Method for quickly measuring soluble sugar in fruit

Abstract

A method for quickly measuring soluble sugar in fruit includes the following the steps of firstly, extracting soluble sugar of a sample, adding 500-1000 uL pure water to 50-150 mg pulp of fruit to be ground into homogenate, heating to kill activity of carbohydrate metabolic enzymes, and extracting supernate after centrifuging; secondly, pre-treating sample extract, and absorbing to remove impurities of anthocyanin, lutein and the like in the fruit by a solid phase extraction column; thirdly, preparing solution of high-purity standards, weighing monosaccharide, disaccharide, trisaccharide samples, and preparing a series of sugar standard solutions of different concentrations; fourthly, measuring purity of extract by ESI (electrospray ionization)-MS (mass spectrum), and removing impurities if needed; fifthly, detecting MALDI (matrix-assisted laser desorption ionization)-TOF (time of flight), performing mass spectrum scanning, and reading and recording peak area; sixthly, creating sugar standard curves for quantitative analysis of sugar; and seventhly, performing qualitative and quantitative analysis of sugar samples. The method is used for measuring composition and content of soluble sugar in fruit, steps are few, and the method is flexible and quick and can also be used for dynamic monitoring of sugar in development process of fruit.

Description

technical field [0001] The invention relates to a qualitative and quantitative determination method of carbohydrates. Background technique [0002] Fruit soluble solids refer to sugars, acids, vitamins, minerals, etc. that can be dissolved in water. The content of soluble solids can be roughly estimated by using a refractometer, but the sugar component, that is, the composition ratio of different sugars, cannot be detected by this method, and the sugar composition ratio is a common concern of growers and researchers, because it is directly related to the quality of fruit. Food quality and product quality. The soluble sugars in fruits are mainly hexose and disaccharides. The extraction method has been using the organic solvent method: use methanol / chloroform to extract the sugar in the fruit, then use the extraction method to remove the chloroform, use a rotary evaporator to volatilize the methanol, and use it after constant volume. Impurities such as pigments in the extrac...

AI Technical Summary

Problems solved by technology

HPLC has been widely used in medicine, chemistry and other fields, but it takes a long time to measure sugar by HPLC. Usually, the determination time of a single sample takes about 20 minutes, and generally only 3 samples can be measured in 1 hour; and it needs to use acetonitrile, Poisonous and harmful reagents such as methanol are used as mobile phases, which will pose potential health threats to operators

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