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Preparation method for cowherb seed flavonoid glycoside monomer

A technology for producing flavonoid glycoside monomers and Wangbuliuxin, which is applied in the field of preparation of chemical monomers of traditional Chinese medicine, can solve the problems of difficult to obtain high-purity monomers, difficult silica gel column chromatography separation and crystallization purification, and achieve simple procedures , easy to control, and less environmental pollution

Inactive Publication Date: 2012-04-04
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After repeated practice, the flavonoid glycosides of wangbuliuxing are very water-soluble, and it is not easy to be separated by silica gel column chromatography and purified by crystallization, so it is difficult to obtain its high-purity monomer

Method used

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  • Preparation method for cowherb seed flavonoid glycoside monomer
  • Preparation method for cowherb seed flavonoid glycoside monomer
  • Preparation method for cowherb seed flavonoid glycoside monomer

Examples

Experimental program
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Effect test

Embodiment 1

[0024] A preparation method of wangbuliuxing flavonoid glycoside monomer is characterized in that it is carried out according to the following process steps:

[0025] A. Raw material extraction: Grind 5kg of wangbuliuxing medicinal material into coarse powder, add 50L of water, heat and boil for 2 hours, filter; add 50L of water to the filter residue, boil for 2 hours in the same way, filter; add 50L of filter residue again Water, boil for 2 hours in the same way, filter, combine the filtrate, and let it cool to room temperature;

[0026] B. Macroporous resin enrichment: Weigh 2kg AB-8 macroporous adsorption resin, fill it with a 15cm diameter glass column to form a resin column, and the volume of the resin bed is about 6L; pass the cooled filtrate of step A through the activated macroporous resin at a speed of 4BV. Pore ​​adsorption resin column for adsorption, then use 18L water and 12L volume fraction 30% ethanol solution to elute the impurities, then use 12L volume fracti...

Embodiment 2

[0032] A preparation method of wangbuliuxing flavonoid glycoside monomer is characterized in that it is carried out according to the following process steps:

[0033] A. Raw material extraction: Grind 10kg of wangbuliuxing medicinal material into coarse powder, add 80L of water, heat and boil for 2 hours, filter; add 80L of water to the filter residue, boil for 2 hours in the same way, filter; add 80L to the filter residue again Water, boil for 2 hours in the same way, filter, combine the filtrate, and let it cool to room temperature;

[0034] B. Macroporous resin enrichment: Weigh 4kg AB-8 macroporous adsorption resin, fill it with a 20cm diameter glass column to form a resin column, and the volume of the resin bed is about 12L; pass the cooled extract through the activated macropore at a speed of 3BV The adsorption resin column is used for adsorption, and then the impurities are eluted with 36L water and 24L 30% ethanol solution by volume fraction, and then the target subst...

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Abstract

The invention relates to a method for quickly separating a high-purity cowherb seed flavonoid glycoside monomer from a Chinese medicinal material cowherb seed and belongs to the technical field of Chinese medicines. The method comprises the following process steps of: A, extracting raw materials, namely poaching; B, enriching by using macroporous resin; C, performing high-efficiency preparation and liquid phase separation, wherein C18 fillers are adopted, the detection wavelength is 280 nm and the mobile phase is a 30 volume percent methanol solution; and D, recovering products, namely enriching a preparation solution by using the macroporous resin, desorbing by using ethanol, concentrating and drying to obtain the cowherb seed flavonoid glycoside monomer with the purity of over 99 percent. The method is simple and quick, low in solvent consumption, high in yield, and economic and environment-friendly. A large number of products can be prepared by the method at one time. The method has high economic value and academic value.

Description

technical field [0001] The invention relates to a preparation method of a chemical monomer of traditional Chinese medicine, which belongs to the technical field of traditional Chinese medicine. Specifically, the invention relates to a method for rapidly separating high-purity flavonoid glycoside monomers of blalicius from Chinese medicinal material blalicius. Background technique [0002] Vaccariae Semen (Vaccariae Semen), a Chinese herbal medicine, is the dried and mature seed of Vaccaria segetalis (Neck.) Garcke, a plant of the genus Vaccaria Medic. in the family Caryophyllaceae. Breast swelling, diuretic effect of treating stranguria. For amenorrhea, dysmenorrhea, milk retention, mastitis swelling and pain, gonorrhea astringent pain. It is recorded in the "Chinese Pharmacopoeia" of the past, and it is a good medicine for promoting lactation. [0003] Vaccarin, the chemical name is 4H-1-Benzopyran-4-one, 6-(2-O-α-L-arabinopyranosyl-β-D-glucopyranosyl)-2-[4-(β -D-glucopy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 夏柯文焕松白兰辉郭建华刘丁
Owner CHENGDU PUSH BIOLOGICAL TECH
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