A method for the detection of aromatic amines in mainstream cigarette smoke by gas chromatography-tandem mass spectrometry

A cigarette mainstream smoke, tandem mass spectrometry technology, applied in measurement devices, instruments, scientific instruments and other directions, can solve problems such as tedious and time-consuming, and achieve the effect of simplifying sample pretreatment steps, saving concentration time, and simplifying sample pretreatment.

Active Publication Date: 2011-12-14
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for detecting aromatic amines in cigarette mainstream smoke by gas chromatography-tandem mass spectrometry in order to solve the problem of cumbersome and time-consuming pretreatment in the determination method of aromatic amine compounds in the prior art

Method used

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  • A method for the detection of aromatic amines in mainstream cigarette smoke by gas chromatography-tandem mass spectrometry
  • A method for the detection of aromatic amines in mainstream cigarette smoke by gas chromatography-tandem mass spectrometry
  • A method for the detection of aromatic amines in mainstream cigarette smoke by gas chromatography-tandem mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The balanced and screened cigarettes were smoked according to the standard conditions stipulated in YC / T29-1996, and the particulate matter in the mainstream smoke of 20 cigarettes was captured with a Cambridge filter.

[0036] Fold and put the Cambridge filter disc for trapping particulate matter into the Erlenmeyer flask, pipette 100mL of 5% HCl solution, add 60 ng of internal standard, ultrasonically extract at room temperature for 30 min, and let it stand for 5 min; take 50 mL of the extract to In a 250 mL separatory funnel, wash with 50 mL×3 dichloromethane, discard the organic phase, adjust the pH of the aqueous phase to 12–13 with sodium hydroxide, then extract with 50 mL×3 n-hexane, and add 15 g of Dry over anhydrous sodium sulfate overnight. Add 80 μL of trimethylamine hydrochloride and 40 μL of pentafluoropropionic anhydride to derivatize the solution for not less than 40 min, take 50 mL of the solution on a rotary evaporator, concentrate it to 2 mL by rotary ...

Embodiment 2

[0042] The Kentucky reference cigarette 2R5F was smoked according to the standard conditions stipulated in YC / T 29-1996, and the particulate matter of 20 cigarettes was collected by the Cambridge filter. Place the filter disc in the conical flask, add a certain amount of D7-1-aminonaphthalene and D9-4-aminobiphenyl mixed solution on the filter disc with a micro-sampling needle, and then carry out the sample according to the steps described in Example 1 Purification treatment analysis; 5 average experiments were carried out, and the results showed that the relative standard deviation of each compound was between 2.36% and 6.98%, indicating that the method had good reproducibility and stability.

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Abstract

The invention discloses a method for detecting aromatic amine in cigarette mainstream smoke by using a gas chromatography-tandem mass spectrometry. The method is characterized by comprising the following steps of: catching aromatic amine compounds in a particulate matter in the cigarette mainstream smoke by using a Cambridge filter; extracting by using hydrochloric acid solution, washing by usingdichloromethane, reducing by using sodium hydroxide and then extracting by using normal hexane, and performing derivatization by using pentafluoropropionic anhydride and trimethylamine hydrochloride;and concentrating and detecting by using the gas chromatography-tandem mass spectrometer (GC-MS/MS) so as to measure the content of the main aromatic amine compounds in the cigarette mainstream smoke. Compared with the conventional method for analyzing the aromatic amine in the cigarette mainstream smoke, the analysis and measurement method has the advantage of simplifying the pretreatment of a sample along with higher sensitivity, high experimental repetitiveness and recovery rate and applicability to analysis of a large batch of samples.

Description

[0001] technical field [0002] The invention belongs to the technical field of smoke analysis and measurement, and in particular relates to a gas chromatography-tandem mass spectrometry method for detecting aromatic amines in cigarette mainstream smoke. [0003] Background technique [0004] Aromatic amines and their derivatives have mutagenic factors, which can cause poisoning through inhalation, ingestion or absorption through the skin, and can cause damage to the human blood system through the formation of methemoglobin, and can also directly act on liver cells, causing toxic liver disease. Some aromatic amine derivatives also have carcinogenic and mutagenic effects, even at low concentrations, they have carcinogenic activity on animals and humans. The HOFFMANN list contains 4 kinds of aromatic amine compounds, namely 1-aminonaphthalene (1-amn), 2-aminonaphthalene (2-amn), 3-aminobiphenyl (3-amb), 4-aminobiphenyl ( 4-amb). Therefore, the determination of aromatic amin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/72G01N30/06
Inventor 余晶晶王昇夏巧玲谢复炜张晓兵刘惠民
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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