cDNA sequence and amino acid sequence of lugworm fibrinolytic enzyme with thrombolysis activity

A sea earthworm and fibrinolytic enzyme technology, applied in the field of medical bioengineering
CN102757973AInactive Publication Date: 2012-10-31WEIFANG MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WEIFANG MEDICAL UNIV
Publication Date
2012-10-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a cDNA sequence and an amino acid sequence of coding lugworm fibrinolytic enzyme which are respectively shown in SEQIDNo.1 and SEQIDNo.2 in a sequence table, and belongs to the field of medical biological engineering. The cDNA sequence has the beneficial effects that according to the cDNA sequence of the lugworm fibrinolytic enzyme, the coding gene of the fibrinolytic enzyme can be cloned from digestive tract tissue of lugworm, and can be expressed in a prokaryotic expression system or a eukaryotic expression system through a gene recombination technology. The recombinant lugworm fibrinolytic enzyme has plasminogen activation activity, and can be used as a new thrombolytic medicament in genetic engineering.
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Description

[0001] technical field

[0002] The invention relates to a sea earthworm fibrinolytic enzyme, specifically a cDNA sequence and an amino acid sequence of the sea earthworm fibrinolytic enzyme with thrombolytic activity, belonging to the field of medical bioengineering. Background technique

[0003] Thrombotic disease is a serious threat to human health, and it is urgent to find a new type of thrombolytic agent that is safe, efficient and low-toxic. Fibrinolytic enzymes mostly belong to the serine protease family. The protease catalytic active center of this family contains a highly conserved sequence GDSGGP near Ser, which mainly cleaves the peptide bond at the carboxyl end of Lys or Arg in fibrin and fibrinogen to degrade it into Many soluble small peptides have anticoagulant effects; some plasmin also acts as a plasminogen activator and indirectly exerts a thrombolytic effect. The clinically approved fibrinolytic enzymes include urokinase, streptokinase, staphylokinase, an...

Claims

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