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Method for preparing high-magnetization chitosan nanospheres

A technology of chitosan nanometer and chitosan solution, which is applied in the direction of nanotechnology, microsphere preparation, microcapsule preparation, etc., can solve the problems of wide distribution of magnetic particles, easy aggregation, and difficulty in control, and achieve a simple and repeatable preparation method. Good performance and high efficiency

Active Publication Date: 2012-07-18
ZIBO KANGBEI MEDICAL DEVICES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Magnetic nanoparticles such as ferroferric oxide are easy to aggregate due to the attractive force between particle dipoles, which limits its practical application value
The magnetic particles prepared by the embedding method have a wide distribution, irregular shape and difficult to control, the in-situ polymerization method is complicated, and the magnetic content of the obtained magnetic microspheres is low
Monomer polymerization is a common method for preparing magnetic polymer microspheres. Suspension polymerization is generally used to prepare micron-sized microspheres. Dispersion polymerization generally uses artificially synthesized polymers to prepare micron-sized to nano-sized microspheres, but artificially synthesized polymers Poor biocompatibility limits the biological application of magnetic nanospheres
Shen et al. (Colloids Surf B: Biointerfaces, 2009, 68: 1-6) reported a chitosan magnetic nanosphere loaded with pentafluorouracil prepared by emulsion polymerization, with a particle size of 100 ± 20nm, but this nano The specific saturation magnetization of the ball is only 3.4emu / g, and there is a problem of weak magnetic response in practical applications

Method used

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  • Method for preparing high-magnetization chitosan nanospheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Mix 1ml of 2% chitosan (Mw 5,000) acetic acid solution and 1ml of 10mg / ml nano ferric oxide particle aqueous solution, ultrasonic for 30 minutes, then slowly add the above solution dropwise to the solution containing 4% Span80 at a certain stirring rate In liquid paraffin, continue to stir for 15 minutes. The obtained primary emulsion was ultrasonicated in an ultrasonic cell breaker for 30 minutes with a power of 500 W. After 15 minutes of ultrasonication, 4 ml of glutaraldehyde solution was added dropwise to make the chitosan nanospheres carry out preliminary cross-linking. After the sonication, the preliminary cross-linked emulsion was transferred to a three-neck flask, and the cross-linking was continued for 12 hours under stirring at a temperature of 40°C. After the reaction is completed, it is centrifuged and washed with petroleum ether, isopropanol, ethanol and pure water to obtain the wrapped Fe 3 o 4 Magnetic chitosan nanoparticles with an average particle siz...

Embodiment 2

[0021] Mix 1ml of 2% chitosan (Mw 50,000) acetic acid solution and 1ml of 20mg / ml nano ferric oxide particle aqueous solution, ultrasonicate for 30 minutes, then slowly add the above solution dropwise to the solution containing 4% Span80 at a certain stirring rate In liquid paraffin, continue to stir for 15 minutes. The obtained primary emulsion was ultrasonicated in an ultrasonic cell breaker for 30 minutes, with a power of 400 W, and after 15 minutes of ultrasonication, 4 ml of glutaraldehyde solution was added dropwise to make the chitosan nanospheres carry out preliminary cross-linking. After the sonication, the preliminary cross-linked emulsion was transferred to a three-necked flask, and the cross-linking was continued for 12 hours under stirring at a temperature of 50°C. After the reaction is completed, the magnetic chitosan nanoparticles are obtained by centrifugal washing with petroleum ether, isopropanol, ethanol and pure water in sequence, and the average particle d...

Embodiment 3

[0023] Mix 1ml of 2% chitosan (Mw 200,000) acetic acid solution and 1ml of 15mg / ml nano ferric oxide particle aqueous solution, ultrasonicate for 30 minutes, then slowly add the above solution dropwise to the solution containing 4% Span80 at a certain stirring rate In the olive oil / petroleum ether (volume ratio 8 / 2), continue stirring for 15 minutes. The obtained primary emulsion was ultrasonicated in an ultrasonic cell breaker for 30 minutes with a power of 500 W. After 15 minutes of ultrasonication, 4 ml of glutaraldehyde solution was added dropwise to make the chitosan nanospheres carry out preliminary cross-linking. After the sonication, the preliminary cross-linked emulsion was transferred to a three-necked flask, and the cross-linking was continued for 24 hours under stirring at a temperature of 40°C. After the reaction is completed, the magnetic chitosan nanoparticles are obtained by centrifugal washing with petroleum ether, isopropanol, ethanol and pure water in sequen...

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Abstract

The invention provides a method for preparing high-magnetization chitosan nanospheres. according to the invention, an improved ultrasonic emulsification pre-crosslinking technique is adopted, namely, a chitosan solution in which superparamagnetic nanoparticles are dispersed is taken as a water phase, and liquid paraffin is taken as an oil phase, a high-magnetization and particle-size-controlled chitosan nanosphere is obtained through adjusting the composition of the oil and water phases and carrying out mixing, ultrasonic dispersion and ultrasonic pre-crosslinking,. The preparation method provided by the invention is simple, high in efficiency and low in cost; the highest saturation magnetization of the obtained chitosan nanosphere can be more than 45 emu / g; and the method has great application potentials in the fields of biological medicines, enzyme engineering and magnetic separation.

Description

technical field [0001] The invention belongs to the field of nano-biological materials and pharmaceutical preparations, in particular to a method for preparing chitosan nanospheres wrapped with superparamagnetic nanoparticles by means of ultrasonic emulsification and pre-crosslinking. Background technique [0002] Magnetic polymer microspheres have important application prospects in the fields of cell separation and purification, enzyme immobilization, drug delivery, and biological detection, and have received extensive attention in recent years (Adv Drug Deliv Rev, 2008, 60: 1252-1265; 26:3995-4021). Magnetic nanoparticles such as Fe3O4 are easy to aggregate due to the attractive force between particle dipoles, which limits its practical application value. In order to improve the biocompatibility and dispersion stability of superparamagnetic nanoparticles, they are usually modified with surfactants or polymer materials. According to different structures, magnetic polymer ...

Claims

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Application Information

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IPC IPC(8): B01J13/14B82Y40/00
Inventor 曲剑波靖广伦刘建国肖梓军朱虎
Owner ZIBO KANGBEI MEDICAL DEVICES
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