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Method for producing virus-free potato breeder's stock

A technology of potato and original seed, which is applied in the field of producing original seed of virus-free potato, can solve the problems of small number of tubers per plant, insufficient nutrient supply, and high requirements for the reproduction and production conditions of original seed in the next season, so as to increase the production quantity and single grain weight, promote the improvement of production level, and overcome the effect of production bottleneck restriction

Inactive Publication Date: 2012-06-20
达州市农业科学研究所
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AI Technical Summary

Problems solved by technology

The soilless culture medium overcomes the shortcomings of loose soil and insufficient ventilation, and effectively improves the survival rate of test-tube seedlings. However, the soilless culture medium itself basically has no nutrition or low fertilizer efficiency. It mainly relies on nutrient solution spraying, and the nutrient supply is insufficient. The number of tubers per plant is small, and the single grain is small. The general output in my country is 300-500 grains / m 2 , the average single grain weight is 2-2.5g
At the same time, it is very costly to use soilless culture substrates such as vermiculite, peat, perlite, etc. and to spray with nutrient solution, generally 10000m 2 , the cultivation medium needs about 200,000 yuan. At the same time, the individual of the original seed is small, so that the reproduction and production conditions of the original seed in the next season are high, and the yield is low, which further increases the cost of seed potato breeding, which limits the seed of virus-free potato. Potato breeding and large-scale application

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  • Method for producing virus-free potato breeder's stock

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Embodiment 1

[0021] Embodiment 1: comparative experiment-the inventive method and traditional method application contrast

[0022] In the autumn of 2007, 5 treatments of vermiculite, perlite, vermiculite + perlite, river sand, nutrient soil + river sand + vermiculite were used as cultivation seedbeds. The thickness of each treatment was 18cm, and treatment 5 was 10cm of nutrient soil + river Sand 5cm + vermiculite 3cm, nutrient soil is vegetable garden soil, add 40g of potassium sulfate compound fertilizer, 30g of ammonium phosphate, 10g of urea, 10g of calcium magnesium phosphate fertilizer, 150g of decomposed tung tree per square meter and mix well. Each treatment variety is Chuanyuzao, and the test tube seedlings are planted 10m according to the row spacing of 5cmx7cm. 2 . 3 repetitions. Before harvesting, samples were sent to the Plant Quarantine Station of the Sichuan Provincial Department of Agriculture to test for the absence of six viruses: PVX, PVY, PLRV, PVS, PVM, and PVA. The...

Embodiment 2

[0029] In the autumn of 2009, the mixed three-dimensional layered cultivation method of treatment 5 was adopted. On the vegetable garden soil (thickness 12cm), 50g of potassium sulfate compound fertilizer, 30g of ammonium phosphate, 10g of urea, 10g of calcium magnesium phosphate fertilizer, and 200g of decomposed Tongku were evenly spread per square meter. , cover the river sand with a thickness of 5cm, mix it evenly, and spread vermiculite 3cm on top, and then plant and manage the test tube seedlings according to the technical requirements. The cultivated variety is Qinyu 30, and the planting area is 96m 2 , a total of 207kg of original seeds, 74,000 grains were harvested, and the average production of original seeds was 2156.3g / m 2 , 771 grains / m 2 . Before harvesting, the expert group sent by the Sichuan Provincial Department of Agriculture conducted on-site sampling of the plants, and then the Sichuan Provincial Plant Quarantine Station conducted DAS-ELISA virus detectio...

Embodiment 3

[0031] In the spring of 2010, in 10 simple and simple greenhouses, all adopted the method of processing 5 mixed three-dimensional divisions. The thickness of the seedling bed was 25cm, and the nutrient soil was 15cm (60g of potassium sulfate compound fertilizer per square meter, 30g of ammonium phosphate, 15g of urea, calcium and magnesium Phosphate fertilizer 10g, decomposed tung tree 200g), river sand 7cm, vermiculite 3cm, and others are operated according to the concise technical requirements. The cultivated variety is Bashu No. 10, and the planting area is 1200m 2 . Plant sampling and DAS-ELISA virus detection were carried out by the Plant Quarantine Station of Sichuan Provincial Department of Agriculture before harvest. Result 1200m 2 A total of 2,517kg of original virus-free potato seeds, 903,600 grains, and an average yield of 2,097.5g / m were harvested 2 , 753 grains / m 2 . Virus detection results: none of the products contain any detected virus, and the pass rate is...

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Abstract

The invention relates to a method for producing virus-free potato breeder's stock. The method comprises the step of: planting virus-free potato test tube seedlings in a medium composed of nutrient soil at the lower layer, river sand at the middle layer and vermiculite at the upper layer so as to carry out mixed three-dimensional layered planting. The method has the characteristics that: the production cost of the potato breeder's stock is greatly reduced, and the unit area producing number of seeds and the weight of single grain are greatly improved at the same time, so that the production bottle-neck restriction of the potato breeder's stock is overcome, and the breeding and popularization and application of the virus-free potato are accelerated, thus elevation of the production level of potato and rapid development of the potato industry are promoted.

Description

(1) Technical field [0001] The invention relates to a method for producing the original seed of virus-free potato. (2) Background technology [0002] An important factor affecting potato yield is potato viral degradation. Potatoes have an average yield reduction of 30%-80% after virus infection. The use of biotechnology to produce virus-free seed potatoes is the most effective way to prevent and control viral diseases. In the breeding and production of virus-free seed potatoes, the original seed production is the bottleneck. The low production yield, poor efficiency and high cost of original seed production are the key factors restricting the popularization and application of virus-free potatoes. The original seed of virus-free potato is produced and bred in the greenhouse in the insect-proof net room by using the virus-free test tube seedlings. The virus-free test-tube seedlings are produced under conditions of constant temperature, high humidity, weak light (light), air...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01H4/00
Inventor 胡振兴赵思毅张玲李薇杨小丽杜仕惠刘晓英贾荟芹
Owner 达州市农业科学研究所
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