Method for treating high-concentration wastewater of glutamic acid

A treatment method, glutamic acid technology, is applied in bipolar membrane electrodialysis to treat other waste liquids and high-concentration glutamic acid wastewater, which can solve the problems of high energy efficiency, reduce sewage treatment, reduce costs, and realize commercial utilization value effect

Active Publication Date: 2013-07-31
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of the invention not only solves the problem of separating effective components in high-concentration glutamic acid waste water, but also enables full utilization of glutamic acid-fermented bacterial protein and residual sugar, and has high energy efficiency, less waste water discharge, low pollution, and low electricity consumption. Dialysis technology has the advantages of simple operation, no pollution, and long service life. It not only reduces the investment cost of enterprises, but also saves energy and reduces emissions. It has multiple effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for treating high-concentration wastewater of glutamic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) The glutamic acid fermentation bacteria liquid uses a high-speed disk separator to separate the glutamic acid feed liquid and bacterial protein in the fermentation liquid. The speed of the high-speed disk separator to separate the bacteria is 4000-5000r / min

[0021] (2) Add the above bacterial protein into the stirring reactor, add appropriate amount of warm water and mix thoroughly, adjust the solid content to 8%, adjust the enzymolysis reaction temperature to 55°C, add a little sulfuric acid to adjust the pH to 6.5, add lysozyme 10kg / m3 respectively, acid Protease 25kg / m3, β-glucanase 5kg / m3, stir slowly, enzymolysis time is 6h, and use an amino acid analyzer to measure the contents of various amino acids in the enzymolysis solution. The above-mentioned enzymatic solution is separated and removed by a disc separator to remove the cell wall, and the obtained supernatant is evaporated at a low temperature by a three-effect plate evaporator. The material is directly ...

Embodiment 2

[0024] Using the enzymolyzed protein paste obtained in Example 1 as a nitrogen source to replace commercially available yeast powder, a threonine fermentation test was carried out, and the average fermentation parameters of 5 consecutive tanks were respectively:

[0025] Using the enzymolyzed protein paste prepared in Example 1 as a nitrogen source, the fermentation broth produced 13% acid, the fermentation period was 28 hours, and the fermentation sugar-acid conversion rate was 68%.

[0026] Using commercially available yeast powder as a nitrogen source, the fermentation broth produced 14% acid, the fermentation period was 28 hours, and the fermentation sugar-acid conversion rate was 67%.

[0027] Through the above comparison, the product produced by the present invention can completely replace commercially available yeast powder products for threonine fermentation, thereby greatly reducing the cost of threonine fermentation.

[0028] Through high-performance liquid chromatog...

Embodiment 3

[0030] The enzymolyzed protein paste prepared in Example 1 is dried by spray granulation fluidized bed, and the obtained finished product is packaged into enzymatic bacterial protein powder (hot air temperature of spray granulation fluidized bed<150°C), replacing the standard The yeast powder in the YPD medium, and the rest of the ingredients remain unchanged. Saccharomyces cerevisiae and Pichia pastoris are cultivated under the same conditions, and the culture effect of the product is evaluated by comparing the growth of the cells.

[0031]

[0032] Determination of OD by nephelometric method 600 The growth of the cells was characterized, indicating that this product can replace commercially available yeast powder products and achieve the desired effect.

[0033] Taking Hulunbeier Northeast Fufeng Biotechnology Co., Ltd. annual output of 200,000 tons of glutamic acid production workshop as an example, the cost analysis compared with the traditional process after adopting t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for treating high-concentration wastewater of glutamic acid, in particular a method for fermenting discarded thalli by utilizing the enzymolysis of a compound enzyme and treating other waste liquid by utilizing the electroosmosis of a bipolar membrane. By the method, the problem that active ingredients cannot be separated from the high-concentration wastewater of the glutamic acid is solved, glutamic acid fermentation residual sugar can be utilized fully, energy efficiency is high, the discharge of the wastewater is a few, and the pollution is low; and an electroosmosis technology has the advantages of simplicity of operation, no pollution, long service life and the like, the investment cost of enterprises is reduced, and the effects of energy conservation,emission reduction and multiple purposes are achieved.

Description

technical field [0001] The invention belongs to the technical field of glutamic acid production, and relates to a method for treating high-concentration glutamic acid waste water, in particular to a method for using compound enzymes to enzymolyze and ferment waste cells and using bipolar membrane electrodialysis to treat other waste liquids. Background technique [0002] With the rapid development of the economy and the continuous improvement of technology, my country has become a major producer and consumer of monosodium glutamate, but the amount of wastewater discharged during the production of monosodium glutamate is large, and the mother liquor discharged from the monosodium glutamate fermentation broth after isoelectric extraction of glutamic acid has CODCr High, high BOD5, high bacteria content, high sulfate radical (chloride ion before pH adjustment with sulfuric acid) content, high ammonia nitrogen content and low pH value (1.5-3.2) are characterized by "five highs and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C02F9/10C02F1/04C02F1/38C02F1/469C02F1/44C02F1/42C02F103/36
Inventor 郇月伟唐永强冯珍泉胡俊礼郭永波张红静
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap