Highly-parallel micro flow channel chip applied to preparation of nanoparticles

Abstract

A microfluidic chip consisting of a PDMS top layer, a PDMS intermediate layer, a PDMS bottom layer, and a glass substrate. The PDMS top layer, the PDMS intermediate layer, and the PDMS bottom layer are all provided with a groove at the front-middle portion of their respective lower surfaces. When the PDMS top layer, the PDMS intermediate layer, the PDMS bottom layer, and the glass substrate are sequentially aligned and bonded, three layers of flow paths, namely a top layer flow path, an intermediate layer flow path, and a bottom layer flow path, are formed respectively by the groove of the PDMS top layer and the PDMS intermediate layer, by the groove of the PDMS intermediate layer and the PDMS bottom layer, and by the groove of the PDMS bottom layer and the glass substrate. Three fluid inlets respectively in communication with the top layer flow path, the intermediate layer flow path, and the bottom layer flow path are sequentially arranged on the upper surface of the PDMS top layer on the basis of the positional order of the three layers of flow paths, namely the top layer flow path, the intermediate layer flow path, and the bottom layer flow path, while 10 to 1000 fluid outlets are arranged at the end of each layer of flow path. The present invention employs a scheme of multiple paths in parallel and fluid envelopment at the fluid outlets to achieve uniformity control of a product, and to prevent the product from being clogged within the flow paths.

Description

technical field [0001] The invention relates to a high-parallel microfluidic chip used in the preparation of nanoparticles, which belongs to the direction of nano-pharmaceuticals. Background technique [0002] The existing literature has reported methods for synthesizing polymer nanoparticles with a size of tens to hundreds of nanometers. The main method is to use microfluidics to sandwich the organic phase of polymer molecules with hydrophilic and hydrophobic groups in water. Quite medium. [0003] The bulk mixing method in the prior art has the disadvantage of prone to inconsistent mixing and poor material uniformity. The polymer material PDMS ( The single flow channel prepared by polydimethylsiloxane) reduces the volume mixing from the scale of more than 100 microns to several microns. The characteristic diffusion reaction time without stirring can be shorter than the characteristic time of nanoparticle generation, and the reaction is more uniform. like figure 1 , f...

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Problems solved by technology

like figure 1 , figure 2 As shown, due to laminar flow, the organic phase is sandwiched by the water phase into a thickness of about several microns, and the organic solvent used is a liquid that is infinitely miscible with water. When the organic solvent diffuses into the water phase, the polymer soluble in the organic solvent Molecules will self-assemble into particles of tens of nanometers, with hydrophobic groups inside and hydrophilic groups outside. The average size is smaller than that of bulk mixing, and the uniformity is better than bulk mixing. The output can only reach a speed of about 20ul / h. At the same time, the surface of the polymer material PDMS is easy to adsorb molecules, causing blockage of the flow channel.

like image 3 , Figure 4 As shown, PLGA polylactic acid-glycolic acid copolymer-PEG polyethylene glycol: the improved 3D flow channel can avoid surface adsorption, but the output is still not improved, and more channels of fluid control are required to complete Single-channel output, it is difficult to obtain high-yield products, and the industrial application prospect is poor

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