Phosvitin-sugar polymer emulsifier and preparation method thereof
A technology of phosvitin and sugar polymer, which is applied in the field of emulsifiers, can solve the problems of lack of practical application value, etc., and achieve the effects of improving emulsifying activity and emulsifying stability, increasing grafting reaction speed, and high yield
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Embodiment 1
[0016] (1) Preparation of phosvitin stock solution: Weigh phosvitin, add it to pH6.5, 0.01M phosphate buffer solution, stir at room temperature for 1 hour, and obtain a concentration of 5% ( w / v) phosvitin stock solution.
[0017] (2) Preparation of dextran stock solution: Weigh dextran with an average molecular weight of 10KDa, add it to distilled water, stir at room temperature for 0.5h, and obtain a concentration of 2% (w / v) dextran after fully dissolving. Sugar stock solution.
[0018] (3) Preparation of phosvitin and dextran mixture: according to the mass ratio of phosvitin and dextran of 1:4, use 0.01M phosphate buffer to control the pH of the system to 7.0. The two stock solutions were mixed and stirred at room temperature for 2 h to fully mix.
[0019] (4) Stirring and heat treatment: Stir the mixed solution on a magnetic stirrer with a rotation speed of 2500 rpm, and at the same time, heat the mixed solution, the temperature is controlled at 110° C., and the treatme...
Embodiment 2
[0023] Determination of emulsifying activity
[0024] The copolymer emulsifier obtained in Example 1 was dispersed in a phosphate buffer solution of pH 7.0 and 0.01M at a concentration of 0.5% (w / v), and rapeseed oil was added according to the oil-water ratio of 1:3. Use a dispersing homogenizer to homogenize at high speed for 1 min to prepare an emulsion. Immediately draw 50μL from the bottom of the emulsion, quickly disperse in 5mL, 0.1wt% sodium dodecyl sulfate solution, and measure the absorbance A at a wavelength of 500nm 0 . Emulsifying activity calculation formula: EAI = (4.606 × A 0 × dilution) / [ C × (1-Φ) × 10 4 ], C is the protein concentration, Φ is the oil content in the emulsion, Φ=0.25.
[0025] Determination of emulsion stability
[0026] After the prepared emulsion was allowed to stand for 30 min, 50 μL was sucked from the bottom, quickly dispersed in 5 mL of 0.1 wt % sodium dodecyl sulfate solution, and the absorbance A30 was measured at a wavelength ...
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