Bacillus methylotrophicus UTM401 and applications thereof
A technology of bacillus and methyl nutrition, applied in the direction of application, bacteria, fungicides, etc., to shorten the fermentation cycle, promote plant growth, and increase crop yield
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0032] Example 1 Isolation and Identification of Methylotrophic Bacillus UTM401
[0033] Weigh 10g of sludge decomposed compost sample (collected from the compost fermentation workshop of Dadi Lvyuan Environmental Protection Technology (Beijing) Co., Ltd.) and place it in a 100ml sterile water 250ml triangular flask with 10 small glass beads, and shake it at a constant temperature at 40°C. Shake on the bed for 1 hour, then let it stand for 30 minutes, in the ultra-clean bench, absorb 1ml of the supernatant, and transfer it to 100ml of sterilized acclimation medium (3g of beef extract, 3g of yeast extract, 8g of peptone, chloride 3 g of sodium, 10 g of soluble starch, and 1000 ml of distilled water), the enrichment and acclimatization culture was shaken on a constant temperature shaker at 40 ° C for 3 days, and the rotation speed was 160 r / min. In the same way, 1ml of the enrichment solution was drawn again, and subcultured for another 3 generations under the same conditions. ...
Embodiment 2
[0037] The preparation of embodiment 2 strain UTM401 fermented seed liquid (1)
[0038] Transfer the slant surface of the production strain UTM401 of the present invention stored at 4°C to the NA medium plate, cultivate it at 30°C until it is thick, and then use a sterile glass spatula to transfer all of it to a 5000ml triangle containing 1000ml LB liquid medium In the bottle, culture on a shaker at 37°C with a rotation speed of 160r / min for 2 days. At this time, the OD 600 2.202, stop the cultivation, this is the primary fermentation seed liquid.
[0039] The primary fermentation seed liquid was inoculated into a fermentation seed tank containing LB liquid medium at 5% of the volume ratio of the liquid medium for fermentation. The fermentation conditions are temperature 37°C, intermittent stirring and ventilation, stirring once every 1 hour, stirring for 5 minutes each time, stirring speed 150r / min, ventilation once every half an hour, 5 minutes each time, ventilation rate 1...
Embodiment 3
[0041] The preparation of embodiment 3 strain UTM401 fermented seed liquid (2)
[0042] Transfer the slant surface of the production strain UTM401 of the present invention stored at 4°C to the NA medium plate, cultivate it at 30°C until it is thick, and then use a sterile glass scraper to transfer all of it to a 5000ml triangle containing 2000ml LB liquid medium In the bottle, culture on a shaker at 30°C with a rotation speed of 150r / min for 3 days. At this time, the OD 600 It is 2.084, stop cultivating, this is the primary fermentation seed liquid.
[0043] The primary fermentation seed liquid was inoculated into the fermentation seed tank containing LB liquid medium according to 10% of the volume ratio of the liquid medium to carry out fermentation culture. Fermentation conditions are temperature 30°C, intermittent stirring and ventilation, stirring once every 1 hour, stirring for 10 minutes each time, stirring speed 120r / min, ventilation once every half an hour, 5 minutes ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More