Method for producing virus-free potato breeder's stock

[0021] Example 1: Comparative experiment-application comparison between the method of the present invention and the traditional method

[0022] In the autumn of 2007, 5 treatments of vermiculite, perlite, vermiculite+perlite, river sand, nutrient soil+river sand+vermiculite were used as cultivation seedbeds. The thickness of each treatment was 18cm, and treatment 5 was nutrient soil 10cm+river. Sand 5cm + vermiculite 3cm, nutrient soil is vegetable garden soil, add 40g potassium sulfate compound fertilizer, 30g ammonium phosphate, 10g urea, 10g calcium magnesium phosphate fertilizer, and 150g decomposed tungku per square meter and mix well. All treatment varieties are Chuanyuzao, planted with 10m test tube seedlings according to the spacing of 5cmx7cm. 2. Repeat 3 times. Before harvest, take samples and send them to the Plant Quarantine Station of the Sichuan Provincial Department of Agriculture for testing without the six viruses of PVX, PVY, PLRV, PVS, PVM, and PVA. The test results are as follows.

[0023] The production of virus-free potato original seeds produced by different cultivation substrates and the cost of substrate

[0024]

[0025] Note: The increase in yield, the increase in yield, and the decrease in cost are all mixed layered three-dimensional cultivation methods of nutrient soil + river sand + vermiculite compared with other treatments.

[0026] Among various treatments, treatment 5, which is the mixed layered three-dimensional cultivation method, has the best effect and the highest yield, with an average harvest of 718 original seeds/m 2 , Total 2168.3g/m 2 Compared with treatment 1, the grains increased by 67% and the yield increased by 134.5%; compared with treatment 2, the increase in yield was the highest, increasing by 234% and 514.9% respectively; the size of individual potato pieces was also significantly increased, with an average single-grain weight of 3.02g, which was higher than treatment 1. The average single-grain weight was 2.15g and the weight gain was 0.87g, and the single-grain weight gain reached 40.5%; compared with treatment 4, the average single-grain weight was 1.54g and 1.48g was increased, and the weight gain reached 96.1%. And the cost of the substrate is greatly reduced. Compared with the treatments 1, 2, and 3, the cost of the substrate is reduced by 58.3%, 46.4%, and 53.1%, respectively.

[0027] The test results show that the method of processing 5 production original seeds has a significant increase in yield compared with other current main production methods. Its output, including quantity and weight, has shown a substantial increase in yield, and the size of seed potatoes has also been greatly increased; Under the condition that the cost of the substrate is greatly reduced, a significant increase in the production output per unit area of ​​the original seed and a significant increase in the weight of the single seed are realized.

[0028] Example 2:

[0029] In the autumn of 2009, the mixed three-dimensional layered cultivation method of treatment 5 was adopted. Firstly, 50g potassium sulfate compound fertilizer, 30g ammonium phosphate, 10g urea, 10g calcium magnesium phosphate fertilizer, and 200g decomposed tungs were evenly sprayed per square meter on the vegetable garden soil (thickness 12cm). After covering the river sand with a thickness of 5cm, mix well, and then spread vermiculite 3cm on top, and then plant and manage the test tube seedlings according to the technical requirements. The cultivated variety is Qinyu 30, and the planting area is 96m. 2 , A total of 207kg of original seeds were harvested, 74,000 grains, and the average production of original seeds was 2156.3g/m 2 ,771 grains/m 2. Before harvesting, an expert team sent by the Sichuan Provincial Department of Agriculture will sample the plants on site, and then the Sichuan Plant Quarantine Station will conduct DAS-ELISA virus testing in accordance with the requirements of the national standard, and detect 6 viruses (PVX, PVY, PLRV, PVS, PVM) , PVA), the results do not contain the detection virus, and the pass rate is 100%.

[0030] Example 3:

[0031] In the spring of 2010, the treatment 5 mixed three-dimensional cultivation method was adopted in all 10 simple greenhouses. The thickness of the seedling border was 25cm and the nutrient soil was 15cm (per square meter, 60g potassium sulfate compound fertilizer, 30g ammonium phosphate, 15g urea, calcium and magnesium Phosphate fertilizer 10g, decomposed tungku 200g), river sand 7cm, vermiculite 3cm, others are operated in accordance with concise technical requirements, the cultivar is Bashu No. 10, and the planting area is 1200m 2. Before harvest, plant sampling and DAS-ELISA virus detection were performed by the Plant Quarantine Station of the Sichuan Provincial Department of Agriculture. Result 1200m 2 A total of 2,517kg of the original seed of virus-free potato was harvested, with 903,600 seeds, and the average yield was 2097.5g/m 2 ,753 grains/m 2. Virus test results: all do not contain detected viruses, and the pass rate is 100%.