Colloidal gold test paper for rapidly detecting antibody of porcine reproductive and respiratory syndrome virus

A technology for respiratory syndrome and respiratory syndrome, applied in the field of colloidal gold rapid diagnostic test strips for porcine reproductive and respiratory syndrome virus antibodies, to achieve the effects of improved sensitivity and specificity, convenient storage, and simple operation
CN102495216AInactive Publication Date: 2012-06-13JILIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JILIN UNIV
Publication Date
2012-06-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to colloidal gold test paper for rapidly detecting an antibody of a porcine reproductive and respiratory syndrome virus (PRRSV). The test paper is characterized in that: the test paper is composed of a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad which are sequentially attached to a nonabsorbent supporting sheet, wherein the combination pad is covered by PRRSVNsp9 proteins marked by colloidal gold; and the nitrocellulose membrane is respectively covered by a detection line T line composed of staphylococcus proteins A (SPA) and a quality control line C line composed of PRRSVNsp9 monoclonal antibodies 2D6. According to the invention, by utilizing an immunological principle that antigens and antibodies can be specially combined, the antigen marked by the colloidal gold is combined with the corresponding antibody and the conjugate of the antigen and the antibody is combined with the SPA to form an antigen-antibody-SPA conjugate, so as to form a color reaction on the nitrocellulose membrane (NC membrane) to be observed by naked eyes. The test paper provided by the invention has the characteristics of simplicity, fastness, sensitivity, good specificity and the like. And the price is low, so that the test paper is applicable to basically or clinically detecting the antibody of the porcine reproductive and respiratory syndrome virus. The test paper has the obvious advantages of strong specificity, high flexibility, simplicity in operation and fastness in diagnosis, and can be used for rapidly diagnosing the porcine reproductive and respiratory syndrome virus.
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Description

technical field

[0001] The invention relates to a colloidal gold rapid diagnostic test strip for porcine reproductive and respiratory syndrome virus antibody, which belongs to a new animal diagnostic reagent and is mainly used for diagnosing animals infected by porcine reproductive and respiratory syndrome virus; it is applied in the field of animal husbandry and veterinary medicine . Background technique

[0002] Porcine reproductive and respiratory syndrome (PRRS) is caused by porcine reproductive and respiratory syndrome virus (PRRSV) infection. Respiratory diseases of pigs. PRRS is a new infectious disease that broke out in the late 1980s. It first appeared in North America in 1987. In the following years, it quickly became popular in many countries in Europe and the United States, and then spread to Asia. In some countries, it has become a worldwide pandemic, causing huge economic losses to the global pig industry. The disease broke out in my country at the end of 19...

Claims

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