Method for detoxifying and reproducing helianthus tuberosus quickly

A technology of Jerusalem artichoke and test-tube seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low seed setting rate, low germination rate, increased planting cost, and consumption of large tubers, etc., and achieves short induction of adventitious buds , reduce planting costs, and shorten the growth time

Active Publication Date: 2012-11-07
北京碧青园生态环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the extremely low seed setting rate and seed germination rate of Jerusalem artichoke, the conventional propagation method in production is tuber propagation, but this asexual reproduction method will cause the accumulation of viruses with the increase of the number of culture generations, resulting in the deterioration of varieties and the reduction of yield , in addition, when carrying out large-scale planting, need to consume a large amount of seed tubers, increased planting cost

Method used

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  • Method for detoxifying and reproducing helianthus tuberosus quickly
  • Method for detoxifying and reproducing helianthus tuberosus quickly
  • Method for detoxifying and reproducing helianthus tuberosus quickly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Store the harvested Jerusalem artichoke tubers at 4°C for 2 months, cut off the bud points and place them in a dark environment at 25-28°C for sand culture. After the sprouts start to germinate, transfer to light culture. 7-10cm, cut off and remove the leaves, rinse with tap water for one hour, and then carry out disinfection treatment: soak in 70% vt alcohol for 30s, rinse with sterile water three times, soak in 4%wt sodium hypochlorite for 5-7min, soak in sterile water Rinse 5 times. Test-tube seedling culture: cut out the stem tip and the stem segment with axillary buds, and insert them into the bud induction medium. When the adventitious buds grow to 2-3cm, they can be cut and transferred to the rooting medium for rooting induction, and finally the obtained complete test tube After hardening, the seedlings are transplanted to the field for cultivation and management.

[0022] The above-mentioned bud induction medium is obtained by adding Jerusalem artichoke tubers ...

Embodiment 2

[0028] Cut off the adventitious buds induced on the bud induction medium in Example 1 when they grow to 5-6cm, remove the leaves, cut off the stem segment or shoot tip with axillary buds as explants, and then transfer to the bud induction medium to carry out Value-added culture, after 5-10 generations of continuous value-added, rooting, seedling hardening, and finally transplanting to the field, these seedlings are still strong and maintain good vitality, and their basic characteristics are still consistent with the original mother plant. The specific implementation effect is shown in Table 2.

[0029] Table 2 Comparison of growth parameters between virus-free seedlings and non-virus-free seedlings

[0030]

Embodiment 3

[0032] A method for detoxification and rapid propagation of Jerusalem artichoke. The axillary buds or stem tips of Jerusalem artichoke are used as explants. After disinfection, they are transferred to a bud induction medium for test-tube seedling cultivation. When the buds grow to 2-3cm, the buds are transferred to Rooting medium, when the root grows to 3-4cm, the complete test-tube plantlet is hardened and transplanted into the field. The bud induction medium is based on MS, containing 30g L -1 Sucrose, 7g·L -1 Agar, 67g·L -1 Jerusalem artichoke tubers, 0.1mg·L -1 NAA and 0.5mg·L -1 6-BA, pH 5.8. The rooting medium is based on 1 / 2MS, containing 30mg L -1 Sucrose, 7mg·L -1 Agar, also contains 0.1mg·L -1 NAA or IBA, pH 5.6-5.7. The culture conditions of test tube seedlings are: temperature 25-28 ℃, light 16h d -1 , light intensity 1500-1800Lux. When the test tube seedling grows to 2-3cm, it can be directly cut off and transferred to the rooting medium for rooting cult...

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Abstract

The invention relates to a method for detoxifying and reproducing helianthus tuberosus quickly. The method comprises the following steps of: disinfecting young axillary buds or stem tips serving as explants, transferring the young axillary buds or stem tips into a bud induction culture medium, transferring the buds into a rooting culture medium, and performing refining seedlings and transplanting on a complete plant, wherein the bud induction culture medium is improved on the basis of a Murashige and Skoog (MS) culture medium, 0.1 mg.L<-1> of naphthyl acetic acid (NAA) and 0.5 mg.L<-1> of 6-butyl acrylate (BA) are added, so that adventitious buds which are induced are more, callus is small, and the problems of vitrification and water stain are solved; and obtained seedlings are robust and vigorous in growth vigor and can be transferred into the rooting culture medium directly, the rooting rate is 100 percent, and the survival rate of test-tube seedlings is over 90 percent. By the method, the effect of detoxifying the helianthus tuberosus seedlings can be achieved, the reproduction efficiency is improved, the production period is shortened, and an effective path is provided for the large-scale seedling raising of the helianthus tuberosus.

Description

technical field [0001] The invention belongs to the field of plant detoxification and rapid propagation, in particular to a method for detoxification and rapid propagation of Jerusalem artichoke. Background technique [0002] Jerusalem artichoke is a cultivated species that can form underground tubers in the genus Sunflower of the family Compositae. It is also known as sea ginger, sea ginger, devil ginger, etc., and its scientific name is Helianthus tuberosus L. It is widely used in tropical, temperate, cold, and arid and semi-arid regions of the world There are distribution and cultivation of Jerusalem artichoke. Jerusalem artichoke has strong adaptability, likes warmth, but is cold-resistant; likes humidity, but is drought-tolerant; likes fertility, but is resistant to barrenness, and salt-alkali. The fresh weight of Jerusalem artichoke biomass is generally 4-8 tons / mu, of which the output of the aboveground part is 2-3 tons / mu, and the underground tuber part is 2-5 tons / m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 隆小华严德凯刘玲刘兆普
Owner 北京碧青园生态环境科技有限公司
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