A kind of modification method of extracellular matrix

A technology of extracellular matrix and cells, applied in medical science, prosthesis, etc., can solve the problem of high scalability and achieve the effect of controllable mechanical properties and enhanced extracellular matrix

Active Publication Date: 2011-12-21
GUANGZHOU SUN SHING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For the enhancement, functionalization and staining of extracellular matrix, there is currently no simple, controllable, low toxicity and high scalability method

Method used

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  • A kind of modification method of extracellular matrix
  • A kind of modification method of extracellular matrix

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Genipin was configured as an aqueous solution with a concentration of 50 mg / mL, and 100 mg / mL of double-terminal amino polyethylene glycol (H 2 N-(PEG) 2000 -NH 2 ) aqueous solution was mixed in equal volumes, and then 1 μg / mL of nerve growth factor (NGF) solution was added, human sciatic nerve extracellular matrix was soaked in it, and the decellularized nerve matrix with enhanced cross-linking was obtained at 37°C for 12 hours. Tissue engineered scaffolds for peripheral nerve bridging. After testing, the tensile strength of human sciatic nerve extracellular matrix is ​​changed from the original 14.5±0.3 N / mm 2 Enhanced to 23.2±0.8 N / mm 2 , to achieve the predetermined goal, and the mechanical strength of the sciatic nerve (22.8±1.2 N / mm 2 ) are similar; on the other hand, NGF can be covalently linked to the extracellular matrix, and the immobilization efficiency reaches 65%.

Embodiment 2

[0023] Configure genipin as an aqueous solution with a concentration of 100 mg / mL, mix it with an equal volume of 100 mg / mL ethylenediamine aqueous solution, soak the extracellular matrix of porcine cornea in it, and shake it at a constant temperature of 37°C for 5 hours to obtain dyed blue The corneal extracellular matrix is ​​used for cosmetic surgery after corneal disease.

Embodiment 3

[0025] Prepare genipin hydroxyethyl ester as a dimethyl sulfoxide (DMSO) solution with a concentration of 1 mg / mL, mix it with 10 mg / mL diamine-based silicone oil (brand C845) in DMSO in equal volumes, and take human sciatic nerve extracellular The matrix was soaked in it, and reacted at a constant temperature of 37 ° C for 10 hours to obtain a decellularized nerve matrix with enhanced cross-linking, which was used as a tissue engineering scaffold for peripheral nerve bridging.

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Abstract

The invention discloses a method for modifying an extracellular matrix. Genipin or a derivate thereof is used as a cross-linking agent, and at least one of biological functionalization agent, pigment and chain extender, and the extracellular matrix are subjected to contact reaction. The method can realize the enhancement of the extracellular matrix and / or dyeing or bionic functionalization, the Genipin or the derivate thereof is used as the cross-linking agent, the chain extender, the biological functionalization agent and the like are introduced, and the aims of the controllable mechanical property of the modified extracellular matrix are fulfilled by controlling reaction conditions.

Description

Technical field [0001] The invention involves a modification method of an extracellular matrix. Background technique [0002] Extraceral matrix is one of the basic components of the tissue.Its ingredients are mainly three categories: ① glycosaminoglycan, protein glycogen (Proteoglycan); ② structural protein; ③ sticky protein.Extraceral matrix plays a physical role such as connection, support, water preservation, pressure -resistant, and protection in the tissue, and can conduct comprehensive biological regulation of the basic life activities of cells.For example, to provide cells with support and fixing, regulating communication between cells, and regulating dynamic behavior of cells. [0003] The tissue engineering uses stents and seed cells, and the complex formed in vitro is used to build internal transplantation to replace and repair damaged tissue organs.The performance of the bracket is directly related to the effect of repair.The bracket performance includes many aspects, ...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/24A61L27/20
Inventor 全大萍曾晨光朱继翔强娜
Owner GUANGZHOU SUN SHING BIOTECH CO LTD
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