A kit for in-situ non-destructive detection of tumors and its preparation method

A kit and organic solvent technology, applied in the fields of pharmacy, analysis and detection, and medicine, can solve the problems of incomplete application of hydrophobic quantum dot encapsulation, poor dispersion performance of hydrophobic quantum dots, complicated operation steps, etc., so as to increase the photostability. and biocompatibility, improved stability and biocompatibility, good biocompatibility

Inactive Publication Date: 2011-12-14
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

CN101629076A discloses a method for preparing silica-coated fluorescent quantum dot nanoparticles, which can effectively solve the problem of poor dispersion of hydrophobic quantum dots in the aqueous phase, making it more suitable for biological applications
However, several methods for encapsulating quantum dots disclosed in this patent are cumbersome, difficult to control, and not completely suitable for encapsulating hydrophobic quantum dots.

Method used

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  • A kit for in-situ non-destructive detection of tumors and its preparation method
  • A kit for in-situ non-destructive detection of tumors and its preparation method
  • A kit for in-situ non-destructive detection of tumors and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of Fluorescent Probes Embedded in Chitosan Micelles with Hydrophobic Quantum Dots

[0029] 1. Preparation of N-succinyl-N'-octyl chitosan micelles:

[0030] (1). Preparation of N-succinoyl chitosan micelles (SC)

[0031] Chitosan 1g was dissolved in 4.8% succinic acid (20ml) solution, was diluted with methanol (80ml) solution dropwise, mechanically stirred at room temperature, was added dropwise with succinic anhydride solution (succinic anhydride: 2-amino-β- The molar ratio of 1,4-glucan was 5:1, and the stirring was continued at room temperature for 48 hours, the pH of the system was adjusted to 7 with 5% sodium hydroxide solution, the precipitate was collected by centrifugation, dialyzed and freeze-dried.

[0032] (2). Preparation of N-succinyl-N'-octyl chitosan (SOC)

[0033]1g of N-succinyl chitosan was dissolved in 1% acetic acid (50ml) solution, and octanal was added dropwise under stirring (octylal: 2-amino-β-1,4-glucan mole ratio was 2:1 ), after...

Embodiment 2

[0045] Preparation of Fluorescent Probes Embedded in Chitosan Micelles with Hydrophobic Quantum Dots

[0046] 1. Preparation of N-octyl-O-sulfate chitosan (OSC) micelles:

[0047] (1). Preparation of N-octyl chitosan micelles (OC)

[0048] 1g of chitosan was dissolved in 1% acetic acid (50ml) solution, and octanal was added dropwise under stirring (octylaldehyde: 2-amino-β-1, 4-glucan mole was 2: 1), and continued stirring After 4 hours, the pH was adjusted to 4 with a small amount of sodium hydroxide, and sodium borohydride solution was added dropwise and stirring was continued for 12 hours. Filtrate, adjust the pH to 7 with sodium hydroxide, and a large amount of precipitation occurs. The precipitate was collected by centrifugation, dissolved in water, dialyzed, and freeze-dried.

[0049] (2). Preparation of N-octyl-O-sulfate chitosan (OSC)

[0050] 1 g of OC was dissolved in DMF (40 ml) and stirred overnight. Add sulfuric acid ester dropwise in the DMF (40ml) solution,...

Embodiment 3

[0058] Preparation of Fluorescent Probes Embedded in Chitosan Micelles with Hydrophobic Quantum Dots

[0059] 1. Preparation of N-succinyl-N'-palmitoyl chitosan (SPC) micelles:

[0060] (1). Preparation of N-succinoyl chitosan micelles (SC)

[0061] Chitosan 1g was dissolved in 4.8% succinic acid (20ml) solution, was diluted with methanol (80ml) solution dropwise, mechanically stirred at room temperature, was added dropwise with succinic anhydride solution (succinic anhydride: 2-amino-β- 1,4-dextran molar ratio is 5:1), stirring was continued at room temperature for 48 hours, 5% sodium hydroxide solution was used to adjust the pH of the system to 7, the precipitate was collected by centrifugation, dialyzed and then freeze-dried.

[0062] (2). Preparation of N-succinoyl-N'-palmitoyl chitosan (SPC)

[0063] 1g N-succinyl chitosan was dissolved in 100mL DMSO solution, and was added dropwise under stirring conditions with palmitic anhydride (palmitic anhydride: 2-amino-β-1,4-glu...

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Abstract

The invention relates to the technical field of medicine, pharmaceuticals and analysis detection, particularly relates to an in-situ tumor nondestructive detection kit and a preparation method thereof. The in-situ tumor nondestructive detection kit is characterized in that the kit comprises chitosan micelle phosphoric acid buffer solution, wherein hydrophobic lead sulfide quantum dots are embedded inside the chitosan micelles. The in-situ tumor nondestructive detection kit provided by the invention is simple for operation and tumor-targeting, has the characteristics of high light quantum yield, strong light stability, good biocompatibility and increased solubility of hydrophobic quantum dots, and can conduct nondestructive in-situ real-time monitoring, so that the kit is more suitable for in-situ imaging and detection of tumors.

Description

technical field [0001] The invention relates to the technical fields of medicine, pharmacy and analysis and detection, in particular to a kit for in-situ non-destructive detection of tumors and a preparation method thereof, which is suitable for fluorescence detection of non-destructive in-situ tumors. Background technique [0002] The diagnosis of tumors and related diseases has so far mainly relied on some in vitro detection methods and imaging techniques such as ultrasound, X-ray, CT, nuclear magnetic resonance imaging and PET. The diagnosis of cancer is currently mainly based on the morphology and other macroscopic characteristics of tumor tissue or diseased cells, which is often an invasive and time-consuming process that is not suitable for early diagnosis of cancer. Therefore, the early diagnosis of cancer is still an unprecedented challenge faced by the medical field so far. Optical imaging methods, especially fluorescent imaging methods, have the advantages of bein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00C08L5/08C08K3/30
Inventor 顾月清朱红艳曹洁
Owner CHINA PHARM UNIV
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