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Supermolecular hydrogel and preparation method and application thereof

A technology of supramolecular hydrogel and weight-average molecular weight, which is applied in medical preparations of non-active ingredients, non-effective ingredients of polymer compounds, pharmaceutical formulations, etc., can solve the problems of little practical application value, low gene transfection efficiency, The material design is cumbersome and other problems, and the preparation method is convenient and fast, avoiding the influence of nucleic acid activity, and the concentration and temperature requirements are low.

Inactive Publication Date: 2013-06-26
广州新犇富科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the gene transfection efficiency of this gel material is very low, and its highest embodiment does not exceed 20%, so the practical application value is not large; and the design of the material is more loaded down with trivial details, and there are many synthesis steps

Method used

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  • Supermolecular hydrogel and preparation method and application thereof
  • Supermolecular hydrogel and preparation method and application thereof
  • Supermolecular hydrogel and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1. Drying of polyethylene glycol: The polyethylene glycol is vacuum dried at 80°C for 24 hours.

[0046] 2. Drying of dichloromethane and pyridine: add calcium hydride powder to dichloromethane or pyridine solution (add 1-2 grams of calcium hydride per 500ml solution), stir at room temperature for 24 hours and then distill.

[0047] 3. Synthesis of polyethylene glycol-p-toluate:

[0048] Dissolve 15 grams of dry polyethylene glycol (weight average molecular weight 5000) in 100 ml of a mixed solution of dichloromethane and pyridine. The volume ratio of dichloromethane to pyridine is 2:1; then add the mole number of polyethylene glycol. P-toluene sulfonyl chloride twice the alcohol, reacted for 48 hours at 30 degrees Celsius in the dark; after the reaction, the reaction solution was extracted with an equal volume of 3mol / L hydrogen chloride solution, and the organic layer was neutralized with 3 grams of sodium bicarbonate , Filter; then at 60 degrees Celsius to remove the methy...

Embodiment 2

[0056] 1. Drying of polyethylene glycol: The polyethylene glycol is vacuum dried at 80°C for 24 hours.

[0057] 2. Drying of dichloromethane and pyridine: add calcium hydride powder to dichloromethane or pyridine solution (add 1-2 grams of calcium hydride per 500ml solution), stir at room temperature for 24 hours and then distill.

[0058] 3. Synthesis of polyethylene glycol-p-toluenesulfonate:

[0059] Dissolve 15g of dry polyethylene glycol (weight average molecular weight 2000) in 100ml of a mixed solution of dichloromethane and pyridine. The volume ratio of dichloromethane to pyridine is 2:1; then add the moles of polyethylene glycol 2 Toluene sulfonyl chloride was reacted for 36 hours at 25°C in the dark; after the reaction, the reaction solution was extracted with an equal volume of 3mol / L hydrogen chloride solution, and the organic layer was neutralized and filtered with 4 grams of sodium bicarbonate ; Then at 50 ℃ conditions of rotary evaporation to remove the dichloromethan...

Embodiment 3

[0067] 1. Drying of polyethylene glycol: The polyethylene glycol is vacuum dried at 80°C for 24 hours.

[0068] 2. Drying of dichloromethane and pyridine: add calcium hydride powder to dichloromethane or pyridine solution (add 1-2 grams of calcium hydride per 500ml solution), stir at room temperature for 24 hours and then distill.

[0069] 3. Synthesis of polyethylene glycol-p-toluate:

[0070] Dissolve 15g of dry polyethylene glycol (weight average molecular weight 10000) in 100ml of a mixed solution of dichloromethane and pyridine, the volume ratio of dichloromethane to pyridine is 2:1; then add the mole number of polyethylene glycol 2 Toluene sulfonyl chloride was reacted for 48 hours at 25°C in the dark; after the reaction, the reaction solution was extracted with an equal volume of 3mol / L hydrogen chloride solution, and the organic layer was neutralized and filtered with 3g sodium bicarbonate; Then, the dichloromethane is removed by rotary evaporation at 60° C. to obtain polyet...

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Abstract

The invention discloses supermolecular hydrogel and preparation method and application thereof. The preparation method of the supermolecular hydrogel comprises the following steps of: synthesizing a polyethylene glycol modified cation copolymer; mixing with a nucleic acid solution and standing to obtain a copolymer / nucleic acid composite solution; and finally, mixing with alpha-cyclodextrin solution, and standing to obtain the supermolecular hydrogel capable of acting on a gene vector material. In the forming process of the hydrogel disclosed by the invention, a chemical crosslinking reactiondoes not happen, and thus the influence of a byproduct of the chemical crosslinking reaction on the activity of nucleic acid is effectively avoided, an efficient gene transfer effect is realized, andthe transfection efficiency is obviously improved over the conventional gel material; and moreover, the preparation method is convenient and quick, realizes formation at room temperature, imposes relatively low requirements on concentration and temperature, and is favorable for maintaining the biological activity of embedded gene and the biocompatibility of the material.

Description

Technical field [0001] The invention relates to the field of biomedical materials, in particular to a supramolecular hydrogel that can be used as a gene carrier, and a preparation method and application thereof. Background technique [0002] Gene therapy refers to the introduction of exogenous normal genes into target cells to correct or compensate for diseases caused by gene defects and abnormalities to achieve the purpose of treatment. It is currently an effective method for the treatment of cancer and congenital immune system diseases. The key to the implementation of this technology is to select a suitable gene vector and gene introduction method, so that the gene can be safely, efficiently, controlled and stable expression in the cell. As a type of biomedical material that can be used as a drug carrier, hydrogel has the characteristics of high loading rate, protection of macromolecular drugs from biological degradation or removal, and sustainable release of drug molecules. I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08G81/00C08G69/48C08G65/48C08G73/02C08L87/00C08L5/16A61K47/34A61K47/40C12N15/63
Inventor 武婕隆湘蕾
Owner 广州新犇富科技有限公司
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