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Reagent kit and method for extracting viral genome deoxyribonucleic acid (DNA) of human whole blood

A virus genome and kit technology, which is applied to viral genome DNA extraction kits and fields in human whole blood, can solve the problems of DNA loss, secondary pollution, and many steps, achieve large fragments, improve accuracy, and simplify operation steps Effect

Inactive Publication Date: 2012-09-19
缪林 +1
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Problems solved by technology

[0010] The purpose of the present invention is to provide a kit for rapidly and efficiently extracting viral genomic DNA from human whole blood and a method for extracting viral genomic DNA from human whole blood, in order to solve the problem caused by extracting viral DNA from serum. Defects such as secondary pollution, DNA loss and too many steps

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Embodiment Construction

[0038] The following examples are to further illustrate the present invention and should not be regarded as limiting the present invention.

[0039] Take the extraction of hepatitis B virus DNA in blood as an example to illustrate as follows:

[0040] Make the human whole blood virus DNA extraction kit according to the following formula:

[0041] 1. Red blood cell lysate: containing potassium bicarbonate 10mmol / L, ammonium chloride 150mmol / L, pH value 8.0 ethylenediaminetetraacetic acid (EDTA) 0.1mmol / L;

[0042] 2. White blood cell lysate: Tris-HCl (Tris-HCl) 20mmol / L with a pH value of 8.0, ethylenediaminetetraacetic acid (EDTA) 50mmol / L with a pH value of 8.0, dodecyl sulfate (SDS) )1.0%;

[0043] 3. Digestive fluid: containing 2.0% dodecyl sulfate (SDS);

[0044] 4. Purified solution: 6mol / L saturated sodium chloride solution;

[0045] 5. DNA storage solution: a sterilized Tris-HCl solution with a pH value of 8.5;

[0046] 6. Adsorption column: glass fiber membrane ad...

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Abstract

The invention relates to a reagent kit and a method for extracting viral genome deoxyribonucleic acid (DNA) of human whole blood. The reagent kit and a method for extracting the viral genome DNA of the human whole blood are characterized in that the reagent kit comprises red blood cell lysis buffer, white blood cell lysis buffer, digestive juice, purifying liquid and DNA preserving liquid, adopt two types of lysis buffer to respectively process a blood sample containing virus cells, and can efficiently and thoroughly lyse various virus cells while lysing blood cells, enable virus DNA to be released and enable the virus DNA to mutually wounded with blood genome DNA to be extracted. When the reagent kit is used for extracting the virus DNA, blood plasma and blood serum of blood are not required to be separated in advance, traces of high-purity viral DNA can be extracted only by taking fresh or frozen 0.1-1ml of anti-coagulating whole blood and can be completely unlinked and amplified efficiently. The reagent kit and a method for extracting the viral genome DNA of the human whole blood is suitable for scientific research units, laboratories for molecular genetic studies and medical treatment units, blood stations and disease control centers carrying out clinical genetic diagnosis technologies.

Description

technical field [0001] The invention relates to a kit for extracting viral genome DNA from human whole blood and a method for extracting viral genome DNA from human whole blood by using the kit. technical background [0002] Viruses are a type of non-cellular microorganisms that are small in size, simple in structure, and contain only a single nucleic acid. They must parasitize and multiply in living cells. Viruses are widely distributed in nature, can infect human cells, disintegrate in the cells and release nucleic acid molecules, and take advantage of the conditions of the intracellular environment to replicate with a unique life activity system, often causing human disease. [0003] Currently known DNA viruses include hepatitis B virus, T2 bacteriophage, smallpox virus, cauliflower mosaic virus, etc. The present invention is applicable to the extraction of viral genome DNA from the blood of all infected humans. Among known DNA viruses, hepatitis B virus It has the great...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12N15/33C12Q1/70C12Q1/68
Inventor 缪林张琼
Owner 缪林
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