Method for producing ethanol or acetone and butanol by taking lignocellulose as raw material
A technology for acetone and raw materials, which is applied in the field of producing ethanol or acetone butanol, can solve the problems of high investment cost, long enzymatic hydrolysis time and large area, and achieve the effects of low energy consumption and high hydrolysis efficiency
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Embodiment 1
[0036] The corncobs were crushed into 1mm powder with a pulverizer, washed with water, and then dried overnight at 60°C. Mix 500g of corncob powder below 1-2mm with 10L of water, and place it for anaerobic fermentation at 10°C for 1 day. When the pH drops to 4.5, adjust the wet disc grinder——Japan MKZA10 ultrafine particle melting machine (Mashiko Sangyo Co., Ltd., Japan) The gap between the two upper and lower disc grinding discs is 1 micron to the appropriate gap, mix the feed liquid refrigerated at 4°C, add it to the ultra-fine melting machine, grind for 10-15 laps, and the particle size is 2 μm Between, the soluble sugar content is 0.385g / g corn cob, wherein the glucose content is about 65%, and the xylose content is about 20%. Add the pretreatment solution to the compound cellulase in an amount of 5%. The hydrolysis conditions are: 10.0mL pH4.5, 50°C, 200rpm rotary shaking for 48h. The soluble sugar content is 0.637g / g corn cob, among which the xylose content can reach ...
Embodiment 2
[0038] Corn cobs were pulverized into 2mm powder with a pulverizer. Mix 500g of corncob powder below 2mm with 5L of water, place it at 4°C for 1 day, and place it for anaerobic fermentation at 4°C for 2 days, and when the pH drops to 5.0, adjust the wet disc grinder——Japan MKZA10 ultrafine particle fusion Crusher (Mashiko Sangyo Co., Ltd., Japan) the gap between the 2 upper and lower disc grinding discs to a suitable gap of 2 microns, after grinding for 15 circles, add 10FPU / g Acremonium composite cellulase and 0.4% β- Xylosidase; after mixing, pre-hydrolyze at 50°C for 1 hour, add 0.1% yeast powder, keep the temperature at 37°C after mixing, insert acetone-butanol-producing microorganism Clostridium beijerinckii ATCC55025 at an inoculum size of 5%, and ferment 72h, butanol content is 7.8g / L, acetone content is 4.1g / L.
Embodiment 3
[0040] Grind the bran into 1mm powder with a pulverizer, mix 500g of the feed liquid with 5L of water, and place it for anaerobic fermentation at 4°C for 2 days. When the pH drops to 4.8, adjust the wet disc grinder——Japan MKZA10 The gap between the two upper and lower discs of the particle melting machine (Mashiko Sangyo Co., Ltd., Japan) to a suitable gap of 10 microns, mix the anaerobic feed liquid at 4°C, add it to the ultra-fine melting machine, and grind for 10 laps , the soluble sugar content is 0.151g / g bran. Add 6.4 FPU / g Acremonium cellulase (compound enzyme with endoenzyme and exoenzyme) and 40 μL / g β-xylosidase to the pretreatment liquid at an additive amount of 5% for hydrolysis for 48 hours. The soluble sugar content is 0.531g / g bran. Add 0.1% of yeast powder to the mixed material liquid after pretreatment and enzymolysis of the above bran, and add 0.1% yeast powder, pH 5.5 to 7.0, insert acetone butanol production microorganism Clostridium beijerinckii ATCC5502...
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