Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing D-lactic acid by using fiber hydrolysate

A technology of hydrolyzate and lactic acid, applied in the field of microbial fermentation, can solve the problems of D-lactic acid production technology to be broken through, long process route, low purity and the like, and achieves the effects of low cost, easy availability of raw materials, and simple and convenient fermentation process

Active Publication Date: 2012-01-25
ANHUI BBCA FERMENTATION TECH ENG RES
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] With the deepening of research, the use of D-lactic acid continues to expand, and the market demand will also continue to increase. However, the industrial production of D-lactic acid is still in a blank or at a standstill, which has a lot to do with the D-lactic acid production technology. big relationship
[0005] At present, most of the research directions in China are directly using glucose and corn saccharification liquid. Although there are researches on the use of fiber sugar in foreign countries, the process route is long, the acid production is low, and the purity is low.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 D-lactic acid produced by fermentation of Lactobacillus sporogenes Y2-8

[0022] Plate medium: glucose 10%, yeast extract 0.3%, anhydrous sodium acetate 0.2%, anhydrous magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.2%, agar 2%, water balance, pH7.0;

[0023] Seed medium: corncob hydrolyzate (calculated as glucose) 6%, yeast extract 1%, peptone 1%, bran 0.5%, anhydrous magnesium sulfate 0.05%, ferrous sulfate 0.002%, manganese sulfate 0.001%, calcium carbonate 2%, water balance, pH7.0;

[0024] Fermentation medium: corn cob hydrolyzate (calculated as glucose) 8%, yeast extract 2%, corn steep liquor 2%, anhydrous magnesium sulfate 0.02%, ferrous sulfate 0.001%, manganese sulfate 0.001%, calcium carbonate 3%, water Balance, pH 7.0.

[0025] Wherein, the preparation of corncob hydrolyzate is as follows: corncob is treated by acid method first and then enzymatically hydrolyzed, the enzyme used in enzymolysis is cellulase, the amount of enzyme added is...

Embodiment 2

[0028] Example 2 Production of D-lactic acid by fermentation of Lactobacillus sporogenes Y2-8

[0029] Plate medium: glucose 10%, yeast extract 0.3%, anhydrous sodium acetate 0.2%, anhydrous magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.2%, agar 2%, water balance, pH7.0;

[0030] Seed medium: straw hydrolyzate (calculated as glucose) 6%, yeast extract 1%, peptone 1.2%, bran 0.6%, anhydrous magnesium sulfate 0.05%, ferrous sulfate 0.002%, manganese sulfate 0.001%, calcium carbonate 2 %, water balance, pH7.00;

[0031] Fermentation medium: straw hydrolyzate (calculated as glucose) 7%, yeast extract 2%, corn steep liquor 3%, anhydrous magnesium sulfate 0.02%, ferrous sulfate 0.001%, manganese sulfate 0.001%, calcium carbonate 3%, water Quantity, pH7.0.

[0032] Among them, the preparation of the straw hydrolyzate is as follows: the straw is first treated by alkali method and then enzymatically hydrolyzed. The enzyme used in the enzymatic hydrolysis is cellulase. li...

Embodiment 3

[0035] Example 3 Production of D-lactic acid by fermentation of Lactobacillus sporogenes Y2-8

[0036] Plate medium: glucose 10%, yeast extract 0.3%, anhydrous sodium acetate 0.2%, anhydrous magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.2%, agar 2%, water balance, pH7.0;

[0037] Seed medium: straw steam explosion hydrolyzate (calculated as glucose) 6%, yeast extract 1.2%, peptone 1.0%, bran 0.6%, anhydrous magnesium sulfate 0.05%, ferrous sulfate 0.002%, manganese sulfate 0.001%, carbonate Calcium 2%, water balance, pH7.00;

[0038] Fermentation medium: straw steam explosion hydrolyzate (calculated as glucose) 8%, yeast extract 1%, corn steep liquor 4%, anhydrous magnesium sulfate 0.015%, ferrous sulfate 0.001%, manganese sulfate 0.001%, calcium carbonate 3%, Water balance, pH7.0.

[0039] Among them, the preparation of the straw steam explosion hydrolyzate is as follows: the straw is treated by steam explosion first and then enzymatically hydrolyzed, the enzyme...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for producing D-lactic acid in a fermentation mode by using hydrolysate of a cellulose raw material such as corn cob, straw or the like. The method comprises the following steps of: pre-treating the cellulose raw material such as corn cob, straw or the like to obtain hydrolysate using glucose as a main component, adding a proper amount of auxiliary materials such as corn steep liquor, ammonium sulfate, potassium dihydrogen phosphate and the like, adding microbes, performing fermentation, and thus obtaining the D-lactic acid with high optical purity. The method is facile in raw materials, low in cost and simple and convenient in fermentation, achieves relatively high acid production capacity on the basis of ensuring high optical purity of the product, and accords with the sustainable development principle.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for producing D-lactic acid using fiber hydrolyzate. Background technique [0002] As the main polysaccharide product of plant photosynthesis, cellulose is the most abundant renewable natural resource on earth. It is estimated that the energy contained in cellulose on the earth is approximately equivalent to the energy contained in 640 billion tons of petroleum, and the renewability of cellulose is incomparable to mineral energy such as petroleum. [0003] In recent years, with the in-depth research on new biological materials, especially the in-depth research on polylactic acid, a biodegradable material obtained from the polymerization of lactic acid, the application prospects and demands of D-lactic acid are becoming more and more extensive. For example, by adding D-lactic acid in different proportions to the polymer of L-lactic acid, the degradation rate of the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12R1/225C12R1/07
Inventor 李荣杰穆晓玲李维理戴嘉何冰芳柏中中
Owner ANHUI BBCA FERMENTATION TECH ENG RES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com