Siderophilic endophyte with antagonism on banana fusarium wilt germs and application of siderophilic endophyte
A technology of banana fusarium wilt and banana, applied in the direction of application, chemicals for biological control, bacteria, etc., can solve the problems of high cost, manpower and funds, pollution of the environment, etc., and achieve good growth-promoting effect and good antagonistic effect Effect
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Embodiment 1
[0022] Embodiment 1, screening and identification of antagonistic bacteria of Fusarium wilt of banana
[0023] 1. Screening of antagonistic bacteria against Fusarium wilt of banana
[0024] This strain was isolated from healthy Brazilian banana (Musa AAA, CV. BAXI) plants in the Banana Germplasm Garden of the Institute of Tropical Crops Variety Resources, Chinese Academy of Tropical Agricultural Sciences. The specific method is: weigh healthy banana plants (planted for 8 months) 1g of tissue, after surface disinfection, put it into a sterilized mortar with 9mL of sterile water, add a little sterilized quartz sand to grind evenly, and let it stand for 15min. Take 0.1mL and dilute to 10 -3 、10 -4 、10 -5 Concentration gradient, take 0.1mL from each concentration gradient suspension, spread it on NA medium (beef extract 3g, bacteriological peptone 10g, NaCl 5g, agar 15g, distilled water 1000mL, pH 7.0-7.2) plate with a sterile applicator Above, use the sterile water that washe...
Embodiment 2
[0033] Embodiment 2, the determination of the endogenous property of Ralstonia sp. BEB99 CGMCC NO.6024
[0034] 1. Acquisition of rifampin resistance in Ralstonia sp. BEB99 CGMCC NO.6024
[0035] Inoculate Ralstonia sp. BEB99 into the NB culture solution containing 5 μg / mL rifampicin, shake the bacteria until the bacteria solution becomes cloudy, take a small amount of cultured bacteria solution and inoculate it into the NB culture solution containing 10 μg / mL rifampicin. In Fuping's NB culture medium, shake the bacteria until the bacteria become cloudy. According to this method, gradually increase the concentration of rifampicin for cultivation, and finally obtain the NB culture solution containing 400 μg / mL rifampicin (beef extract 3g, bacteriological peptone 10g, NaCl 5g, distilled water 1000mL, pH 7.0-7.2) Ralstonia sp. (Ralstonia sp.) BEB99 thallus of normal growth in the medium, confirmed the biological characteristic of this thalline and antibacterial activity and Rals...
Embodiment 3
[0038] Example 3, detection of siderophores produced by Ralstonia sp. BEB99 CGMCC NO.6024
[0039] Some microorganisms are able to produce a special kind of organic compound that can bind iron, called siderophore. Its main group is a derivative of hydroxamic acid or catechol, which can firmly bind to ferric iron, dissolve ferric iron and transport iron into cells.
[0040] Detect the siderophore ability of Ralstonia sp. BEB99 CGMCC NO.6024 of the present invention by following experiment:
[0041] 1. Preparation of siderophore medium plates (containers and utensils used in the configuration process must be soaked in 6mol / L HCl solution and rinsed with double distilled water):
[0042] 1) First prepare 10×MM9 medium (formula: 3g of potassium dihydrogen phosphate, 5g of sodium chloride, 10g of ammonium chloride, 1000mL of double distilled water), and autoclave at 121°C for 20min.
[0043] 2) Add 6mL of 10mmol / L hexadecyltrimethylammonium bromide (HDTMA) solution into a 100mL v...
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