Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Trichoderma harzianum strain and application in prevention and control of phytophthora capsici Leonian thereof

The technology of pepper blight and pepper blight is applied to Trichoderma harzianum strains and its application field in the prevention and control of pepper blight, and can solve the problems of fungicide resistance or even drug resistance, easy environmental pollution, and high cost of chemical control, Achieve the effect of increasing farmers' income, broad application prospects and enhancing economic added value

Active Publication Date: 2012-10-10
中农绿康(北京)生物技术有限公司
View PDF2 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical control costs are high and the environment is easy to pollute, and pathogens are prone to resistance or even resistance to fungicides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Trichoderma harzianum strain and application in prevention and control of phytophthora capsici Leonian thereof
  • Trichoderma harzianum strain and application in prevention and control of phytophthora capsici Leonian thereof
  • Trichoderma harzianum strain and application in prevention and control of phytophthora capsici Leonian thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1. Obtainment and identification of Trichoderma harzianum HNA-12

[0055] 1. Obtaining strain HNA-12

[0056] 1. Sample collection

[0057] The samples were collected from the surface rhizosphere soil of pepper crops in Anyang, Henan.

[0058] Use soil borrowing equipment to remove 3-5cm of the topsoil in the field, dig out the soil together with the roots of the plants, pack them in polyethylene plastic bags, and bring them back to the laboratory for separation.

[0059] 2. Obtaining strain HNA-12

[0060] Air-dry the roots attached to the soil a little, tap the roots gently to make the attached soil fall off, dilute them gradually with sterile water, pipette 0.1 mL of the dilutions into the TSM medium plate, and use a sterile L-shaped coating The cloth rod is evenly coated and placed in a constant temperature incubator at 25-28°C for 3-4 days. Pick a single colony resembling Trichoderma and transfer it to a PDA plate for purification and culture. The mirror will initia...

Embodiment 2

[0067] Example 2. Inhibition of Trichoderma harzianum HNA-12 on pathogens

[0068] Experimental group: Inoculate Trichoderma harzianum HNA-12 and pathogenic bacteria cakes on the same PDA plate for confrontation culture (the vertical distance between the centers of the two bacterial cakes is 3 cm, and the diameter of the two bacterial cakes is 5 mm); The PDA plate of the pathogenic bacteria cake was used as the control group; after a certain period of dark culture at 25°C, the colony diameter of the pathogen (the average of the longest diameter and the shortest diameter) was measured and the inhibition rate was calculated. Inhibition rate = (colony diameter of pathogenic bacteria in the control group-colony diameter of pathogenic bacteria in the experimental group) / colony diameter of pathogenic bacteria in the control group * 100%. Three repeated treatments were set for each pathogenic bacteria, and three repeated treatments were set for the control, and the results were averag...

Embodiment 3

[0073] Example 3 Greenhouse biocontrol effect experiment of Trichoderma harzianum HNA-12

[0074] The pepper seeds are soaked in a warm soup at 55°C for 30 minutes, and then germinated. Select the same germinated seeds and sown in a culture bowl, 20 seeds per bowl; cultivate in a greenhouse (25-30°C) until the pepper plants grow 4 true leaves, grouping is as follows Operation (3 culture bowls per group):

[0075] Experimental group: each pepper plant was inoculated with 10 mL of Trichoderma harzianum HNA-12 spore suspension (the spore concentration was 5×10 6 Pieces / mL) and 10mL of Capsicum Phytophthora spore suspension (spore concentration is 5×10 6 Pcs / mL); The method of inoculation is to inject the spore suspension into the rhizosphere soil with a syringe;

[0076] Control group: Inoculate 10 mL of spore suspension of Phytophthora capsici per plant (spore concentration is 5×10 6 Pcs / mL); the method of inoculation is to inject the spore suspension into the rhizosphere soil with a s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a trichoderma harzianum strain and application in prevention and control of phytophthora capsici Leonian thereof. The trichoderma harzianum (HAN-12) provided by the invention, the preservation number of which is CGMCC No. 5989, can be used for restraining pathogenic bacteria, promoting the growth of a hot pepper plant, and preventing and controlling the phytophthora capsici Leonian of the hot pepper plant. The trichoderma harzianum strain can have a wide application prospect in the sustainable agricultural development.

Description

Technical field [0001] The invention relates to a Trichoderma harzianum strain and its application in preventing and controlling pepper blight. Background technique [0002] Pepper blight is a devastating fungal disease that occurs on peppers. The disease can cause the disease of many crops in different regions and cause a lot of economic losses. Typical symptoms of pepper blight: a few days after the plants are infected, the rhizome tissue rots until a large number of plants die. So far, there are no varieties with good resistance to pepper blight in production. [0003] At present, chemical control occupies an important position in the control of pepper blight at home and abroad. Chemical control costs are relatively high and easily pollute the environment, and pathogens are likely to be resistant to fungicides or even drug resistance. Therefore, biological control has gradually become the focus and focus of people's attention. Summary of the invention [0004] The purpose of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P21/00C12R1/885
Inventor 王琦姚彦坡李燕
Owner 中农绿康(北京)生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com