Staphylococcus sp. LZ16 strain and application thereof

A staphylococcus, LZ16 technology, applied in the direction of application, bacteria, fungicides, etc., can solve the problems that have not been reported, and achieve the effect of improving production performance and promoting the prevention and control of rice blast

Inactive Publication Date: 2013-06-19
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, more than 90% of the research and development of biopesticides are insecticides. Fungicides are mainly aimed at wheat stripe rust, rice stripe leaf blight, etc. The creation of biogenic pesticides for the control of rice blast has not been reported so far.

Method used

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  • Staphylococcus sp. LZ16 strain and application thereof
  • Staphylococcus sp. LZ16 strain and application thereof
  • Staphylococcus sp. LZ16 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The cultivation and identification of embodiment 1 Staphylococcus LZ16 strain bacterial strain

[0040] (1) Isolation and cultivation of marine bacteria

[0041] Sample source: Nan'ao seawater and sea mud mixture (taken from Nan'ao Linhai Experimental Station of Shantou University, Wuping Village, Shen'ao Town, Nan'ao County, Shantou City, Guangdong Province). Take the sample (seawater, sea mud mixture) (seawater 20ml, sea mud 5g), put it into a sterilized Erlenmeyer flask, add 30ml sterilized artificial seawater (NaCl 23.4g, MgSO 4 12g, KCl 1.5g, CaCl2 2.2g, distilled water 1000ml), shake vigorously (200r / min) to make a uniform suspension, let it stand, filter, take the filtrate, take 0.1ml sterile artificial seawater gradient dilution 10 -1 -10 -7 Gradient, draw 0.1ml of the diluted solution and spread it on the screening medium (Zobell 2216E: tryptone 5g, yeast extract 1g, high ferric phosphate 0.1g, agar powder 20g, artificial seawater 1000ml, pH7.6) plate, 37 ℃ ...

Embodiment 2

[0085] Embodiment 2 staphylococcus LZ16 resistance to blast fungus test

[0086] (1) Tablet confrontation experiment

[0087] Cultivate LZ16 bacteria for 12 and 24 hours respectively, and then ultrasonically crush to obtain crude extracts: unlysed supernatant, lysed supernatant, and lysed precipitate. Each 10ml of three crude extracts is divided into two parts, each 5ml, one part is heated and the other is not heated. Then, when the PDA medium was cooled to about 50°C, 5ml of different crude extracts were added, mixed evenly and poured onto the plate. After the plate is cooled, inoculate the mycelia block of Magnaporthe oryzae in the center (with a diameter of 5mm, use a 1ml pipette tip used with a standard pipette to punch holes upside down), culture in a constant temperature box at 28°C for 4 days, and measure Magnaporthe oryzae plaque diameter ( figure 2 ). Such as figure 2 As shown, the diameter of yc-25 plaque was the smallest under the lysed supernatant of LZ16 bac...

Embodiment 3

[0092] Antibacterial active substance stability test of embodiment 3 Staphylococcus LZ16 strain

[0093] (1) Thermal stability experiment

[0094] The lysed supernatant of the 24-h fermentation broth of Staphylococcus LZ16 strain was treated at -20°C, 7°C, 28°C, 37°C, 45°C, 55°C, 65°C, and 80°C for 30 minutes, respectively. Take 300 μl each and mix with 200 μl Magnaporthe oryzae spore suspension (spore concentration is about 2.5×10 5 cells / ml) were mixed evenly and placed on a glass slide, cultured in a 28°C incubator for 7 hours, and observed under a microscope to compare the germ tube length ( Figure 5 ).

[0095] (2) pH stability test

[0096] The lysed supernatant of the 24h fermentation broth of Staphylococcus LZ16 strain was placed at 4°C for 24h under the conditions of pH 2, 4, 6, 7, 8, and 10, respectively. Take 300 μl each and mix with 200 μl Magnaporthe oryzae spore suspension (spore concentration is about 2.5×10 5 cells / ml) were mixed evenly and placed on a gl...

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Abstract

The invention provides a staphylococcus sp. LZ16 strain, which has a collection number of CGMCC (China General Microbiological Culture Collection Center) No.6121. The staphylococcus sp. contains Purine nucleoside phosphorylase (PNP) protein and has an inhibiting effect on plant pathogenic bacteria. The amino acid sequence of the staphylococcus sp. is disclosed in SEQ ID (Identity) No.3, and the nucleotide sequence of a gene for encoding the antibacterial protein is disclosed in SEQ ID No.2. The staphylococcus sp. LZ16 strain provided by the invention has an obvious inhibiting effect on the pyricularia oryzae. Antibacterial agent or biopesticide prepared from the strain LZ16 disclosed by the invention can generate obvious acceleration action on preventing and curing the pyricularia oryzae,the staphylococcus sp. LZ16 strain can be popularized and used in agricultural production, and the production performance of paddy is effectively improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Staphylococcus LZ16 strain with antagonistic effect on rice blast and application thereof. Background technique [0002] Rice blast, bacterial blight and sheath blight are listed as the three major diseases of rice. Rice blast is a fungal disease of rice caused by Magnaporthe grisea. It has a wide distribution range. Since the 1990s, the annual occurrence area of ​​rice blast in my country has been more than 57 million mu, and the annual loss of rice has reached hundreds of millions of kilograms. The annual occurrence area of ​​rice blast in Guangdong is not less than 500,000 mu, and there is an increasing trend year by year. The occurrence of rice blast not only affects the yield, but also affects the quality of rice. Therefore, the research on rice blast fungus has received widespread attention from rice scientific research and rice production, and has achieved gratifying achiev...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/12C12N15/54C12N15/63A01N63/00A01N63/02A01P3/00A01P21/00C12N15/11C12R1/44
Inventor 刘柱余琴孙群张维朱建清林敏
Owner SHANTOU UNIV
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