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Method for preparing and extracting carotenoid from microbial thalli

A technology of microbial bacteria and carotene, applied in the field of bioengineering and bioseparation engineering, can solve the problems of high energy consumption and other costs, large amount of organic solvents, industrial application limitations, etc., to reduce energy consumption and other costs, and achieve acceptable quality. The effect of control and dosage reduction

Active Publication Date: 2014-03-12
HUAZHONG UNIV OF SCI & TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These extraction methods all require dehydration and drying and bacterial crushing processes, the process is long, the single extraction yield is low and takes a long time, the amount of organic solvent is large, the energy consumption and other costs are high, and the industrial application is limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] After the fermentation of B. trispora was finished, the mixture was left to settle, and the supernatant was discarded to obtain wet thallus. The wet bacteria are introduced into the explosion tank through the logistics pipeline, heated with 0.3MPa steam to a saturated steam pressure of 0.1MPa steam explosion, centrifuged to obtain the filter cake of the bacteria, take 200g of the filter cake of the bacteria, and use 5L of acetone to stir and leach for 30 minutes at 50°C , filtered to obtain a clarified extract, vacuum concentrated at 50°C and a vacuum degree of 0.01MPa, recovered acetone, and obtained carotenoid oil containing β-carotene, γ-carotene and lycopene. The recovery rate of β-carotene reaches 90%, the recovery rate of γ-carotene reaches 93%, and the recovery rate of lycopene reaches 92%.

Embodiment 2

[0022] After the fermentation of B. trispora is finished, wet mycelium is obtained by centrifugation. The wet bacteria are introduced into the blasting tank through the logistics pipeline, heated to a saturated vapor pressure of 0.2 MPa with 0.1MPa steam, steam explosion, vacuum filtration to obtain the filter cake of the bacteria, take 2Kg of the wet filter cake of the bacteria, and use 50L ethanol acetone mixed solvent (ethanol The volume percentage content is 30%), stirred and extracted at 35°C for 50 minutes, filtered to obtain a clear extract, concentrated in a vacuum at 40°C and a vacuum of 0.05MPa, recovered the organic solvent, and obtained β-carotene, γ-carotene Carotenoid oils with lycopene and lycopene. The recovery rate of β-carotene reaches 93%, the recovery rate of γ-carotene reaches 92%, and the recovery rate of lycopene reaches 95%.

Embodiment 3

[0024] After the fermentation of B. trispora was finished, the mixture was left to settle, and the supernatant was discarded to obtain wet thallus. The wet bacteria are introduced into the explosion tank through the logistics pipeline, heated to a saturated vapor pressure of 0.15MPa with 0.6MPa steam, and the filter cake of the bacteria is obtained by plate and frame filtration. Take 20Kg of the wet bacteria filter cake, and use 300L ethanol acetone mixed solvent (ethanol The volume percentage content is 50%), stirred and extracted at 40°C for 45 minutes, filtered to obtain a clear extract, concentrated in vacuo at 60°C and a vacuum of 0.08MPa, recovered ethanol and acetone, and obtained β-carotene, γ-carotene Carotenoid oils with lycopene and lycopene. The recovery rate of β-carotene reaches 93%, the recovery rate of γ-carotene reaches 93%, and the recovery rate of lycopene reaches 94%.

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Abstract

The invention which belongs to bioengineering and bioseparation engineering fields relates to a method for preparing and extracting carotenoid from microbial thalli. The method comprises the following steps: fermenting the microbial thalli, separating the resulting fermentation solution and the thalli to obtain wet thalli, guiding the wet thalli to an explosion tank through a material flow pipeline, and carrying out high-pressure explosion wall breaking; dehydrating the wet thalli with disrupted cells to obtain a filter cake formed by thallium fragments; adding 10-25L of an organic solvent to each 1kg of the filter cake, and carrying out stirring extraction at 30-55DEG C for 20-50min; filtering to obtain an organic solvent extraction containing carotenoid grease; and carrying out vacuum concentration on the extraction at 40-60DEG C, and recovering the organic solvent to obtain the carotenoid grease. The extraction yield one time reaches above 90%. The method has the advantages of omission of dehydration drying and thallium crushing operations in traditional technologies, short process flow, high extraction yield and short time each time, reduced organic solvent application amount, substantially reduced energy consumption and other costs, simple whole process, and controllable quality, and is suitable for industrialized production applications.

Description

technical field [0001] The invention belongs to the fields of bioengineering and bioseparation engineering, and in particular relates to a method for extracting and preparing carotenoids from microbial cells. The method is especially suitable for mass extraction and preparation of carotenoids from Blakeslea trispora and Phaffia rhodozyme. Background technique [0002] β-carotene, γ-carotene, lycopene, and astaxanthin are the four main carotenoids in microorganisms. β-carotene, lycopene, and astaxanthin are lipophilic compounds with very high market value. It is a natural pigment in the food industry, an anti-aging antioxidant in the cosmetic industry, and an anti-cancer drug in the pharmaceutical industry. The demand for these carotenoids in the food, cosmetics, and pharmaceutical industries is also increasing, and the market prospect is good. At present, lycopene is mainly extracted from tomato, and β-carotene is mainly extracted from salina. The insufficient supply of r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00C07C403/24C07C403/02C07C11/21C07C7/00
Inventor 余龙江王红波鲁明波何谧何峰
Owner HUAZHONG UNIV OF SCI & TECH
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