heterophylla leaf flavone is as high as 0.57-5.12 percent, a large quantity of impurities can beremoved, the corylus heterophylla leaf flavone can be well concentrated and enriched, and strong application prospect is achieved.
Description
technical field [0001] The invention relates to a method for extracting flavonoids, in particular to a method for extracting flavonoids from hazel leaves. Background technique [0002] The active ingredients in plant materials exist in cells surrounded by cell walls and protoplasts. The outer walls are composed of cellulose, hemicellulose, pectin, lignin and other substances. There are also proteins, lipid compounds, Sugars and other substances form obstacles to the extraction and purification of natural compounds such as flavonoids. In recent years, researchers have successively adopted liquid-phase enzymes (such as aqueous phase enzymes) to degrade cell walls, intercellular junctions and some components that interfere with extraction, which can not only shorten the extraction time, but also increase the yield of active components. In recent years, based on the research and development of aqueous enzyme catalysis, a new and distinctive non-aqueous medium, namely supercriti...
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