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Preparation method of low-fluorine Euphasia superb protein base stock

An Antarctic krill and protein-based technology, which is applied in the field of Antarctic krill protein base material preparation, can solve the problems of lack of Antarctic krill protein processing and preparation methods, Antarctic krill transportation and storage difficulties, and achieve transportation cost saving and autolysis. Strong ability and the effect of increasing the yield

Active Publication Date: 2012-07-04
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To solve the problem of Antarctic krill transportation and storage difficulties, the best way is to quickly process Antarctic krill to prepare protein after harvesting, but there is still no on-board processing and preparation method for Antarctic krill protein

Method used

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preparation example Construction

[0022] The invention discloses a method for on-board processing and preparation of a low-fluorine Antarctic krill protein base material, which includes the following steps in sequence:

[0023] 1. Low-temperature autolysis: Drain the fresh and live Antarctic krill and directly homogenize them, and irradiate them with ultraviolet rays with a power of 20-40 watts, a wavelength of 200-300 nanometers, and a distance of 0.5-1 meter for 5-10 minutes, and then put The homogenate is placed at the ambient temperature of the fishing area and autolyzed for 0.1 to 1 hour. This step takes advantage of the strong autolysis ability of fresh and live Antarctic krill to increase the self-dissolution of structural proteins and increase the yield of water-soluble proteins.

[0024] 2. Protein extraction: Add 1 to 4 times the volume of 0-4°C water to the homogenate after low-temperature autolysis, mix well, stir and extract at 0-4°C for 5-15 minutes, and centrifuge to obtain First upper liquid p...

Embodiment 1

[0033]Embodiment 1: The present invention uses Antarctic krill as a raw material and adopts modern protein extraction and separation technology to prepare a low-fluorine Antarctic krill protein base material onboard processing and preparation technology. Drain the fresh and live Antarctic krill after catching, mix with 2-4 times the volume of 0-4°C water and homogenate, and irradiate with ultraviolet rays with a power of 20-40 watts, a wavelength of 200-300 nanometers, and a distance of 0.5-1 meter After processing for 5 to 10 minutes, the homogenate is placed at the ambient temperature of the fishing area for autolysis for 0.1 to 1 hour. Add calcium chloride to the homogenate to make the final concentration 0.01% to 0.03%, adjust the pH of the homogenate to 2 to 3.5 with 1 to 6M hydrochloric acid or sodium hydroxide, stir and extract at 0 to 4°C for 5 ~ 15 minutes, centrifuge to obtain liquid phase A and precipitate A; add 2 to 4 times the volume of 0 ~ 4 ℃ water to precipita...

Embodiment 2

[0034] Example 2: Drain the fresh and live Antarctic krill after catching, mix with 2 times the volume of 0-4°C water and homogenate, and irradiate with ultraviolet rays with a power of 40 watts, a wavelength of 200 nanometers, and a distance of 1 meter for 10 minutes. Afterwards, the homogenate was placed at ambient temperature in the fishing area for autolysis for 1 hour. Add calcium chloride to the homogenate to make the final concentration 0.03%, adjust the pH of the homogenate to 3.5 with 6M hydrochloric acid or sodium hydroxide, stir and extract at 0-4°C for 15 minutes, and centrifuge to obtain the liquid phase A and Precipitation A; Add 4 times the volume of 0-4°C water to Precipitation A, stir and mix, add calcium chloride to the mixed solution to make the final concentration 0.03%, adjust the pH of the mixed solution with 6M hydrochloric acid or sodium hydroxide value is 3.5, stirring and leaching for 15 minutes at 0-4°C, centrifuged to obtain liquid phase B and preci...

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Abstract

The invention discloses a preparation method of low-fluorine Euphasia superb protein base stock. The preparation method comprises the following steps of: 1, low-temperature autolysis: homogenizing fresh and alive Euphasia superb and treating through ultraviolet irradiation to realize autolysis; 2, protein extraction: adding water into homogenate, stirring and extracting, carrying out centrifugal separation to obtain an upper-layer liquid phase and precipitates; 3, protein stabilization: refrigerating the upper-layer liquid for storage or drying the upper-layer liquid before refrigerating the upper-layer liquid for storage; 4, protein preparation: thawing the refrigerated upper-layer liquid; adjusting pH value to 4.8-5.8 or heating for 10-30 minutes at 80-100 DEG C and then centrifuging to obtain precipitate products and carrying out spray drying to finally obtain the Euphasia superb protein base stock. The preparation method is simple to operate and is quite suitable for field operation on a fishing ship; through adoption of the preparation method, the protein recovery rate is up to 46%-51% and the fat recovery rate is up to 54%-60%; and in the prepared low-fluorine Euphasia superb protein base stock, the protein content is 70-78% / 100g of dry base stock, the fat content is 25-31g / 100g of dry base stock and the fluorine content is lower than 1.5 mcg. / g of dry base stock.

Description

technical field [0001] The invention relates to a method for preparing a protein base material using Antarctic krill. Background technique [0002] Antarctic krill (Euphausia superba) is one of the largest single species of biological resources on the earth. Its existing stock is estimated to be about 400-1.5 billion tons, and the annual output of mature shrimp is 300-500 million tons. About 100 million tons, forming a huge potential fishery resource. In recent years, with the gradual depletion of world-wide traditional fishery resources and the proposal of a 200 nautical mile exclusive economic zone, the huge Antarctic krill resources in Antarctic waters have attracted the attention of some pelagic fishery developed countries. my country has also included Antarctic krill resources as one of the main development species for the future development of pelagic fisheries. [0003] Antarctic krill contains 11.9% to 15.4% protein (moisture basis), which is one of the largest pro...

Claims

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Application Information

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IPC IPC(8): A23J1/04
Inventor 朱蓓薇杨静峰陈跃文吴海涛李冬梅李明
Owner DALIAN POLYTECHNIC UNIVERSITY
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