Method for degrading nitrobenzene in waste water

A technology for nitrobenzene and waste water, applied in the field of nitrobenzene, can solve problems such as poor metabolism of nitrobenzene, and achieve the effects of low cost, guaranteed degradation rate, and good degradation

Inactive Publication Date: 2012-10-17
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of the poor metabolism of bacteria to nitrobenzene in a low temperature environment, so that nitrobenzene can be degraded better in a low temperature environment. Based on this purpose, the present invention provides a kind of A method for efficiently degrading nitrobenzene

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  • Method for degrading nitrobenzene in waste water
  • Method for degrading nitrobenzene in waste water
  • Method for degrading nitrobenzene in waste water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Inoculate the bacterial suspension of strain cc-2 in the logarithmic growth phase at 10% (v / v) in the nitrobenzene inorganic salt medium (inorganic The main formula of the salt medium is: 3.8g of dipotassium hydrogen phosphate, 1g of potassium dihydrogen phosphate, 1g of sodium chloride, 0.1g of ammonium chloride, 0.2g of magnesium sulfate, 2g of glucose, made into 1000ml with distilled water), at 15℃ , pH = 7, 140r / min conditions shaking culture for 48 hours. Utilize gas chromatography to measure the nitrobenzene degradation rate of bacterial strain 48h, degradation result is as follows figure 2 .

[0024] Such as figure 2 As shown, the degradation rate of strain p-nitrobenzene decreases with the increase of the initial concentration of nitrobenzene, and the degradation rate is 80.64% at 50 mg / L, and drops to 60.94% at 200 mg / L

Embodiment 2

[0026] OD 600 The strain cc-2 bacteria suspension of =2 is inoculated to the nitrobenzene inorganic salt medium (inorganic salt medium) with a nitrobenzene concentration of 200mg / L by 1%, 5%, 10%, 15% and 20% volume fraction The main formula is: 3.8g of dipotassium hydrogen phosphate, 1g of potassium dihydrogen phosphate, 1g of sodium chloride, 0.1g of ammonium chloride, 0.2g of magnesium sulfate, 2g of glucose, made into 1000ml with distilled water), at 15℃, pH = 7. Culture with shaking at 140r / min for 48 hours. Utilize gas chromatography to measure the nitrobenzene degradation rate of bacterial strain 48h, utilize photoelectric turbidimetry to measure the growth situation of bacterial strain simultaneously, degradation result is as follows image 3 .

[0027] Such as image 3 As shown, with the increase of the inoculum size, the strain OD 600 increased, the growth of the strain was good, and the degradation rate of nitrobenzene also increased, but when the inoculation am...

Embodiment 3

[0029] Inoculate the bacterial suspension of strain cc-2 in the logarithmic growth phase at 10% (v) to nitrobenzene with a pH value of 4, 5, 6, 7, 8 and 9 and a nitrobenzene concentration of 200mg / L Inorganic salt medium (the main formula of inorganic salt medium is: 3.8g of dipotassium hydrogen phosphate, 1g of potassium dihydrogen phosphate, 1g of sodium chloride, 0.1g of ammonium chloride, 0.2g of magnesium sulfate, 2g of glucose, made into 1000ml with distilled water ), cultured with shaking at 15°C and 140r / min for 48h. Utilize gas chromatography to measure the nitrobenzene degradation rate of bacterial strain 48h, utilize photoelectric turbidimetry to measure the growth situation of bacterial strain simultaneously, degradation result is as follows Figure 4 .

[0030] Such as Figure 4 As shown, when the pH value increased from 4 to 7, the strain OD 600 and degradation rate were significantly increased, and when the pH was greater than 7, the strain was inhibited, and...

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Abstract

The invention relates to a method for degrading nitrobenzene in waste water, characterized by inoculating nitrobenzene low temperature-resistant bacteria in logarithmic growth phase to the waste water containing nitrobenzene according to a volume percent of 1-20%, regulating the pH value to 4-9, and culturing for 48 h under the conditions of vibration, wherein the nitrobenzene low temperature-resistant bacteria are acinetobacter baumannii, preferably acinetobacter baumannii strain cc-2. According to the invention, nitrobenzene can be effectively degraded in a low temperature environment, the defects of restraint of growth and metabolism of the strain in low temperature and insufficient capability of degrading nitrobenzene are made up, the capacity of the strain for degrading nitrobenzene in low temperature is raised, and the method provides the treatment of nitrobenzene in low temperature with a technology support.

Description

technical field [0001] The invention belongs to the field of environmental protection, and in particular relates to a method for degrading nitrobenzene in waste water by using Acinetobacter baumannii strain cc-2. Background technique [0002] Nitrobenzene is a colorless or light yellow highly toxic substance with a bitter almond smell. It is insoluble in water, easily soluble in ethanol, ether, benzene and oil, and difficult to biodegrade. It has been listed as the world's "environmental priority control Toxic Organic Pollutants". It is an important chemical raw material widely used in the production of medicines, pesticides, synthetic dyes, dyes, plastics and explosives. The rapid development of the global chemical industry has increased the demand for nitrobenzene, and the related pollutants entering the environment have also increased. The large amount of wastewater containing nitrobenzene has caused a serious threat to environmental safety. my country requires the mass...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/38
Inventor 徐成斌马溪平孟雪莲邵亮李银萍李瑶瑶姜磊
Owner LIAONING UNIVERSITY
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