Method for preparing fibroin nanoparticles taking polyvinyl alcohol as stabilizer

A technology of silk fibroin and nanoparticles, which is applied in the field of preparation of silk fibroin nanoparticles, can solve the problems of difficulty in large-scale production, low yield, and need for special equipment, and achieve low pollution, low energy consumption, and simple preparation process Effect

Inactive Publication Date: 2012-02-08
SUZHOU SIMEITE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of this method is that the size of the prepared nanoparticles is uniform, and there is no need to use a large amount of toxic organic solvents. The disadvantage is that special equipment is required, the yield is low, and it is difficult to produce on a large scale

Method used

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  • Method for preparing fibroin nanoparticles taking polyvinyl alcohol as stabilizer
  • Method for preparing fibroin nanoparticles taking polyvinyl alcohol as stabilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1) The silk fibroin protein extracted from silk is made into a silk fibroin protein solution with a weight volume percentage of 5%;

[0044] 2) Add a polyvinyl alcohol solution with a weight ratio of 1:1 to the silk fibroin solution to obtain a blend solution, and the polyvinyl alcohol solution has an average molecular weight of 30,000 Daltons , made by dissolving polyvinyl alcohol with a degree of hydrolysis of 98% in water;

[0045] 3) Add the above blend solution dropwise at a rate of 0.5 ml / min into the methanol solution stirred at a speed of 400 rpm with a volume ratio of 1:4 to the blend solution, and then continue stirring at a speed of 100 rpm for 1 hour to obtain Uniform silk fibroin nanoparticle solution;

[0046] 4) further purifying and enriching the uniform silk fibroin nanoparticle solution obtained above by ammonium sulfate salting out precipitation method or natural volatilization and centrifugation to obtain uniform silk fibroin nanoparticles with a si...

Embodiment 2

[0057] 1) The silk fibroin protein extracted from silk is made into a silk fibroin protein solution with a weight volume percentage of 8%;

[0058] 2) Add a polyvinyl alcohol solution with a weight ratio of 1:5 to the silk fibroin solution to obtain a blend solution, and the polyvinyl alcohol solution has an average molecular weight of 180,000 Daltons , the degree of hydrolysis is 87% polyvinyl alcohol dissolved in water;

[0059] 3) Add the above blend solution dropwise at a rate of 1 ml / min into ethanol stirred at a speed of 600 rpm with a volume ratio of 1:10 to the blend solution, and then continue to stir at a speed of 200 rpm for 6 hours to obtain a uniform The silk fibroin nanoparticle solution;

[0060] 4) further purifying and enriching the uniform silk fibroin nanoparticle solution obtained above by ammonium sulfate salting out precipitation method or natural volatilization and centrifugation to obtain uniform silk fibroin nanoparticles with a size of 200 nanometers...

Embodiment 3

[0071] 1) The silk fibroin protein extracted from silk is made into a silk fibroin protein solution with a weight volume percentage of 7%;

[0072] 2) Add a polyvinyl alcohol solution with a weight ratio of 1:4 to the silk fibroin solution to obtain a blend solution, and the polyvinyl alcohol solution has an average molecular weight of 100,000 Daltons , made by dissolving polyvinyl alcohol with a degree of hydrolysis of 89% in water;

[0073] 3) Add the above-mentioned blend solution dropwise at a rate of 0.8 ml / min to methanol stirred at a speed of 500 rpm with a volume ratio of 1:7 to the blend solution, and then continue to stir at a speed of 150 rpm for 4 hours to obtain a uniform The silk fibroin nanoparticle solution;

[0074] 4) further purifying and enriching the homogeneous silk fibroin nanoparticle solution obtained above by the ammonium sulfate salting out precipitation method or natural volatilization and centrifugation to obtain uniform silk fibroin nanoparticles...

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Abstract

The invention relates to a method for preparing fibroin nanoparticles taking polyvinyl alcohol as a stabilizer. The method comprises the following process steps of: (1) preparing fibroin extracted from silk into 5-8 percent by weight in volume of fibroin solution; (2) adding a polyvinyl alcohol solution into the fibroin solution to obtain a blended solution; (3) dropwise adding the blended solution into a water-soluble organic solvent which can be used for inducing beta-folding of the fibroin, and stirring continually for 1-6 hours to obtain a fibroin nanoparticle solution; (4) further purifying and enriching the fibroin nanoparticle solution to obtain uniform fibroin nanoparticles; and (5) storing the obtained fibroin nanoparticles. According to the preparation method, fibroin nanoparticles with uniform and stable particle sizes and high yield can be prepared; and the fibroin nanoparticles are suitable for large-scale production and are widely applied in a plurality of fields such as medicament release, patellofemoral joint biological lubricants, face-lifting, beauty treatment, tissue repair and the like.

Description

technical field [0001] The invention relates to a method for preparing silk fibroin nanoparticles with polyvinyl alcohol as a stabilizer, and belongs to the field of new biological materials. Background technique [0002] Silk fiber derived from silkworm cocoons has been used clinically for decades as surgical sutures. The regenerated silk fibroin (Fibroin) solution extracted from silkworm cocoons can be prepared into various forms of biomaterials without adding auxiliary agents and organic solvents, such as porous sponge-like scaffolds, films, gels, etc. Glue, micro and nano particles, etc. Compared with traditional biomaterials such as collagen and polyester copolymers, biomaterials made of silk fibroin have high mechanical strength, controllable degradation rate, good biocompatibility, low immunogenicity and inflammatory response, and are safe and reliable. And other advantages, so it is being widely researched and applied in the field of tissue engineering such as bone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08L29/04C08J3/14A61K8/64A61K47/42A61L27/22A61L31/04A61Q19/00
Inventor 张凤莲
Owner SUZHOU SIMEITE BIOTECH CO LTD
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