Preparation method for high water-holding capacity and water-insolubility corn dietary fiber

A water-insoluble, dietary fiber technology, applied in the fields of food preparation, food science, application, etc., can solve the problems of low water-holding capacity of water-insoluble dietary fiber and insufficient physiological effect, and achieves low cost, convenient operation, good effect

Inactive Publication Date: 2012-02-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The water-insoluble dietary fiber derived from corn bran is not high in water holding capacity, and its physiological effects cannot be fully exerted in the application

Method used

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  • Preparation method for high water-holding capacity and water-insolubility corn dietary fiber
  • Preparation method for high water-holding capacity and water-insolubility corn dietary fiber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] a: Preparation of non-water-soluble corn fiber

[0039] Firstly, the corn seed coats are screened, washed and dried. Use a pulverizer to pulverize and pass through a 100-mesh sieve. The fat content of corn husks is only about 4%, and the fat is removed by soaking in 6# solvent oil to obtain degreased corn husk powder and dried; the dried degreased corn husk powder and water are in a mass ratio of 1:10 Mix and stir to make a slurry, adjust pH=7.5 with dilute alkali; then add 1398 neutral protease, the enzyme amount is 2000u / gDS, enzymolyze at 45°C for 60min, then adjust pH=6 with dilute hydrochloric acid, add 0.2% (w / wDS) high-temperature-resistant a-amylase, hydrolyze at 100°C for 60 minutes, then cool down to 60°C to inactivate the enzyme to obtain a two-step enzymatic hydrolysis slurry; filter the enzymatic hydrolysis slurry, and add water to the obtained solid to make a slurry to adjust the slurry pH = 6.5 and add 0.1% (w / wDS) glucoamylase, continue hydrolysis at 5...

Embodiment 2

[0049] Take 100 g of corn seed coat powder that has been screened, dried and crushed through a 100-mesh sieve, dissolved in 900 mL of 6# solvent oil, soaked at room temperature for 5 hours, filtered to recover the solvent, and dried to obtain degreased corn hull powder; Add defatted corn husk powder into 1500mL water and make a slurry, adjust pH=7.5 with dilute sodium hydroxide solution, add 1398 neutral protease 300000u, enzymolyze at 45°C for 70min and inactivate the enzyme activity; then adjust pH with dilute hydrochloric acid =6.2, add 0.05g of high temperature resistant a-amylase, hydrolyze in boiling water bath for 80min; then cool down to 58℃, adjust pH=5.5, add 0.03g of glucoamylase, keep 58℃ for 180min, then heat to 95℃ The enzymatic activity was inactivated for 15 minutes to obtain a two-step enzymatic hydrolysis slurry. After cooling down, the enzymatic hydrolysis slurry was filtered, and the filter cake was washed with water and dried to obtain 91.3 g of intermediat...

Embodiment 3

[0052] Take 80g of corn seed coat powder that has been screened, dried and crushed through a 150-mesh sieve, dissolved in 1000mL6# solvent oil, soaked at room temperature for 4h, filtered to recover the solvent, and dried to obtain degreased corn hull powder; Add fat corn bran powder into 1300mL water and make a slurry, adjust pH=7.3 with dilute sodium hydroxide solution, add 1398 neutral protease 300000u, enzymolyze at 46°C for 90min and inactivate the enzyme activity, then adjust pH=7.3 with dilute hydrochloric acid After 6.1, add 0.05g of high temperature resistant a-amylase, hydrolyze in boiling water bath for 100min; then cool down to 56℃, add 0.02g of glucoamylase and adjust pH=5.8, keep 56℃ for 200min, then heat to 95℃ Inactivate the enzyme for 20 minutes to obtain the enzymatic slurry, lower the temperature, then filter the enzymatic slurry, wash the filter cake with water and filter to obtain a wet solid;

[0053] Disperse the above wet solid in water and stir to form...

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Abstract

The present invention provides a preparation method for high water-holding capacity and water-insolubility corn dietary fiber. The method is a multi-enzyme grading enzymolysis method, and comprises that: adopting processes of neutral protease enzymolysis, high temperature resistance-alpha amylase enzymolysis and glucoamylase combination enzyme enzymolysis; sequentially removing starch, greases, proteins and other impurities from corn bran to obtain pure water-insoluble corn fiber; adopting cellulase to carry out enzymolysis and modification to increase the water-holding capacity of the corn fiber to obtain the high water-holding capacity and water-insolubility corn dietary fiber, wherein the water-holding capacity of the high water-holding capacity and water-insolubility corn dietary fiber is changed into 8.83 g/gDS from the water-holding capacity of the corn bran of 3.44 g/gDS, the improvement range is 157%. The full- enzymolysis method adopted in the invention has characteristics ofrapidness, high performance and no chemical contamination, the equipment is simple, and the method is the potential modified method for the corn dietary fiber.

Description

technical field [0001] The invention relates to the preparation of water-insoluble dietary fiber, in particular to a preparation method of high-water-holding water-insoluble corn dietary fiber. Background technique [0002] Corn bran is a by-product produced in the production process of corn starch, and the quantity is very large. If it is not used rationally, it will not only waste resources, but also may cause environmental pollution. my country has very rich resources of corn husks, a by-product of corn processing. Using corn husks to prepare high-activity dietary fiber can reduce waste of resources, protect the environment, and improve the comprehensive development and utilization of by-products. [0003] The main components of corn bran are polysaccharides, hemicellulose, protein and water-insoluble fiber. It has high fiber content and is a source of dietary fiber with high quality and low price. The measured data of the main components of corn bran are shown in Table ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/308
Inventor 钟振声连小燕蔡佳振
Owner SOUTH CHINA UNIV OF TECH
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