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Method for preparing scutellarin by using Aspergillus niger AS 3.795 for hydrolyzing scutellarin-7-O-glucuronide

A technology of scutellarin and scutellarin, applied in the field of scutellarin, can solve the problems of low conversion rate, environmental pollution, difficult control of reaction conditions and the like

Active Publication Date: 2012-08-01
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve technical problems such as difficult control of reaction conditions, low conversion rate and serious environmental pollution in the prior art, the present invention provides a method for preparing scutellarein with mild conditions, environmental protection and suitability for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 : Preparation of scutellarein by acid hydrolysis (Zhou Rongguang, Preparation, purification and structural characterization of scutellarein. Spectrum Laboratory, 2011, 28 (5): 2402-2406)

[0015] Weigh 15.0 g of scutellarin into a 1000 mL round bottom flask, add 100 mL of ethylene glycol, 100 mL of water and 10 mL of concentrated hydrochloric acid, heat and stir at 90°C for 5 h. TLC method was used to monitor the reaction process. After the reaction was completed, 500 mL of water was added to the reaction bottle, and a large amount of precipitation was precipitated. After standing at room temperature for 8 h, it was filtered, and the filtrate was washed with water until neutral, and dried in a vacuum oven at 60 °C for 48 h. 7.3 g of crude scutellarein was obtained.

[0016] Dissolve the crude scutellarein above in an appropriate amount of methanol, mix the sample with 20 g of silica gel (100-140 mesh), and evaporate the solvent in a fume hood. Then, it was ...

Embodiment 2

[0017] Example 2: Preparation of scutellarein by Aspergillus niger

[0018] a) Preparation of potato liquid medium: take 2kg of fresh potatoes, peel them, and cut them into 2cm 3 Small pieces, add 10L of water to boil, keep boiling for 30 minutes, filter the residue with gauze, add water to the filtrate to 10L, add 200g of glucose after constant volume. Distribute the prepared culture solution into 500mL Erlenmeyer flasks, each bottle contains 200mL culture solution, and can be divided into about 50 bottles. The above-mentioned 50 bottles of culture solution were sealed with gauze and kraft paper, and put into an autoclave in batches for sterilization. The sterilization condition is 115oC for 30min.

[0019] b) Culture of microorganisms: Aspergillus niger ( Aspergillus niger AS 3.795) was inoculated from the PDA solid medium into the above prepared potato liquid medium, placed in a shaker at 160rpm, and cultured at 26oC for two days.

[0020] c) Adding substrate: Disso...

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Abstract

The invention discloses a method for preparing scutellarin by using Aspergillus niger AS 3.795 for hydrolyzing scutellarin-7-O-glucuronide. Aspergillus niger AS 3.795 is used for hydrolyzing scutellarin-7-O-glucuronide glucuronyl to prepare the scutellarin. The method comprises the following steps of: using Aspergillus niger AS 3.795 to biologically transform a substrate scutellarin-7-O-glucuronide, using ethyl acetate to extract transformation products from fermentation liquor and using D101 macroporous adsorption resin column chromatography and recrystallization to purify the transformation products. The purity of the prepared transformation products can reach more than 99 percent according to high performance liquid chromatography. The method has the advantages that the defect that glucuronide is difficult to hydrolyze is overcome, the scutellarin can be prepared in a large scale under moderate conditions such as normal temperature and normal pressure, the environmental protection is facilitated, the production cost is reduced and the method is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the technical field of microbial transformation, extraction and separation of natural products, and in particular relates to converting scutellarein into scutellarein by using Aspergillus niger. Background technique [0002] Erigeron breviscapus, also known as Erigeron breviscapus, is a plant short scape flying canopy ( Erigeron breviscapus (Vant.) Hand-Mazz) is mainly distributed in Yunnan, Guangxi and other places. It tastes pungent, slightly bitter, and warm in nature. Modern pharmacological studies have shown that Erigeron breviscapus extract has the effects of reducing plasma viscosity, relaxing blood vessels, reducing vascular resistance, inhibiting platelet aggregation after ischemia, resisting ischemia-reperfusion injury, and improving brain function (Chinese herbal medicine, 2006, 37 (8 ): Attachment 9-Appendix 11), which has aroused people's widespread interest in the research on the active ingredients of Erigeron br...

Claims

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Application Information

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IPC IPC(8): C12P17/06C07D311/30C07D311/40C12R1/685
Inventor 邱峰陈丽霞杨立国康宁刘志辉
Owner SHENYANG PHARMA UNIVERSITY
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